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Plant C

Location:
Pune, MH, India
Posted:
December 27, 2012

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Resume:

CURRICULUM VITAE

DR. ZAMBARE VASUDEO P.

(M.Sc., Ph.D., FISBT, FSLSc, FSASc, FIIOAB, FSAB, MSFSN)

E-Mail: ***********@*****.***

Cell: +91-922*******; Landline: +91-253-*******

General information

Address for Correspondence 18, Abhish Nagar Bldg. No. 4, Shivaji Nagar, Nasik-Pune Road, Nasik

422006, Maharashtra, India.

Sex Male

Marital status Married

Qualification M.Sc., Ph.D., FISBT, FSLSc, FSASc, FIIOAB, FSAB, MSFSN

Educational Background

1. Ph. D. -Biochemistry (2007) Agharkar Research Institute, University of Pune, Pune (India)

Topic: Application of Microbial Proteases in Leather processing

Research Guide: Dr. Mrs. S. S. Nilegaonkar

2. M.Sc.-Biochemistry (2001) North Maharashtra University, Jalgaon (India).

3. B.Sc.-Chemistry (1999) North Maharashtra University, Jalgaon (India).

Employment History

June 2012 to Present Vice President (Biotechnology Division), Powerdeal Energy Systems (I) Pvt.

Ltd, Survey No. 4/1, 5/1 & 6/1, Villoli, Mumbai Agra Road, Nasik 422010,

Maharashtra, India

Nov. 2011 to May 2012 General Manager (Operations and R&D) @ Sequence Biotech Pvt. Ltd,

Nasik 422403, India

June, 2009 to Present Technical Director (R&D) @ Jai Biotech Industries, Nasik 422007, India

Jul. 2009 to October 2011 Research Scientist-I @ South Dakota School of Mines and Technology

(SDSMT), Centre for Bioprocessing Research and Development (CBRD), Rapid

City, South Dakota 57701, USA.

Nov. 2007 to Jun. 2009 Research Scientist @ R & D Department, Advanced Enzyme Technologies

Ltd. A61/62, M.I.D.C., Malegaon, Tal. Sinner, Dist. Nashik, 422113,

Maharashtra State, INDIA.

Jul. 2007 to Nov. 2007 Lecturer @ P. G. Department of Biochemistry, New Arts, Commerce &

Science College, Ahmednagar 414001 INDIA.

Jan. 2002 to May 2002 Microbiologist @ Department of Microbiology, Central Laboratory, Jain Food

Park, Jalgaon, 425001 INDIA.

Research Experience

Post Doctoral

Working on “Discovery of extremozymes from extremophiles isolated from natural resources for

1.

industrial application”. (Jul 2009 to October 2011).

Extremophilic microbes produced extremozymes which have potential use for several industrial applications. Various

extremophiles were isolated from lake, yard waste compost, hot springs, Yellowstone National park, DUSEL.

Extremophiles have potential in cellulase and xylanases production using lignocellulosic substrates for fermentable sugar

production followed by ethanol fermentation. Simultaneous saccharification & fermentation (SSF) and separate hydrolysis

& fermentation (SHF) for biofuel production were carried out with experimental and commercial enzymes. I worked on

biofuel and bioenergy production including- bioethanol, biohydrogen, biodiesel and bioelectricity through microbial fuel

cell. Initiated research work on lichen as a source of microbes for bioactive component and biodiesel production .

Worked on “Strain improvement for extracellular enzyme of various bacteria and fungi using

2.

classical mutation” (Nov. 2007-Jul 2009).

Several extracellular enzymes including amylase, amyloglucosidase, protease, serratiopeptidase, cellulase, xylanases,

lactase and phytase were produced from various bacteria and fungi. Classical mutation by physical and chemical agent

employed for strain improvement of all enzymes from various microbes. Strains were isolated and were studied for several

generations with various cultural and nutritional parameters. Potential enzymes were characterized by HPLC, 2D gel

electrophoresis.

