Research Associae
Resume:
Trusha Parikh *** E El Camino Real
************@*****.*** Sunnyvale, CA 94087
WORK EXPERIENCE
Research Associate at Genentech
June 2012-present
Drug delivery group
Responsibilities: To conduct in vitro drug release experiments with the
ocular medical device to evaluate it's release performance
-Successfully analyzed the samples using UV-Vis, Bradford, SEC, ELISA as
per GLP guidelines
-Used chemstation and excel to generate and compile data and generated in
vitro release profiles
Associate Scientist I at Corium International
September 2011-June 2012
Analytical R &D department
Responsibilities: To perform stability studies on proteins formulated in
microneedles
-To evaluate protein binding from serum samples and to characterize mAbs as
per GMP guidelines
-Performed buffer extraction and analyzed samples using SDS PAGE, BCA
assay, UV, SEC, IEC and RPHPLC -Successfully
optimized ELISA for protein binding and processed data using softmax and
totalchrom
-Trained employees on SEC, SDS-PAGE and ELISA.
Intern at Genentech
June-August 2011
Hybridoma development in Antibody Engineering department
Responsibilities: To Evaluate of various adjuvants to increase immune
response and to implement in vivo electroporation for antibody development
-Performed Transformation procedures to scale up DNA, maintain cell lines,
performed in vivo immunizations (ID, IP), in vivo electroporation, in vivo
bioimaging, ELISA and FACS
-Successfully generated data using Flowjo, M3vision and compiled data using
prism
Intern at Genentech
June-August 2010
Hybridoma development in Antibody Engineering department
Project involved comparative study on DNA immunization using HTV delivery,
liposomal HTV delivery and intra dermal injection with in vivo
electroporation to increase efficiency of current HTV immunization.
-Performed DNA immunizations and in vivo bioluminescence imaging for
protein expression at various time points, FACS analysis to detect mAb
titer
-Successfully generated data using M3vision and FACS analysis
SKILLS AND TECHNIQUES
Analytical chemistry, molecular and cell biology, immunology
|-Tissue culture |-Protein |-Isolation/purification of |
|-SDS-PAGE |extraction/purification |plasmid |
|-Immunocytochemistry |-ELISA |-PCR |
|-In vivo/vitro bioimaging |-Western blot |-Agarose gel Electrophoresis |
|-Transfection (Transient) |-DNA scale up |-Hybridoma production for mAb|
| |-Bradford/BCA assay | |
| |-HPLC (SEC, RP, IEC) |-FACS |
| | |-Immunization: IP, IV, SC, ID|
| | | |
EDUCATION
San Jose State University, Masters in Biological sciences (GPA 3.775)
May 2011
Classes studied: 1) Immunological techniques (Lab includes mice
immunization, production of hybridoma secreting monoclonal antibodies,
further screening and cloning, purification and characterization of mAb),
2) Bioinformatics,
3) Seminar in Molecular Biology and Microbiology, 4) Immunology class &
Lab, 5) Molecular and cell biology Lab, 6) Graduate studies in Biology, 7)
Flow Cytometry 8) Virology
M.Sc. Pharmacology and Biotechnology
January 2007
Sheffield Hallam University (United Kingdom)
Classes studied: DNA technology, Cell biology, Biology of Infectious
Disease, Molecular pharmacology, Molecular biotechnology and Research
methods and dissertation on DNA vaccines and tuberculosis
B.Sc. Microbiology
July 2005 M. G. Science College, Gujarat
University (India)
PROJECTS SUMMARY
Dr. Tzvia Abramson's lab at SJSU
October 2009
Experiment on Immunomodulation by Bordetella species in whooping cough
disease.
Research project at Sheffield Hallam University under Dr. Rowena Bunning
June-August 2006
Studies on production of ADAMTS 9 by Human Neuroblastoma cells (SHSY5Y)
POSTER PRESENTATION
-Presented poster titled "DNA immunization: Evaulation of hydrodynamic tail
vein (HTV) delivery, liposomal HTV delivery and intra dermal in vivo
electroporation" at the 2010 and 2011 Genentech Intern poster symposium
-Presented poster on HIV at Sheffield Hallam University annual poster
event: Was awarded 1st prize
COMPUTER SKILLS
-Efficiently use Microsoft word, excel and powerpoint
-Familiar with R language for statistical analysis
COMMUNICATION SKILLS
-Excellent verbal and written communication skills:
Have written SOP and gave project presentations
EXTRACURRICULAR ACTIVITIES
-Volunteered at a Medical Health awareness camp at Bhuj, India, 2008
-Volunteered at the International Special Olympics, Ahmedabad, India,
December 2004
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