Assistant C

Santosh G. Valeja

National High Magnetic Field Laboratory

**** * **** ***** *****

Tallahassee, FL-32310

*************@*****.***

OBJECTIVE

To secure an analytical scientist position with emphasis on separation

science, mass spectrometry, wet chemistry and analytical techniques.

EDUCATION

Ph.D. (Expected Spring 2013) Fall 2007 - Present

Florida State University, Tallahassee, FL

Major: Chemistry

Division: Bioanalytical Chemistry (GPA: 3.64)

Bachelor of Technology Fall 2003- Summer 2007

University Institute of Chemical Technology, Mumbai, India

Major: Chemistry (Intermediates and Dyes formulation)

Rank: First Class with Distinction (81%)

PROFESSIONAL EXPERIENCE

Graduate Research Assistant Spring 2009 - Present

National High Magnetic Field Laboratory, Florida State University,

Tallahassee, FL

Ion Cyclotron Resonance Mass Spectrometry Program

Advisor: Dr. Alan G. Marshall, Director of ICR Program

Research Area: Hydrogen/deuterium Exchange Monitored by HPLC ESI FT-ICR

Mass Spectrometry and Top-Down MS: Method development, optimization and

applications.

. Designed chromatographic method to reduce back-exchange of D for H in

H/D exchange MS by suitable choice of HPLC separation modifiers.

. Developed new method for improved chromatographic separation, charge

enhancement and sensitive detection for intact proteins.

. Performed baseline resolution (new benchmark) and structural analysis

of therapeutic monoclonal antibody on FT-ICR MS by optimizing numerous

instrument conditions.

. Evaluated the effects of heavy gases (nitrogen, argon, xenon) for

fragmentation of intact proteins with beam-type collision activated

dissociation.

. Conducted top-down mass spectrometry for different "supercharged"

intact proteins with collision activation and electron capture

dissociation.

. Worked on HeLa cells to probe dynamic changes in PTM's between

asynchronous and M-phase arrested HeLa cells.

. Researched top-down mass spectrometry of intact proteins greater than

50 kDa.

Graduate Teaching Assistant Fall 2007 - Fall 2008

Department of Chemistry and Biochemistry, Florida State University,

Tallahassee, FL

. Conducted general chemistry lab (CHM1046L) and introduction to

analytical lab (CHM3120L) for 30 students each during summer and fall

2008.

. Worked as an NMR Technician on Bruker 300 MHz analyzing organic

chemistry lab samples for undergraduates during Fall 2007 and Spring

2008.

Undergraduate Research Assistant Fall 2006 - Spring 2007

University Institute of Chemical Technology, Mumbai, India

. Worked on "Synthesis of 2-(4'-Aminophenyl)-5-AminoBenzimidazole",

under the guidance of Prof. V. R. Kanetkar and Dr. N. Sekar.

This work included reviewing of all the possible methods of synthesis,

selecting of the most appropriate method and subsequently synthesizing

the intermediate in the laboratory with the best possible yield and

purity.

Industrial Work Experience May 2006 - June 2006

Sohan Dye chem Pvt. Ltd

. Completed In-Plant Training from May 15 - June 30, 2006. During this

period, I worked in the research and development laboratory to

synthesize different kinds of azo pigments. I also studied large-

scale manufacture.

SKILLS & EXPERTISE

Electrospray Ionization, Mass Spectrometry (9.4 T Q-FTICR MS & 14.5 T LTQ-

FTICR MS, TOF, Ion Trap), High Performance Liquid Chromatography (Jasco,

Shimadzu and Exigent nano-LC system), LC-MS/MS, Hydrogen/Deuterium

Exchange, Protein Characterization, Supercritical Fluid Chromatography

(Jasco), UV-Vis, Gas Chromatography, High Performance Thin Layer

Chromatography, Gel Electrophoresis, Biochemistry.

PUBLICATIONS

1. Valeja, S. G.; Emmett, M. R. and Marshall, A. G., Polar Aprotic

Modifiers for Chromatographic Separation, and Back-Exchange Reduction

for Protein Hydrogen/Deuterium Exchange Monitored by Fourier Transform

Ion Cyclotron Resonance Mass Spectrometry J. Am. Soc. Mass Spectrom.,

2012, 23, 699-707 (2 Citations).

2. Valeja, S. G.; Kaiser, N. K.; Xian, F.; Hendrickson, C. L.; Rouse, J.

C. and Marshall, A. G. Unit Mass Baseline Resolution for an Intact 148

kDa Therapeutic Monoclonal Antibody by FT-ICR Mass Spectrometry Anal.

Chem., 2011, 83, 8391-8395 (9 Citations).

3. Valeja, S. G; Tipton, J. D.; Emmett, M. R. and Marshall, A. G., New

Reagents for Enhanced Liquid Chromatographic Separation and Charging

of Intact Protein Ions for Electrospray Ionization Mass Spectrometry

Anal. Chem., 2010, 82, 7515-7519 (15 Citations).

4. Xian, F.; Valeja, S. G.; Hendrickson, C. L.; and Marshall, A. G.

Artifacts Induced by Selective Blanking of Time-Domain Data in Fourier

Transform Mass Spectrometry (submitted to J. Am. Soc. Mass Spectrom.).