Academic

Worked on CSIR- NMITLI project “Biotechnology for leather: Towards cleaner processing Phase I”

3.

since 24 July 2002 to 28 February 2005.

th th

Worked on CSIR- NMITLI project “Biotechnology for leather: Towards cleaner processing Phase II”

4.

since 1 March 2005 to 27 July 2007.

st th

Soil-1 and BSA 26, dehairing protease-producing microorganisms were isolated from vermicompost pit soil and buffalo

hide respectively. Both the cultures were identified according to the Bergey’s Manual of Systematic Bac teriology and by

molecular technique of 16 S rDNA. Optimization of protease production was carried out with different cultural conditions

(aerobic- anaerobic, shake- steady, shaking speed, cultivation volume); environmental parameters (pH, temperature and %

inoculum size); nutritional parameters (carbon, nitrogen sources, inorganic salts, agro products/ byproducts, non ionic

surfactants- detergents, antifoaming agents). A statistical tool of response surface methodology (RSM) was also studied for

optimization of final ingredient concentrations. Production optimization of protease was carried out at 10L fermentor with

respect to agitation and aeration. Scale up of protease production was carried out at 100L fermentor. The protease was

purified by ammonium sulphate precipitation, gel filtration and preparative gel electrophoresis and characterized. The

crude enzyme has potential application in soaking, dehairing and bating operations of leather processing. In addition it has

application in detergent industry. It has extensive application in recovery of silver from waste X- ray and photographic

films.

Worked on ARI Project MIC-18 “Improvement of protease production by alkalophilic bacteria” since

5.

11 May 2002 to 23 July 2002.

th rd

Lonar lake isolate, Bacillus sphaericus, was tested for protease production followed by media optimization with respect to

various cultural, environmental and nutritional parameters. The protease was purified by ammonium sulphate precipitation,

gel filtration and DEAE sepharose and characterized. The gene responsible for protease production was cloned in E. coli

strain and studied for its expression. The crude enzyme has potential application in detergent industry.

Worked on “Adjuvant therapy of Plumbago zeylanica against UTI microorganisms” (2000- 2001)

6.

M.Sc. part II Dissertation.

Antibiotic resistant pattern is developed in many microbes, especially in enterobacters. Antibiotic resistance pattern was

done. To solve the problems use of plant based natural products will be the best so lution. Plumbagin, a napthquinonic

compound extracted and characterized from plant Plumbago zeylanica. Various extracts were evaluated for antimicrobial

activity against pathogenic bacteria like E. coli, Salmonella sp., Shigella sp., Klaibsiella pneumonae, S. aureus and P.

aeruginosa. Plant extract was used as a curing agent in treatment of infectious diseases at plasmid DNA level.

Industrial

7. Worked on bacterial/fungal fermentation (solid state and submerged state), media designing, process optimization, and

strain improvement using rational, statistical and classical mutational approaches for microbial enzyme production,

fermentation scaled up from flask level to 1000L fermentor . Also as a advisor with one of Indian Agricultural Company,

we developed total 6 products (1-fungicide from Trichoderma viridae, 4-bioinsecticide from Beauveria bassiana,

Verticillium becanii, Metarhizium anisopliae, Pseudomonas fluorescence and 1- nematocide from Paecilomyces

lilancinus).

2

Teaching Experience

1. Worked as Lecturer in P. G. Department of Biochemistry, new Arts, Commerce & Science College,

Ahmednagar 414001, Maharashtra state, India (Jul. 28, 2007 to Nov.10, 2007).

2. Worked as Visiting Faculty at P. G. Department of Biochemistry, K.T.H.M. College, Nasik, Maharashtra

state, India (August 2008-Dec. 2008)

3. Invited as Guest Lecturer at Department of Microbiology, M. J. College, Jalgaon, Maharashtra state,

India.

4. Invited as Guest Lecturer at Department of Microbiology, Modern College, Ganeshkhind, Pune,

Maharashtra state, India.

5. Invited as Guest Lecturer at Department of Microbiology, Abeda Inamdar College for Girls College,

Pune, Maharashtra state, India.

6. Invited as Guest Lecturer at P. G. Department of Biochemistry, K.T.H.M. College, Nasik, Maharashtra

state, India.

Publications…Research Papers in Journals

Published (Total 43)

2007

1. S. S. Nilegaonkar, V. P. Zambare, P. P. Kanekar, P. K. Dhakephalkar and S. S. Sarnaik. 2007. Production

of dehairing protease by Bacillus cereus MCM B- 326. Bioresource Technology 98 (6): 1238-1245

(Impact Factor- 4.253).

