Assistant Development

ANKIT V. PATEL

**** ****** ***, *** # *, Union city, NJ-07087; Phone: 478-***-****

***********@*****.***

Summary:

. Scientific researcher with expertise in Molecular and Cell

biology, Recombinant Cell line development, Protein expression

and Cell- based assay development

. Ability to work independently and assist peers/co-workers

related to research experiments

. A team player with strong interpersonal, oral and written

communication skill

. Experience in analyzing, troubleshooting and interpreting

experimental methods and results

Research Skills:

Molecular Biology: Nucleic acid isolation/extraction, quantification

and purification from mammalian and microbial cell;

Recombinant DNA cloning; primer design; cDNA library

screening; qRT-PCR; yeast 2 hybrid system; PCR array;

shRNA knock-down

Cell Biology: Mammalian cell culture (Adherent & Suspension), In vitro

drug dosage, transfection, stable cell line

generation, florescent microscopy, cell lysis

techniques, multiwell plate-based proliferation,

apoptosis and cytotoxic assays

Protein Chemistry: Separation of proteins by FPLC, protein

quantification assays, SDS-PAGE, western blot, ELISA,

ImmunoHistochemistry, Immunoprecipitation; histones

purification from mammalian cell

Instrument: BioLogic LP chromatography system, Genetix-Clone select imager,

QIAcube, AB ViiA 7 qPCR, Vi-CELL and Cedex cell

viability analyzer, TECAN Freedom EVO, Advanced Micro-

bioreactor (AMBR-TAP biosystems), YSI biochemistry

analyzer, NanoDrop, Eclipse TE2000 microscope,

Agilent tech. HPLC system

Education:

Master of Science in Molecular and Cell Biology

LONG ISLAND UNIVERSITY, Brooklyn, NY

2011

> Awarded a Teaching Assistant fellowship providing full tuition

and stipend while employed as a Teaching assistant for

undergraduate biology classes

Bachelor of Science in Pharmaceutical Science

RAJIV GHANDHI UNIVERSITY OF HEALTH SCIENCES, Bangalore, India

2007

Experience:

JOHNSON & JOHNSON, Skillman, NJ

May 12- Nov 12

Assistant Scientist (contract position through Kelly scientific)

. Research scientist engaged to investigate mechanism of action of

natural compounds in skin biology using a solid background of

molecular and cellular biology.

. Established in-house high through put screening assays for certain

molecular signaling pathway

. Gene expression study: RNA isolation and Taqman gene expression qPCR

assay

. Lentiviral shRNA gene silencing

MERCK & CO. INC, Union, NJ

Sep 11 - Apr 12 Bioprocess

Developments Co-op

. Participated in upstream Bioprocess development of recombinant

protein production

. Developed scalable, robust and reproducible mammalian cell culture for

therapeutic protein production

. Improved feeding strategies and characterized molecular profile of CHO

cell to enhance recombinant protein

. Studied of post translational modification and N-Glycan pattern

analysis of therapeutic antibody

. Processed of serum free adaptation of SP2/0-Ag14 and CHO host cell

line to optimize protein production

. Analysed of production cell lines by proA and ELISA

. Operated high throughput automated platform for the development of

manufacturing cell line for protein therapeutic

. Collaborated with lab staff in team environment providing assistance

during laboratory experimentation

LONG ISLAND UNIVERSITY, Brooklyn, NY

Sep 09 - Sep 11

Research Assistant

. Employed differentiation therapy on human melanoma cell line (HO-1) to

determine novel drug target

. Screened synthetically synthesized polyphenolic derivatives and

determined structural activity relationship

. Conducted cell proliferation assays to determine viability, cell count

and drug effect

. Oversaw HO-1(melanoma), DU145, LNCaP and HeLa cell line culture,

created lab reagent and media

. Extracted nucleic acid and protein from cancer cell to perform qRT-

PCR, immunoblotting and PCR array

. Performed gene expression analysis on cancer cell line and identified

targeted signaling pathway

. Maintained research report database and presented data with research

results

LONG ISLAND UNIVERSITY, Brooklyn, NY

Sep 09 - May 11

Teaching Assistant

. Biology Department (Subjects: Advanced Microbiology, Human Physiology

and General Biology)

. Prepared microscopic slides, specimens, models, reagent and dissection

material for a class of 30+ students

. Aided students with study material, lab protocols and coursework

LIU MBRS-RISE summer laboratory Workshop- Brooklyn, NY

May-Jun 2010/11

Laboratory/Teaching assistant

. Prepared gene clones through the isolation of RNA from HeLa cell and

implemented RT-PCR

. Demonstrated molecular cloning and basic molecular biology technique

THE HISTOCHEMICAL SOCIETY, Woods Hole, MA

March 23-26, 2010

. Performed 17 hour of laboratory training: antigen retrieval, double

antibody labeling, detection, and florescent microscopy

. Attended 15 hour of lecture: tissue fixation, antibody, florescence

and electron microscopy, amplification methods and imaging

TORRENT PHARMACEUTICAL, India

Apr 07- Apr 08

Research Associate

. Worked in a team of In vivo Pharmacology Laboratory

. Determined maximally tolerable dose (MTD) of a compounds and PK/PD

studies in mice

. Performed IV, IP and SC injections in mice and mouse facility

maintenance

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