Engineer Engineering

Rohan Degaonkar

*** ********** ***., *** *., Lowell, MA 01854

908-***-**** abgrtu@r.postjobfree.com

SUMMARY

Graduate student pursuing Master’s in Biological Science specializing in Biotechnology. Three years of

research experience in biotechnology industry as well as academia. Broad skill set and knowledge in

mammalian cell culture, molecular biology, and biochemistry.

EDUCATION

University of Massachusetts at Lowell, MA

Master’s Degree in Biological Sciences-Biotechnology (December 2012)

Coursework: Cell Culture, Molecular Biology, Biochemistry, Immunology, Advanced Protein Chemistry,

Isolation and purification of biotech products, and Process analytical technology.

K.I.T.’s College of Engineering, Kolhapur, India

Bachelor of Engineering in Biotechnology (June 2007)

Coursework: Microbiology, Fermentation Technology, Bioprocess Engineering, Genetic Engineering,

Unit Operations, Pharmaceutical Biotechnology.

PROFESSIONAL EXPERIENCE

Graduate Internship -Hepregen Corporation, Medford MA (May 2012-August 2012)

TM

Prepared Hepatopac plates using different donors of hepatocyte

Media preparation, freeze thawing, shake flask, and maintenance of primary hepatocyte culture.

Cultured the 96 well micropatterned plates with primary hepatocyte cells and fibroblasts, and

maintained the cells.

Dosed the cells with different inducers and repressors of cytochrome-p450 (CYP3A4) and

performed functional characterization like CYP3A4 promega glo P450 assay, albumin secretion

assay, and urea synthesis assay.

Transporter Characterization in HepatopacTM platform.

Uptake and efflux study of 4 different drug compounds on HepatopacTM cell culture platform.

Perform RT-PCR based gene expression of 6 different genes (NTCP, OCT1, MRP5, MDR1, BSEP, Actin)

coding for a particular transporter molecule. Performed functional characterization of HepatopacTM.

Compared the gene expression of HepatopacTM culture platform with the suspension culture.

Study the effect of kupffer cell mediated cell toxicity on HepatopacTM

Co-cultured kupffer cells on HepatopacTM, and studied the effects of two different compounds

diclofenac and troglitazone on HepatopacTM and kupffer cell cultured HepatopacTM.

Performed functional characterization on both the cell types and also performed GSH and ATP

assay to test cell toxicity and viability respectively.

Graduate Internship - Neo-Advent Technologies LLC Littleton, MA (Feb 2012- May 2012)

Performed drug formulation techniques for the key drug forms, characterized the active

pharmaceutical ingredients.

Performed particle size analysis, principles of the stability studies and stability experiments.

Proficient in liquid sample handling, including pipetting, transfer, serial dilution, and solution

preparation.

Graduate Student Assistant - University of Massachusetts at Lowell MA (Feb 2011 – Feb 2012)

Performed in-vitro cell based assays to measure toxicity endpoints of different nanomaterial

dispersions. Performed cytokine luminex assay, MTT cell proliferation assay and GSH/GSSG as part

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Rohan Degaonkar

117 University Ave., Apt 2., Lowell, MA 01854

908-***-**** abgrtu@r.postjobfree.com

of functional assays.

Performed maintenance, passage, isolation of mammalian cells (THP-1, NB4, Nb2a, and AML).

Successfully performed dispersion characterization of engineered nanomaterials in cell culture

medium using qNANO particle size analyzer and DLS.

Laboratory inventory management, documentation and notebook maintenance.

Technical support engineer - geneOmbio Technologies Pvt. Ltd., India (Jan 2008 – June 2009)

Performed basic molecular biology techniques like real-time PCR in quantitative measurement of

infectious diseases on various biological samples such as blood, plasma, CSF and tissue.

Assisted biotechnology distributers in developing strategic sales objectives; developed customized

preparations based on opportunity analysis; provided support at conferences, trade shows and

user group meetings.

SKILLS

Biochemistry: buffer preparation and titration of amino acid, enzyme assay and protein

determination by Bradford method, isolation and purification of enzyme

Cell based assays: ELISA, GSH/GSSG, cytokine multiplex, MTT cell proliferation assay, SDS PAGE, Bio-

Rad, albumin secretion, CYP3A4 induction assay, urea synthesis and ATP assay

Molecular Biology: Plasmid purification, RE digestion analyses of putative clones, colony PCR of

putative clones; agarose gel analysis of RE plasmids and PCR products, southern blot, DNA

concentration, DNA sequencing reactions, transformation for expression

Cell culture: cryopreservation, snap thawing, inducing differentiation, transient transfection of

mammalian cells, generation of adult stem cells from murine bone marrow, immunohistochemistry,

co-culture of hepatocyte and fibroblast, wright and giemsa staining, confocal microscopy

Immunology: ELISA immunoassay for determination of antigen titer, column chromatography-DEAE

agarose AFFI gel for separation of IgG from other immunoglobulins, dialysis and ammonium sulfate

precipitation of serum proteins, boost mice and perform cytotoxicity assay, hybridoma production

e.g., mouse spleen isolation and fusion with NS-1 myeloma cells

Characterization techniques: qNANO single particle analyzer, luminometer, DLS, real-time PCR,

luminex xMAP technology, sonicator, cyto-centrifugation, UV-vis plate reader, BET analysis, tapped

density tester, flowdex measurement, HPLC

Computer skills: Izon Control suite 2.1, SoftMax Pro 5.4, Luminex 100 TM IS v 1.7, SIMCA-P, Graphpad

PRISM, Mastercycler® ep realplex. Microsoft Office suite (Excel, Word, PowerPoint)

Data Analysis: perform multivariate analysis and design of experiment using SIMCA-P. PLS, PCA

model design using SIMCA-P tools

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