2. V. P. Zambare, S. S. Nilegaonkar and P. P. Kanekar. 2007. Production of an alkaline protease and its

application in dehairing of baffalo hide. World Journal of Microbiology and Biotechnology 23 (11): 1569-

1574 (Impact Factor- 1.082).

3. V. P. Zambare*, P. S. Kothari, M. V. Kulkarni. 2007. Plasmid curing of plumbagin and its application in

bacterial antibiotic resistance. Journal of Pure and Applied Microbiology 1(2): 285-288.

4. V. P. Zambare*and A. A. Bhole. 2007. In vitro antifungal activity of Plumbago zeylanica. Journal of Pure

and Applied Microbiology 1 (2): 325-326.

2008

5. M. V. Padul and V. P. Zambare*. In vitro antibacterial activity of Enicostema littorale plant extracts.

Journal of Pure and Applied Microbiology 2 (1): 245-247.

6. V. P. Zambare* and A. A. Bhole. 2008. In vitro antioxidant activity of Plumbago zeylanica. Research

Journal of BioTechnology. 3(3): 36-37 (Impact Factor- 0.174).

7. V. P. Zambare*, M. V. Padul, Yadav A. A. and Shete T. B. 2008. Vermiwash: b iochemical and

microbiological approach as eco-friendly soil conditioner. Journal of Agricultural and Biological Sciences

3(4): 1-5.

8. Zambare V.P.*and Bhoyte S. A. 2008. Antibacterial activity of ginger ( Zingiber officinalis). Journal of

Pure and Applied Microbiology. 2(2): 591-593.

2009

3

9. Rasika Pawar, Vasudeo Zambare*, Siddhivinayak Barve and Govind Paratkar. 2009 Application of

protease isolated from Bacillus sp.-158 in enzymatic cleaning of contact lenses. Biotechnology 8 (2): 276-

280.

10. V. P. Zambare* 2009. Antibiotic resistance and sensitivity pattern of pathogenic bacteria isolated from

urinary tract infected sample. Biomedical and Pharmacology Journal 2(1): 137-140.

11. Zambare V. P.*and Bhoyte S. A. 2009. Antibacterial activity of tea. Biomedical and Pharmacology

Journal 2(1): 173-175.

2010

12. V. P. Zambare* 2010. Solid state fermentation production of cellulase from Bacillus sp. International

Journal of BioScience, Agriculture and Technology, 2(1): 1-6.

13. Vasudeo Zambare* 2010. Solid state fermentation of Aspergillus oryzae for glucoamylase production on

agro residues. Accepted to International Journal of Life Sciences.4: 16-25.

14. Vasudeo Zambare*2010. Strain improvement of alkaline protease from Trichoderma reesei MTCC-3929

by physical and chemical mutagen. The IIOAB Journal, 1(1): 25-28.

15. V. P. Zambare* 2010. Optimization of nutritional factors for extracellular protease production from

Bacillus sp. using response surface resistance. International Journal of BioEngineering and Technology

1(1): 43-47.

16. V.P. Zambare*, Lew P. Christopher 2010. Solid state fermentation and characterization of a cellulase

enzyme system from Aspergillus niger SB-2. International Journal of Biological Sciences and Technology

2(3): 22-29.

17. Vasudeo Zambare*, Archana Zambare, Lew Christopher 2010. Antioxidant and antibacterial activity of

extracts from lichen Xanthoparmelia somloensis, native to the Black Hills, South Dakota, USA.

International Journal Medical Science and Technology 3(7): 46-51.

18. Vasudeo Zambare, Smita Nilegaonkar and Pradnya Kanekar 2010. Application of protease from Bacillus

cereus MCM B-326 as a bating agent in leather processing. The IIOAB Journal 1(3): 18-21.

19. Vasudeo Zambare* 2010. Purification and characterization of neutral serine protease from Bacillus sp.

Asiatic Journal of Biotechnology Resources 3: 183-192.

2011

20. V.P. Zambare, A. Bhalla, K. Muthukumarappan, R. Sani, L. Christopher. 2011. Bioprocessing of

agricultural residues to ethanol utilizing a cellulolytic extremophile. Extremophiles 15: 611-618 (Impact

Factor- 2.16).

21. V. P. Zambare, S. S. Nilegaonkar and P. P. Kanekar. 2011. Production optimization and purification of a

novel extracellular protease from Pseudomonas aeruginosa MCM B-327. New Biotechnology 28 (2): 173-

181 (Impact Factor- 2.0).

