Development Years Experience

Ning Pan

** ***** ******, *****, ** *****

Home: 978-***-****

E-mail: ning,*******@*****.***

PROFILE

Analytical scientist with 14 years experience in pharmaceutical drug discovery and development.

Expertise in LC/MS data acquisition and analysis, HPLC and SFC achiral/chiral analysis as well as

purification.

EXPERIENCE

2000 – 2011

Pfizer/Wyeth, Cambridge, MA

I/I Chemistry/Discovery Analytical Sciences

Scientist (2009-2011)

Research Scientist II (2004-2009)

Research Scientist (2000-2004)

Provided analytical expertise to support Pfizer/Wyeth drug discovery function at Cambridge site in

following areas: 1) compound characterization by LC/MS, 2) purity determination by HPLC and SFC, 3)

milligram to gram scale purification by preparative HPLC, SFC, and automated preparative LC/MS

system, 4) supporting an open-access lab equipped with LC/MS, HPLC and preparative HPLC systems.

Performed LC/MS analysis daily with Waters LCT Premier and Agilent MSD to characterize final

compounds, synthetic intermediates as well as high throughput screening candidates. Contributed to

therapeutic research by developing and executing LC/MS methods to resolving project related

issues, such as determination of hydrolysis rate of pro-drug candidates in biological matrix.

Trained by Waters on Micromass LCT and Waters ZQ as well as Synapt G2 HDMS systems on

instrument operation, maintenance and problem troubleshooting.

Provided method development expertise in NP-HPLC, RP-HPLC, and SFC for achiral and chiral

analysis. Developed methods for purity and chiral purity determination of many challenging

compounds. Performed stability studies to support drug discovery projects. Summarized the results

and wrote the reports.

Have extensive method development experience in preparative purification. Purified compounds

with HPLC/MS for challenging compounds, such as compounds for toxicity studies, or degradation

products.

Trained and certified by Waters on operating Thar SFC/MS and Berger multi-gram preparative SFC

system. Developed and executed SFC methods for chiral purification of novel compounds to

support high priority projects.

After merger with Pfizer, implemented and operated Waters Auto Purification System to support

Immunology/Inflammation Chemistry on regular basis. This automated system allows direct scale

up from analytical testing to LC/MS based purification for batch process.

Supported an open-access analytical laboratory equipped with Waters LCT-Premier, Waters ZQ,

Agilent MSD, and Waters preparative HPLC systems. Developed and implemented new methods in

open access lab, performed routine instrument maintenance and problem solving, and provided

training to chemists.

Interacted with Medicinal Chemistry Department and Therapeutic Discovery Department very

closely for coordination of testing timelines and provided training to medicinal chemists on open

access systems.

Ning Pan 978-***-**** page 2 of 3

1998– 2000

Eli Lilly & Company, Indianapolis, IN

Biopharmaceutical Development

Analytical Chemist

Developed and validated separation-based analytical methods such as SEC, RP-HPLC, IE-HPLC,

and Peptide Mapping for release and stability testing of new biopharmaceutical compounds.

Performed product characterizations on reference standards, bulk drug substances, and formulated

drug products with SEC, RP-HPLC, IE-HPLC and other analytical technologies.

Established the stability testing procedures as well as method validation and transfer protocols,

summarized testing results and wrote technical reports on analytical method development, and

method validation.

Conducted accelerated stability studies and provided trend analysis for main peak and product-

related impurities.

Worked closely with formulation development groups and responsible for providing HPLC-based

analytical supports.

Familiar with ICH guidelines and cGMP regulations related to biopharmaceutical testing. Provided

scientific data for IND filings.

1996 – 1998

Fujisawa USA, Inc., Malrose Park, IL

Pharmaceutical Sciences

Associate Scientist

Developed and validated HPLC methods for drug substance and drug product release and stability

testing of small molecule candidates under cGMP environment.

Conducted stability studies for drug substance and drug product and provided trend analysis for active

ingredient and related impurities. Characterized reference standards and drug product with various

analytical technologies. Performed analytical method transfers between Analytical and QC labs.

