Namratha R Venkatesh- CV
Contact Information:
East Setauket, NY 11733
PH 678-***-****
Email: **********@*****.***
Education:
-Pursued a Bachelor of Engineering Degree (2006-10) from BMS College of Engineering,
Bangalore, INDIA, and graduated with Class Distinction in Medical Electronics.
-Currently pursued a Masters in Biomedical Engineering in Stony Brook University, 2012.
GPA: 3.4
AIM:
TITLE- Laboratory Researcher/Technician
To be a part of associated research community and serve allied concerns of my current research
knowledge in new targeted areas, to recognize my strengths and aptitudes and protract my work
into doctoral prospects. I have the visibility in learning new technologies with an open mind and
can adapt to handling bigger newer projects and hence expanding my knowledge base.
Research Experience:
Undergraduate Research
I have worked on an Undergraduate project pertaining to Cellular Organization and Signaling at
the NCBS (National Centre for Biological Sciences), Bangalore- here I worked in a lab that
focused on exploring the different outlets of endocytosis in terms of intake, new techniques in
fluorescence microscopy were used to visualize in vivo nano-structure and the activity of these
components. The physico chemical nature of the plasma membrane was the center point where
the aim was to look at the patches that concentrate lipids and proteins and to find out if they are
smaller than observed before. Next, we had been fleshing out the molecular basis of a novel
mode of endocytosis in which these patches are used but in which, membrane is pinched off as
vesicles without recourse to the girdling protein dynamin. The crucial significance of endocytic
intake, cellular eavesdropping, for the whole organism is also being examined, in the model
genetic system, Drosophila, and in the human immune system.
Graduate research- Biomaterial (Nanoscience) studies
My Graduate research at Sitharaman lab (BME) Stony Brook has involved material
synthesis techniques, physico-chemical characterization techniques, tissue culture and in
vivo studies in nanosystems laboratory.
Much emphasis has been laid on graphene, nothing but a single layer of graphite which
has high crystallinity and electronic properties which show promise in being used in the
fabrication of nanoscale devices. Graphene has been synthesized as thin ribbons that are
transformed from semiconductors to semimetals. The ribbons upon synthesis faced issues
of entanglement which prevented it from completely encapsulated. These problems needed
changes in the protocol which perhaps owed it to factors concerning oxidizing and reducing
agents involved. Rate of degradation of nanoribbons in vivo were tested using cheaper and
more efficient enzymes such as Lignin peroxidase and mechanism of its degradation were
analyzed using AFM and TEM.
In microwave heating experiments, it was observed that significantly greater temperature
increases in mixtures containing SWCNTs. These temperature increases scaled linearly
with the effective conductivity of the mixtures. The results of these experiments suggest that
SWCNTs may enhance contrast for microwave imaging and facilitate selective microwave
heating for treatment of breast cancer. Results of study on MWCNTs lead to us being a
little skeptical about the possibility of achieving better answers with SWCNTs. The
potential for SWCNTs to have dielectric properties that will facilitate microwave detection
can be used magnanimously in medical applications including cancer treatment.
Human and murine cell studies were used to exploit cytotoxicity assays and they were
conducted wherein functionalized nanoribbons were used to target these cells. The assays
used included LDH, Trypan Blue, and Alamar blue to determine live-dead condition of
cells.
Lab Techniques proficient in: Synthesis and Characterization of nanoribbons using
microscopy techniques such as Atomic Force Microscopy, Transmission Electron
Microscopy, and Scanning Electron Microscopy, Dynamic Light Scattering (DLS), Raman
spectroscopy and UV-vis spectroscopy,HPLC and basic Fluorescence Spectroscopy
methods. Advanced Cell Culture studies using human, murine and bacterial cells- Protein
transfection studies, PCR, DNA sequencing, Western Blotting, PLGA and so on.
Previous Experience
--Worked as Research Aide in Department of Pediatrics, Stony Brook University Hospital,
summer 2012.
-- Worked as a Research Assistant in BME department, Sitharaman Lab for fall 2011 and spring
2012.
Computer Languages: C, C++, VHDL, Verilog, Python, Matlab and softwares such as SQL,
BIOPAC. Proficient in Microsoft Office.