22. Zambare V, Zambare A, Muthukumarappan K, Christopher LP. 2011. Potential of thermostable cellulases

in the bioprocessing of switchgrass to ethanol. BioResources 6(2): 2004-2020 (Impact Factor- 1.418).

23. V. P. Zambare, S. S. Nilegaonkar and P. P. Kanekar. 2011. Use of agroresidues for protease production

and application in degelatinazation. Research Journal of BioTechnology 6(2): 62-65 (Impact Factor-

0.284).

4

24. Vasudeo Zambare*, Archana Zambare, Kasiviswanathan Muthukumarappan, Lew Christopher 2011.

Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for

biomass bioprocessing. International Journal of Energy and Environment 2(1): 99-112.

25. Vasudeo Zambare* 2011. Optimization of amylase production from Bacillus sp. using statistics based

experimental design. Emirates Journal of Food and Agriculture 23 (1): 37-47.

26. V.P. Zambare*, Lew P. Christopher 2011. Statistical analysis of cellulase production in Bacillus

amyloliquefaciens UNPDV-22. Extreme Life, Biospeology & Astrobiology, 3(1): 38-45.

27. V.P. Zambare*, Lew P. Christopher. 2011. Optimization of culture conditions for cellulase production

from thermophilic Bacillus strain. Journal of Chemistry and Chemical Engineering 5(7): 521-527.

28. V.P. Zambare*, M. Ponraj, V. Rubeni, 2011. Prevalence and identification of toxic shock syndrome toxin

(TSST) producing wound isolates of Staphylococcus aureus from Namakkal District of Tamil Nadu.

BioChemistry: An Indian Journal 5(2): 1-9.

29. M. Ponraj, Gokila, V. Zambare* 2011. Bacterial decolorization of textile dye (Orange 3R). International

Journal of Advanced Biotechnology and Research 2(1): 168-177.

30. M. Ponraj, P. Jamunarani, V. Zambare* 2011. Isolation and optimization of conditions for decolorization

of true blue by textile dye decolorizing fungi. Asian Journal of Experimental and Biological Sciences

2(2):270-277.

31. Louise Teixeira Cerdeira, Anne Cybelle Pinto, Maria Paula Cruz Schneider, Sintia Silva de Almeida,

Anderson Rodrigues dos Santos, Eudes Guilherme Vieira Barbosa, Amjad Ali, Maria Silvanira Barbosa,

Adriana Ribeiro Carneiro, Rommel Thiago Jucá Ramos, Rodrigo Oliveira dos Santos, Debmalya Barh,

Neha Barve, Vasudeo Zambare, Silvia Estevão Belchior, Luis Carlos Guimarães, Siomar de Castro

Soares, Vinicius Augusto Carvalho de Abreu, Andreas Tauch, Eva Trost, Anderson Miyoshi, Vasco

Azevedo, Artur Silva (2011) Whole genome sequence of Corynebacterium pseudotuberculosis PAT10 of

strain isolated from sheep in Patagonia, Argentine. Journal of Bacteriology 193 (22): 6420-6421. (Impact

Factor- 3.726).

32. Louise Teixeira Cerdeira, Maria Paula Cruz Schneider, Anne Cybelle Pinto, Sintia Silva de Almeida,

Anderson Rodrigues dos Santos, Eudes Guilherme Vieira Barbosa, Amjad Ali, Fla via Figueira Aburjaile,

Vinicius Augusto Carvalho de Abreu, Luis Carlos Guimara es, Siomar de Castro Soares, Fernanda Alves

Dorella, Fla via Souza Rocha, Erick Bol, Pablo Henrique Caracciolo Gomes de Sa, Thiago Souza Lopes,

Maria Silvanira Barbosa, Adriana Ribeiro Carneiro, Rommel Thiago Juca Ramos, Nilson Antoˆnio da

Rocha Coimbra, Alex Ranieri Jeroˆnimo Lima, Debmalya Barh, N eha Jain, Sandeep Tiwari, Rathinam

Raja, Vasudeo Zambare, Preetam Ghosh, Eva Trost, Andreas Tauch, Anderson Miyoshi, Vasco Azevedo,

and Artur Silva (2011) Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain CIP

52.97, Isolated from a Horse in Kenya. Journal of Bacteriology 193 (24): 7025-7026 (Impact Factor-

3.726).