5. Mao, Y; Valeja, S. G.; Hendrickson, C. L.; Rouse, J. C. and Marshall,

A. G. Top-down Structural Analysis of an Intact Monoclonal Antibody by

Electron Capture Dissociation Fourier Transform Ion Cyclotron

Resonance Mass Spectrometry (submitted to Anal. Chem.).

6. Valeja, S. G.; Hendrickson, C. L. and Marshall A. G. Effects of Heavy

Gases on Beam-type Collision Activated Dissociation of Higher

Molecular Weight Proteins (In preparation).

7. Valeja, S. G; and Marshall, A. G. Collisional Activated Dissociation

and Electron Capture Dissociation of Supercharged Intact Proteins with

FT-ICR Mass Spectrometry (In preparation).

8. Valeja, S. G; and Marshall, A. G. Top-down Mass Spectrometry of Intact

Proteins Greater than 50 kDa (Review Paper: In Preparation).

PRESENTATIONS

1. Marshall, A. G.; Bou-Assaf, G. M.; He, H.; Mao, Y.; Valeja, S. G.;

Zhang, Q. and Hendrickson, C. L., H/D Exchange for Mapping Contact

Surfaces in Large Protein Complexes, Amer. Soc. For Mass Spectrometry

Sanibel Conference: Mass Spectrometry Technologies for Structural

Biology, St. Pete Beach, FL, January 19-22, 2012.

2. Mao, Y.; Valeja, S. G.; Rouse, J. C.; Hendrickson, C. L. and Marshall,

A. G., Top-down Structural Analysis of an Intact Monoclonal Antibody by

Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance

Mass Spectrometry, 60th Amer. Soc. Mass Spectrometry Conf. on Mass

Spectrometry & Allied Topics, Vancouver, British Columbia, Canada, May

20-24, 2012.

3. Marshall, A. G.; Huan, H.; Mao, Y.; Valeja, S. G.; Zhang, Q.;

Hendrickson, C. L.; McKenna, A. M. and Rodgers, R. P., FT-ICR MS:

Directions for Future Applications, 10th European FTMS Workshop,

University of Warwick, Warwick, England, April 1-5, 2012.

4. Valeja, S. G.; Kaiser, N. K.; Xian, F.; Emmett, M. R.; Hendrickson, C.

L.; Rouse, J. C. and Marshall, A. G., Unit Mass Resolution for a 148

kDa Intact Therapeutic Monoclonal Antibody by FT-ICR Mass Spectrometry,

59th Amer. Soc. Mass Spectrometry Conf. On Mass Spectrometry & Allied

Topics, Denver, CO, June 4-9, 2011.

5. Valeja, S. G.; Kaiser, N. K.; Xian, F.; Emmett, M. R.; Hendrickson, C.

L.; Rouse, J. C. and Marshall, A. G., Unit Mass Resolution for an

Intact 148 kDa Therapeutic Monoclonal Antibody by FT-ICR Mass

Spectrometry, 8th N. Amer. Fourier Transform Mass Spectrometry Conf.,

Key West, FL, May 1-5, 2011.

6. Marshall, A. G.; Blakney, G. T.; Bou-Assaf, G. M.; Emmett, M. R.;

Hendrickson, C. L.; Valeja, S. G; Zhang, H.-M. and Zhang, Q., Mapping

Contact Surfaces in Protein Complexes by Solution-Phase H/D Exchange

Monitored by Ultrahigh Resolution FT-ICR Mass Spectrometry, FACSS 2010,

Raleigh, NC, October 17-21, 2010.

7. Valeja, S. G.; Tipton, J. D.; Emmett, M. R. and Marshall, A. G., New

Reagents for Enhanced Charging of Intact Protein Ions for Liquid

Chromatography Tandem Mass Spectrometry, 58th ASMS Conf. on Mass

Spectrometry & Allied Topics, Salt Lake City, UT, May 23-27, 2010.

8. Hendrickson, C. L.; Blakney, G. T.; Bou-Assaf, G. M.; Zhang, H.-M.;

Tipton, J. D.; Valeja, S. G; Emmett, M. R. and Marshall, A. G.,

Improved FT-ICR MS of Biomolecules, 9th European FTMS Workshop,

Lausanne, Switzerland, April 6-9, 2010.

9. Valeja, S. G.; Emmett, M. R. and Marshall, A. G., Reduced Back-Exchange

by Modified RPLC Mobile Phase Separation of Proteolytic Peptides for MS

Analysis of H/D Exchange of Solution-Phase Proteins, 57th Amer. Soc.

Mass Spectrom. Annual Conf. on Mass Spectrometry & Allied Topics,

Philadelphia, PA, May 31-June 5, 2009.

10. Valeja, S. G.; Emmett, M. R. and Marshall, A. G., Reduced Back-Exchange

by Modified RPLC Mobile Phase Separation of Proteolytic Peptides for MS

Analysis of H/D Exchange of Solution-Phase Proteins, 7th North American

FT MS Conf., Key West, FL, April 19-23, 2009.

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