Engineer Assistant

Deep J. Golwala

*** ***** **, # *

Elkton, Virginia-22827

Cell: 619-***-**** ********@***.***

Education:

Graduate School

University of Minnesota, Twin cities

Master of Science in Microbial Engineering, 2009-2012 GPA: 3.52/4

Dissertation Thesis: “Characterization of Cyanobacterial Fatty Aldehyde Deformylase and Biodegradation of

Hydrocarbons by S. oneidensis MR-1”

Undergraduate School

Saurashtra University, Gujarat, India

Bachelor of Engineering in Biotechnology Engineering, 2004-2008 GPA: 4.00/4

Thesis: “Studies on Nitrate Removal by Radiation Resistant Bacteria”

Work / Research Experience: 2.8 years

WORK EXPERIENCE

1) August 2012- Engineer I ( Contractor )

Present Vaccine Technical Operations

Merck & Co, Inc

Fermentation process design for vaccine production.

Experienced with 15 liter tank and shake flask fermentation for vaccine production .

Handling of seed and production tanks using Delta V system.

Working with SIP / CIP systems.

Working in BSL-2 laboratory setting.

DOE using minitab.

Excellent understanding of ultrafiltration and diafiltration.

cGMP training and compliance.

Lean Management and Establishment of inventory system using Kanban system.

2) January 2010 Research Assistant

-2012 Wackett Lab (Protein Chemistry)

University of Minnesota, Twin Cities.

Molecular Biology:

Construction and designing of vectors.

Isolation of plasmid and genomic DNA

Designing of primers.

Gene isolation and PCR.

Competent cell preparation.

Restriction digestion reactions.

Agarose and Acrylamide gel electrophoresis.

Ligation of DNA fragments

Transformation of plasmids

Colony screening.

Site directed mutagenesis.

Cell Culture techniques. ( E.coli, Cyanobacteria, Yeast, D. radiodurans R1, and Paracoccus sp )

Aseptic handling techniques.

Protein Studies:

E.coli shake flask fermentation for protein production (2 liter).

Optimizing protein yield and expression studies.

Analyzing fermentation product using whole cell and biochemical assays.

Protein expression and characterization studies.

Protein purification using affinity chromatography, size exclusion and ion-exchange chromatography

(FPLC-GE Pharmacia).

Western Blot.

SDS-PAGE.

UV-Vis spectroscopy.

Working knowledge of HPLC.

Gas chromatography-mass spectrometry-flame ionization detection. (GC-MS-FID).

In vitro assay development and optimization.

2) January 2010 Student Training Course (Fermentation Studies)

Biotechnology Resource Center

University of Minnesota, Twin Cities

Experience with 10 / 15 liter bioreactors including cell culture, aseptic techniques, sterilization, media

preparation, inoculums preparation, cell harvesting and downstream processing.

Growth studies and yield calculations.

Analyzing fermentation product using whole cell and biochemical assays.

3) September 2009 Research Assistant

Wang Lab (Enzyme Kinetics)

University of Minnesota, Twin cities

Working with small 20 ml reactor for studying enzyme kinetics and continuous monitoring of product using

GC-FID.

Enzyme assay and specific activity calculations.

Kinetic studies and calculations for Km and Vmax.

Achievements :

Undergraduate GPA of 4.0.

Poster presentation at IREE conference “Renewable route to Drop-In Jet Fuel: A New Role for Diiron

Cluster Enzymes”.

Poster presentation in S&T day event at Merck “Lean Approach Towards Inventory Management for Bio -

Safety Level 2 ( BSL-2)– Pilot scale operations – Haemophilus influenzae (Type B ) Fermentation Process”

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