33. V.P. Zambare*, Lew P. Christopher (2011) Statistical optimization of process parameters for cellulase

production by thermophilic Bacillus amyloliquefaciens. International Journal of Food and Fermentation

Technology. 1(1): 63-68.

34. Gadge PP, Madhikar SD, Yewle JN, Jadhav UU, Chougale AD, Zambare VP, Padul MV. (2011)Isolation,

partial purification and characterization of lipase from soybean germinating oil seeds. American Journal of

Biochemistry and Biotechnology 7(3): 141-145 (Impact Factor 0.84).

5

35. Madhikar SD, Gadge PP, Yewle JN, Jadhav UU, Chougale AD, Zambare VP, Padul MV. Isolation,

partial purification and characterization of lipase from sunflower germinating oil seeds. International

Journal of Biotechnology and Bioscience 1(4): 410-415.

2012

36. V.P. Zambare, Lew P. Christopher (2012). Optimization of enzymatic hydrolysis of corn stover for

improved ethanol production. Energy Exploitation & Exploration 30(2): 193-205 (Impact Factor- 1.712).

37. Vasudeo Zambare, Archana Zambare, Debmalya Barh, Lew Christopher (2012) Optimization of

enzymatic hydrolysis of prairie cordgrass for improved ethanol production. Journal of Renewable and

Sustainable Energy 4 (3): 1-8 (Impact factor 1.214).

38. Axel Hollmann, Mariano Saviello, Lucrecia Delfederico, Tessália Diniz Luerce Saraiva, Debmalya Barh,

Neha Jain, Sandeep Tiwari, Sudha Chandra, Krishnakant Gupta, Vasudeo Zambare, Anil Kumar, Lew

Christopher, Vasco Azevedo, Liliana Semorile, Anderson Miyoshi (2012) Tight controlled expression and

secretion of Lactobacillus brevis SlpA in Lactococcus lactis. Biotechnology Letters 34(7): 1275-1281

(Impact factor 1.683).

39. V. P. Zambare, S. S. Nilegaonkar and P. P. Kanekar (2012) Optimization of Nutritional Factors for

Extracellular Amylase Production from Bacillus cereus MCM B-326 Using Response Surface

Methodology. Research Journal of BioTechnology 7(4): 58-65 (Impact Factor 0.143).

40. Din M.F.M., Ponraj M., Zaini U., Van Loosdrecht, M.C.M., Salmiati Y., Chelliapan S., Zambare V., G.

Olsen (2012) Development of Bio-PORecŽ System for polyhydroxyalkanoates (PHA) production and its

storage in mixed cultures of palm oil mill effluent (POME). Bioresource Technology 124: 208-216

(Impact Factor 4.980).

41. Syed Hassan, Maria Paula Schneider, Rommel Thiago Ra mos, Adriana Carneiro, Alex Ranieri Lima, Luis

Carlos Guimarães, Amjad Ali, Syeda Bakhtiar, Ulisses Pereira, Anderson Santos, Siomar de Castro Soares,

Fernanda Dorella, Anne Pinto, Dayana Ribeiro, Maria Silvanira Barbosa, Sintia Almeida, Vinicius Augusto

Abreu, Flavia Aburjaile, Karina Kelly Fiaux, Eudes Guilherme Barbosa, Carlos Diniz, Flavia Rocha,

Rashmi Saxena, Sandeep Tiwari, Vasudeo Zambare, Preetam Ghosh, Luis Gustavo Pacheco, Christopher

Dowson, Anil Kumar, Debmalya Barh, Anderson Miyoshi, Vasco Aze vedo, and Artur Silva (2012) Whole-

genome sequence of Corynebacterium pseudotuberculosis strain 162, isolated from Camel. Journal of

Bacteriology 194(20): 5718-5719 (Impact Factor 3.825).

42. V. P. Zambare, S. S. Nilegaonkar and P. P. Kanekar. 2012. Protease production and enzymatic soaking of

salt-preserved buffalo hides for leather processing. IIAOB Letters (Accepted).

43. V. P. Zambare, S. S. Nilegaonkar and P. P. Kanekar. 2012. Scale up production of protease using

Pseudomonas aeruginosa MCM B-327 and its detergent compatibility. SpringerPlus (Accepted).