Prepared technical protocols, reports, and analytical testing procedures. Prepared documents for FDA

submissions

Skilled in a variety of analytical techniques such as HPLC, IC, GC, FTIR, UV/VIS, and Karl Fisher.

Provided training for new lab scientists

Abbott Laboratories, North Chicago, IL 1994 - 1994

Corporate Industrial Hygiene

Lab Technician

Developed and validated sensitive HPLC methods for the determination of trace level

pharmaceutical compounds in aerosol in manufacturing facility.

Optimized separation conditions and improved detection sensitivity for HPLC methods.

1991 – 1993

University of Iowa, Iowa City, IA

Department of Chemistry

Teaching and Research Assistant

Conducted M.S. thesis research at Professor Donald J. Pietrzyk's research lab. Studied the retention

behavior of anionic surfactants on different polymer based anion exchangers.

Determined the parameters that affect the retention time, selectivity, and resolution of anionic

surfactants. Optimized the separation conditions for surfactant mixtures containing homolog and

positional isomers.

Ning Pan 978-***-**** page 3 of 3

Supervised undergraduate analytical laboratory

1984 – 1989

Northern Institute of Technology, Beijing, PRC

Department of Chemistry

Instructor

Instructed undergraduate courses of Analytical Chemistry and Instrumental Analysis

Supervised undergraduate analytical laboratory

EDUCATION

M.S. Analytical Chemistry University of Iowa

B.S. Chemistry Beijing Normal University

AWARDS

Chemical Screening Science Above and Beyond Award, Wyeth, 2004

Employment of the Month, Fujisawa USA

PUBLICATIONS

1. Xiang, Jason; Ipek, Manus; Suri, Vipin; Massefski, Walt; Pan, Ning; Ge, Ying; Tam, May; Xing,

Yuzhe; Tobin, James F.; Xu, Xin; Tam, Steve, “Synthesis and biological evaluation of

sulfonamidooxazoles and β-keto sulfones: selective inhibitors of 11β -hydroxysteroid dehydrogenase

type I”, Bioorganic & Medicinal Chemistry Letters 15(11), 2865-2869, 2005.

2. Ramarao, Manjunath K.; Murphy, Elizabeth A.; Shen, Marina W. H.; Wang, Yuren; Bushell, Kristen

N.; Huang, Nelson; Pan, Ning; Williams, Cara; Clark, James D., “A fluorescence-based assay for

fatty acid amide hydrolase compatible with high-throughput screening”, Analytical Biochemistry

343(1), 143-151, 2005.

3. Pan, N., Pietrzyk, D.J., “Retention of Anionic Surfactants on Anion Exchangers”, J. of

Chromatography. A, 706, 327-337, 1995.

PRESENTATIONS

1. Pan, Ning; Huang, Nelson; McConnell, Oliver; “LC/MS analysis of pharmaceuticals using

multimode ionization source”, Conference on Small Molecule Science, Chapel Hill, NC, July 2007.

2. Huang, Nelson; Bourassa, Jim; Ge, Ying; Pan,Ning; Xiang, Jason; Suri,Vipin; Zhang, Yan-Ling;

“LC/MS and LC/MS/MS Analyses for Screening and Characterization of 11b-Hydroxysteroid

Dehydrogenase Type I (11b-HSD1) Inhibitors”, ASMS, San Antonio, TX, June 2005.

3. Huang, Nelson; Massefski, Walt; Pan, Ning; McConnell, Oliver; “Evaluation of electrochemcial

oxidation as a tool for studying phase I metabolism and metabolic stability, Part II: Purification and

structural characterization of the oxidative products”, Conference on Small Molecule Science,

Bristol, RI, August 2005.

4. Huang, Nelson; Bourassa, Jim; Massefski, Walt; Pan, Ning; Wan, Anna; McConnell, Oliver;

“Evaluation of electrochemcial oxidation as a tool for phase I metabolism and metabolic s tability

studies”, Conference on Small Molecule Science, Bristol, RI, August 2004.

5. Pan, Ning; Lee, Jinbo; Kung, Leslie; Huang, Nelson; “HPLC stability analysis of drug candidates

from discovery research” 32nd Northeast Regional Meeting of the ACS, Rochester, NY,

October 2004.

Contact this candidate