Engineering Engineer

SUMMARY ______

Productive and result oriented chemical engineer seeks challenging position

in fermentation / research setting.

PROFESSIONAL EXPERIENCE ______

Allylix Inc - Lexington, KY 10/10 to

present

Research Associate, Fermentation and Process Development

I Set up and tear-down 2-10L New Brunswick and Sartorious fermenters. I

conduct experiments for process optimization and process improvements. I

also develop methods for cells, nutrient and product quantification. I

create templates for data collection and data analysis. I also run new

yeast strains in fermenters (1-10L) in order to study their metabolism.

Allylix Inc - Lexington, KY 02/09

to 09/10

Research Associate, Strain / Metabolic Engineering

I worked on engineering yeast strains by integrating and knocking out genes

in chromosome. I constructed plasmids that modified the yeast genetic make-

up. I analyzed the engineered strains for metabolite production in shake

flasks. I worked as link between the molecular biology and the fermentation

department of the company. I developed a model to optimize the feed rate of

glucose into the fermenter.

Allylix Inc - Lexington, KY

5/08 to 8/08

Research Intern

I set up micro-fermenters to screen best terpene producing yeast isolates.

In another project I cloned P450 enzyme in an attempt to oxidize terpenes

produced in engineered yeast strains. I was offered a fulltime position at

Allylix Inc even when the company was in hiring freeze

EDUCATION

University Institute of Chemical Technology, Mumbai India BS May 2004

Chemical Engineering Major

University of Minnesota, Minneapolis MS Aug 2006

Microbial Engineering Major GPA 3.63/4.00

University of Iowa, Iowa City M.S Jan

2009

Chemical Engineering Major PhD Candidate

Nov 2007

GPA 3.86/4.00

Research Experience in Graduate School

Dept. of Chemical Engineering, Univ. of Iowa

8/06 - 1/09

Research Advisor: Prof. Mani Subramanian

Caffeine dehydrogenase was purified from Pseudomonas sp. CBB1. A genomic

library of CBB1 was made and screened for caffeine dehydrogenase to obtain

the sequence of the operon.

Biotechnology Institute, Univ. of Minnesota

8/04-8/06

Research Advisor: Prof. Lawrence Wackett

I used a University of Minnesota web-database on biocatalysis and

biodegradation to model a system to predict biodegradation of xenobiotic

compounds (http://umbbd.msi.umn.edu/)

TECHNICAL SKILLS

. Operating New Brunswick and Sartorious 1-10 L fermenters.

. Molecular Biology techniques such as PCR, q-PCR, gel electrophoresis, DNA

cloning into bacteria and yeast.

. Protein purification (Akta FPLC) and characterization.

. Chemical Analysis using HPLC and GC

. Script writing in Perl/SQL, Programming in C

PUBLICATIONS

. Chi Li Yu, Yogesh Kale, Sridhar Gopishetty, Tai Man Louie, and Mani

Subramanian (2008) "A Novel Caffeine Dehydrogenase in Pseudomonas sp.

CBB1 Oxidized Caffeine to Trimethyluric Acid" J. Bacteriology 190(2):772-

6

. Chi Li Yu, Tai Man Louie, Yogesh Kale, Ryan M. Summers, Sridhar

Gopishetty, and Mani Subramanian (2009 ) "Two Distinct Pathways for

Metabolism of Caffeine and Theophylline in Pseudomonas putida CBB5" J.

Bacteriol 191(14):4624-32

. MS thesis

"Refinement of Metabolic Logic for Biodegradation Pathway

Prediction", (2006)

"Purification and Sequencing of Caffeine Dehydrogenase from

Pseudomonas sp. CBB1", (2009)

PRESENTATIONS

. Best Poster Award at 17th Center for Biocatalysis and Bioprocessing, Oct

2008 (CBB) conference " Purification And Sequencing Of Caffeine

Dehydrogenase From Pseudomonas Sp. CBB1" Yogesh Kale, Michael Louie, Chi

Li Yu and Mani Subramanian

. Fourth international congress on biocatalysis, Hamburg, Germany, Sep 2008

"Caffeine to Trimethyluric Acid is Catalyzed by a Novel Quinone-Dependant

Dehydrogenase, NOT and Oxidase" Chi Li Yu, Yogesh Kale, Sridhar

Gopishetty, Tai Man Louie, and Mani Subramanian

. 16th Center for Biocatalysis and Bioprocessing (CBB) conference, Oct

2007 "Bacterial Degradation of Caffeine via Demethylation and Oxidation"

Yogesh Kale, Chi Li Yu, Sridhar Gopishetty and Mani Subramanian

. 16th Center for Biocatalysis and Bioprocessing (CBB) conference, Oct

2007 "Bacterial Degradation of Caffeine via Demethylation and Oxidation"

Yogesh Kale, Chi Li Yu, Sridhar Gopishetty and Mani Subramanian

. 107th American Society of Microbiology (ASM) national meeting, May 2007

Toronto, CA,, "Bacterial Degradation of Caffeine via Demethylation and

Oxidation", Chi Li Yu, Yogesh Kale, Sridhar Gopishetty and Mani

Subramanian

. 231st American Chemical Society (ACS) national meeting, Atlanta, GA, Mar

2006 "Refinement of Metabolic Logic for Biodegradation Pathway

Prediction", Yogesh Kale, Larry Wackett and Lynda Ellis, Biotechnology

Institute, University of Minnesota

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