Communicated (Total 4)

1. V.P. Zambare, Lew P. Christopher. Statistical optimization of culture condition for enhanced cellulase

production by Penicillium pinophilum ATCC 200401.

2. V.P. Zambare, Lew P. Christopher. Statistical optimization of culture condition for enhanced xylanase

production by Penicillium pinophilum ATCC 200401.

3. Archana Zambare, Vasudeo Zambare, Lew Christopher. Fermentation production optimization of

thermophilic lipase from Geobacillus thermodenitrificans AV-5 of Yellowstone National Park, Wyoming.

6

4. Archana Zambare, Vasudeo Zambare, Lew Christopher. Characterization of thermophilic lipase from

Geobacillus thermodenitrificans AV-5 of Yellowstone National Park, Wyoming.

* Corresponding authors.

Publications…Review Articles in Journals (2)

1. V.P. Zambare, Lew P. Christopher (2012). Biopharmaceutical potential of lichens. Pharmaceutical

Biology 50(6): 778-798 (Impact Factor 0.878).

2. Hemaiswarya S., Raja R., Carvalho Isabel S., Ravikumar R., Vasudeo Zambare and Debmalya Barh.

2012. An Indian scenario on renewable and sustainable energy sources with emphasis on algae. Applied

Microbiology and Biotechnology 96: 1125-1135 (Impact Factor 3.425).

Publications Books (Published-1, Submitted-1)

1. V. P. Zambare (2011) Plant Beverages: antioxidant and antimicrobial approach. Lambert Academic

Publishing, Germany (ISBN- 978-3-8454-2813-0).

2. D. Barh, V. Zambare, V. Azevedo. OMICS: Applications in Biomedical, Agriculture and Environmental

Sciences. CRC Press, Taylor and Francis, Germany (ISBN-10: 146*******, ISBN-13: 978-1466562813)

Publications Book Chapter (Total-3)

1. V. P. Zambare*, P. S. Kothari, M. V. Kulkarni (2004) Antibacterial activity in vitro of plumbagin isolated

from Plumbago zeylanica. In Biotechnological Approaches for Sustainable Development, M.S. Reddy and

S. Khanna (eds.) Allied Publishing Pvt. Ltd. New Delhi. (ISBN - 81-7764-669-9), pp-196-200.

2. S. S. Nilegaonkar, V. P. Zambare and P. P. Kanekar (2004) Extracellular protease from Bacillus sp. BSA-

26: application in dehairing of buffalo hide. In Biotechnological Approaches for Sustainable Development,

M.S. Reddy and S. Khanna (eds.) Allied Publishing Pvt. Ltd. New Delhi. (ISBN - 81-7764-669-9), pp-186-

191.

3. Vasudeo Zambare*, Archana Zambare, Lew Christopher (2011) Enzymatic hydrolysis of hemicellulose

from corn stover and prairie cordgrass. IN: Advances in Biotech Research, A.M. Deshmukh, J.D. Jawalikar

and S.S. Waghmare (eds), Microbiologist Society (India, Osmanabad and Education Book Pub lisher &

Distributor, Aurangabad, Maharashtra, India.6-9 February 2011, at Aurangabad, Maharashtra, India.

(ISBN: 978-93-80876-04-7), pp-300-313.

Publications Proceedings (Total-1)

1. Vasudeo Zambare* 2008. Biotechnological applications of proteases in leather processing: a green

technology. Proceedings of National Conference on Green Technology (NCGT-2008), 10-12 November

2008, In association with AICTE. Organized By Government Polytechnic, Nashik, India.

Publications Database (Total-12)

1. P. P. Kanekar, S. S. Nilegaonkar, http://www.ncbi.nlm.nih.gov.sites.entrez Nucleotide accession No. DQ

479314. V.P. Zambare, P.K. Dhakephalkar and S.S. Sarnaik (2006) Nucleotide sequence of Bacillus

cereus MCM B-326: 16S rRNA partial sequence. NCBI Database.

7

2. S. S. Nilegaonkar, http://www.ncbi.nlm.nih.gov.sites.entrez Nucleotide accession No. DQ 473435. V.P.

Zambare, P.P. Kanekar and P.K. Dhakephalkar (2006) Nucleotide sequence of Pseudomonas aeruginosa

MCM B-327: 16S rRNA partial sequence. NCBI Database.

