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ASHWINI SHESHASAYEE

**** ********* ****, #***

Arlington, VA – 22209

202-***-****

abgo1q@r.postjobfree.com

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EDUCATION

Georgetown University, Washington D.C

M.S Biochemistry and Molecular Biology December 2011

Padmashree Dr D.Y Patil University of Biotechnology and Bioinformatics, Mumbai, India

Bachelor of Technolo gy (B.Tech) Biotechnology July 2010

EXPERIENCE

Research Assistant

National Human Genome Research Institute, NIH, Maryland June 2012 to present

Currently working in the Lab of Genetics and Molecular biology

- Studying the patho -physiology of Methy Malonic Acid (MMA) disorder using liver directed

recombinant AAV gene delivery in model organisms

National Cancer Institute, NIH, Maryland January 2012 to May 2012

Worked at the Lab of Receptor Biology and Gene expression

- Investigated the contribution of MR-to-GR dimer interactions to gene regulation in the same

cell type by using co-immunoprecipitation and ChIP experiments.

Research project for Masters degree

June 2011 – December 2011

Georgetown University Medical Center, Washington D.C

Title :“Characterization of eNOS in Breast Cancer”

- Determined the presence of eNOS and nitrite in hormone dependent and hormone

independent breast cancer cell lines by a quantitative and nitrite assay study and using

western blot analysis.

Bioinformatics Project Intern

June 2011 – October 2011

Georgetown University Medical Center, Washington D.C

Worked on a Data Curation project

- Carried out manual curation, structural curation, and text curation of biological data (both

binding and functional assay results) of drug-target associations deposited in the public data

bases such as bindingDB and ChemBL

Research Assistant

Institute of Molecular and Cell Biology (IMCB), A*STAR, Singapore

January 2010 – June 2010

Worked for the Undergraduate thesis project

Title : “New genes discovered in Retrograde traffic siRNA screen”

- Identified 13 new genes by a High throughput RNAi screen, possessing a transmembrane

domain and involved in the retrograde transport pathway which is misused by harmful toxins

causing harmful effects in the cell.

- Examined the specific localization of 13 new genes in the ER and Golgi by preparing

fluorescence protein tagged plasmid vectors by cloning, transform them in bacteria, isolation

of the plasmid, sequencing, lipid based transfection in the HeLa cells, Immunofluorescence

technology and fluorescence imaging.

2

P UBLICATIONS

Sivanesan Dakshanamurthy, Naiem T. Issa, Shahin Assefnia, Ashwini Seshasayee, Oakland J. Peters,

Subha Madhavan, Aykut Uren, Milton L. Brown and Stephen W. Byers

P redicting New Indications for Approved Drugs Using a Proteochemometric Method

J. Med. Chem., 10.1021 (2012)

TECHNICAL SKILLS

Molecular Biology

Construction and cloning of Recombinant DNA (Gateway Invitrogen technology)

Primer designing and Plasmid DNA isolation : Mini, Midi and maxi prep

Protein, DNA and RNA extraction from cells and tissues

SDS PAGE and Agarose gel electrophoresis

Western blot and Southern blot analysis

CoImmunoprecipitation and Chromatin Immunoprecipitation (ChIP)

Protein purification through Ion exchange chromatography

Tissue/Cell Culture

Basic cell culturing : culturing, passaging, freezing and seeding cells on plates

Lipid based Transfection, siRNA transfection and co-transfection

Induction and study of Apoptosis

Instrumentation and Diagnostic skills

qRT PCR

HPLC, UV-vis Spectrophotometer ( Nanodrop)

ELISA

Robotic Liquid handling sampler (Tecan Group Ltd)

Cytogenetics and Microbiological skills

Fluorescent Microscopy and Immunfluorescence technology

Immunohistochemistry and Immunocytochemistry

Standard bacterial plating and liquid cultur e

Bacterial Transformation

Animal work

Basic mice handling (restrain) and euthanizing

Bioinformatics

Curation of protein data and validation, Data mining, Chemiinformatics

Proficient in using various bioinformatics tools and databases

Vector NTI sequencing software and sequencher for DNA analysis, Image J software

Data Analysis using SAS

Proficient in SAS programming that involves data extraction, data processing and quantitative/

qualitative data analysis

Extensive knowledge in using all SAS functions, data steps, procedures, graphs and macros.

Computer skills

MS office : Word, Excel, Powerpoint, Outlook ; Adobe Illustrator, Pivot table, Basic HTML

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