Technician Engineer
Resume:
LEE R. BUSHEN
Niskayuna, NY 12309
abgfxg@r.postjobfree.com
Health, Safety and Environmental Coordinator, Kenny Construction 10/2012-Present
Indio, CA
• Perform and document safety and environmental audits using Safetynet software on an iPad
• Suggest corrective environmental and safety measures to supervisors in order to adhere to state,
and federal OSHA and EPA requirements
• Present an hour long safety orientation to new employees two times each week
• Conduct accident investigations
• Prepare Apparent Cause Evaluation (ACE) reports and safety alerts
U.S. Army, Various Locations 11/2004 – 6/2012
Engineer Officer
• Lead route clearance platoon on 100+ successful missions with zero casualties through the hostile
environment of southern Afghanistan
• Advised the Operation commanders on threats of Improvised Explosive Devices (IEDs) in the
area of operation
• Wrote technical reports detailing the events of each mission
• Created and complied PowerPoint presentations within a strict timeline
Satellite Communication Operator/Maintainer
• Trained as a Satellite Communications Operator/Maintainer at the Signal School in Fort Gordon,
GA
• Ensured all training was compliant with environmental regulations
• Excelled as a company executive officer, a commissioned officer’s position, while holding the
rank of specialist
• Holds a current secret security clearance
Research Technician, Cornell University 6/2002 – 12/2003
Ithaca, NY
• Purified RNA from animal tissues using aseptic techniques
• Triaged and catalogued species of animal samples to determine priority of testing
• Tested up to 50 samples daily during high volume input
• Developed RT-PCR protocol to test for West Nile Virus in RNA samples
• Maintained mammalian cell culture lines
• Tested animal blood and serum for West Nile Virus using ELISA
• Recorded test results into VETLIMS database
• Designed primers for PCR of Eastern Equine Encephalitis
• Assisted in performing plaque reduction assays in Biosafety Level 3 laboratory
Research Technician, Binghamton University 3/2001 – 6/2002
Binghamton, NY
• Performed numerous molecular biology laboratory techniques including: PCR, Cloning, DNA
and RNA purification, Western Blots, Southern Blots, and DNA sequencing
• Ran agarose gel electrophoresis and PAGE
• Supervised and trained undergraduate students on numerous laboratory techniques and protocols
• Performed primary neuronal cell cultures
• Co-instructor of the molecular biology section of a graduate level Biomedical Anthropology
course with laboratory concentration
• Developed method for the isolation of total RNA from frozen human brain tissue while working
in a Biosafety level 2 laboratory
• Responsible for the care and maintenance of laboratory equipment and ordering of supplies
through the university system
Research Technician, Proctor and Gamble Pharmaceuticals 11/2000 – 3/2001
Norwich, NY
• Measured tablet coating thickness using Philips XL30 ESEM scanning electron microscope
• Elucidated the size of powered medicine particles using LA-920 Laser Light Scattering Particle
Sizer
• Input test results into LIMS database
• Performed light microscopy with Image-Pro Plus imaging software
Student Researcher, Western Kentucky University 1/2000 – 5/2000
Bowling Green, KY
• Constructed a plasmid vector to study the regulation of transcription of the bile acid inducible
promoter in Eubacteria 12708
• Proficiently performed cloning, plasmid preps, transfections, ligations, restriction digests,
bacterial culture, gel electrophoresis, PCR and DNA sequencing
• Presented research at Sigma Xi conference
Certifications and Skills
OSHA 30
MS Word, Excel, PowerPoint and Outlook
Apple and PC computers
Safety Net
Secret Security Clearance
Associations and Honors
Tri-beta honors society member
Association of Undergraduate Geneticists (AUG) member– academic club for students in the recombinant
genetics program
Education
May 2000 - Bachelors of Science in Biology with concentration in Recombinant Genetics
Western Kentucky University, Bowling Green, KY
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