3. V.P. Zambare http://www.ncbi.nlm.nih.gov.sites.entrez Nucleotide accession No. HQ 005305 (2010)

Nucleotide sequence of Bacillus amylolequifaciens UNPDV-22: 16S rRNA partial sequence. NCBI

Database.

4. Archana Zambare, Vasudeo Zambare, Lew Christopher (2011) Nucleotide Accession Number JN837496

for 16S rRNA partial nucleotide sequence of Geobacillus thermodenitrificans AV-5. NCBI Database

(http://www.ncbi.nlm.nih.gov.sites.entrez)

5. Cerdeira L, Schneider MPC, Barbosa MS, Ramos RTJ, Carneiro AR, Santos RS, Lima M, DAFonseca V,

Almeida SS, Santos AR, Soares SC, Pinto AC, Ali A, Barbosa E, Dorella FA, Rocha FS, Guimaraes LG,

Belchior SB, Ghosh P, Zambare V, Barve N, Tiwari S, Barh D, Miyoshi A, Azevedo V, Silva A (2011)

Corynebacterium pseudotuberculosis PAT10, complete genome sequence No. CP 00292 4. EMBL-EBI

Database http://www.ebi.ac.uk/ena/data/view/CP002924.

6. Cerdeira LT, Schneider MPC, Pinto AC, de Almeida SS, dos Santos AR, Barbosa EGV, Ali A, Aburjaile

FF, de Abreu VAC, Guimara es LC, de Castro Soares S, Dorella FA, Rocha FS, Bol E, de Sa PHCG,

Lopes TS, Barbosa MS, Carneiro AR, Ramos RTJ, Coimbra NAR, Lima ARJ, Barh D, Jain N, Tiwari S,

Raja R, Zambare V, Ghosh P, Trost E, Tauch A, Miyoshi A, Azevedo V and Silva A (2011)

Corynebacterium pseudotuberculosis Strain CIP 52.97, complete genome sequence from Horse in Kenya.

CP003061. EMBL-EBI Database: http://www.ncbi.nlm.nih.gov/nuccore/349734091

7. Silva,A., Ramos,R., Carneiro,A., Almeida,S., Barbosa,S.,Pinto,A.C., Cerdeira,L., Santos,A., Soares,S.,

Guimaraes,L., Barbosa,E., Figueira,F., Souza,F., Abreu,V.C., Dorella,F., Pacheco,L., Ghosh,P.,

Zambare,V., Barve,N., Tiwari,S., Barh,D., Miyoshi,A., Schneider,M.P. and Azevedo,V. (2012 )

Corynebacterium pseudotuberculosis 316, complete genome sequence No. CP003077. EMBL-EBI

Database: http://www.ncbi.nlm.nih.gov/nuccore/377652989

8. Silva,A., Cerdeira,L., Bol,E., Barbosa,M.S., Muller,B., Helden,P.V., Santos,A.R., Ramos,R.T.J.,

Carneiro,A.R., Guimaraes,L.C., Aburjaile,F.F., Padua,U.D., Barbosa,E., Fiaux,K.K., Diniz,C.A.A.,

Soares,S.C., Pinto,A.C., Almeida,S.S., Abreu,V.A.C., Hassan,S.S., Khatri,G., Rahangdale,S., Gupta,K.,

Verma,A.S., Ghosh,P., Zambare,V., Kumavath,R.N., Barh,D., Miyoshi,A., Schneider,M.P.C. and

Azevedo,V. (2012) Corynebacterium pseudotuberculosis P54B96, complete genome CP003385 EMBL-

EBI Database: http://www.ncbi.nlm.nih.gov/nuccore/380868692

9. Silva,A., Lopes,T., Ramos,R.T.J., Carneiro,A.R., Barbosa,M.S., Santos,A.R., Guimaraes,L.C.,

Aburjaile,F.F., Padua,Ud., Barbosa,E.,Fiaux,K.K., Diniz,C.A.A., Soares,S.C., Pinto,A.C., Almeida,S.S.,

Abreu,V.A.C., Hassan,S.S., Khatri,G., Rahangdale,S., Gupta, K., Verma,A., Kumavath, R.N., Ghosh,P.,

Zambare,V., Barh,D., Miyoshi,A., Schneider,M.P.C. and Azevedo,V. (2012) Corynebacterium

pseudotuberculosis 267, complete genome CP003407 EMBL-EBI Database:

http://www.ncbi.nlm.nih.gov/nuccore/CP003407.1

10. Hassan,S.S., Schneider,M.P., Ramos,R.T., Carneiro,A.R., Ranieri,A.,Guimaraes,L.C., Ali,A.,

Bakhtiar,S.M., Pereira Ude,P., Dos Santos,A.R., Soares Sde,C., Dorella,F., Pinto,A.C., Ribeiro,D.,

Barbosa,M.S., Almeida,S., Abreu,V., Aburjaile,F., Fiaux,K., Barbosa,E., Diniz,C., Rocha,F.S., Saxena,R.,

Tiwari,S., Zambare,V., Ghosh,P., Pacheco,L.G., Dowson,C.G., Kumar,A., Barh,D., Miyoshi, A.,

Azevedo,V. and Silva,A. Corynebacterium pseudotuberculosis Cp162, complete genome, complete

genome CP003652, EMBL-EBI Database http://www.ncbi.nlm.nih.gov/nuccore/CP003652.1

11. Silva,A., Saleh,M., Selim,S.A., Cerdeira,L., Ramos, R.T., Carneiro,A.R., Barbosa,S., Santos,A., Soares,S.,

Guimaraes,L., Diniz,C.A., Fiaux,K., Hassan,S.S., Ali,A., Barbosa,E., Figueira,F., Pinto,A.C., Almeida,S.,

Abreu,V.C., Dorella,F., Pacheco,L., Souza,F., Ghosh,P., Zambare,V., Barh,D., Miyoshi,A.,

Schneider,M.P. and Azevedo,V. (2012) Corynebacterium pseudotuberculosis 31 chromosome, complete

8

genome, complete genome, complete genome CP003421, EMBL-EBI Database

http://www.ncbi.nlm.nih.gov/nuccore/CP003421.1

12. Silva,A., Saleh,M., Selim,S.A., Cerdeira,L., Ramos,R.T., Carneiro,A.R., Barbosa,S., Santos,A., Soares,S.,

Guimaraes,L., Diniz,C.A., Fiaux,K., Hassan,S.S., Ali,A., Barbosa,E., Figueira,F., Pinto,A.C., Almeida,S.,

Abreu,V.C., Dorella,F., Pacheco,L., Souza,F., Ghosh,P., Zambare,V., Barh,D., Miyoshi,A.,

Schneider,M.P. and Azevedo,V. (2012) Corynebacterium pseudotuberculosis 258 chromosome, complete

genome, complete genome, complete genome CP003540, EMBL-EBI Database

http://www.ncbi.nlm.nih.gov/nuccore/CP003540.1

Publications Patents (Total-3)

1. S. S. Nilegaonkar, V. P. Zambare, P. P. Kanekar, P. K. Dhakephalkar, S. S. Sarnaik, N. K. Chandrababu,

Rama Rajaram, B. Ramanaiah, T. Ramasami, Y. K. Saikumari and P. Balaram Indian Patent on ‘A novel

protease for industrial application’ Filing date 16th November, 2006 (Indian Patent NO. 2471DEL2006).

2. S. S. Nilegaonkar, V. P. Zambare, P. P. Kanekar, P. K. Dhakephalkar, S. S. Sarnaik, N. K. Chandrababu,

Rama Rajaram, B. Ramanaiah, T. Ramasami, Y. K. Saikumari and P. Balaram German Patent on ‘A novel

protease for industrial application’ Filing date 23rd March, 2007 (German Patent NO. 102007013950.2.)

3. S. S. Nilegaonkar, V. P. Zambare, P. P. Kanekar, P. K. Dhakephalkar, S. S. Sarnaik, N. K. Chandrababu,

Rama Rajaram, B. Ramanaiah, T. Ramasami, Y. K. Saikumari and P. Balaram ‘A novel protease for

industrial application’ Filing date 31st October, 2007 (US Patent application no. 11/931537, Confirmation

no. 1314).

Publications Popular Articles/ New Reports/ Interview

1. Prairie cordgrass for ethanol production (http://jrse.aip.org/prairie_cordgrass_for_ethanol_produc tion)



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