Arpitha Banaji

**** ********* ******, ** ***# **, Washington, D.C. 20007 abg4nr@r.postjobfree.com 202-***-****

Education

Dec 2014

Georgetown University, Washington D.C

Masters in Microbiology and Immunology

PES Institute of Technology, Bangalore, India

Jul 2013

Bachelor of Engineering in Biotechnology

Skill Set

Laboratory Skills

PCR, RT PCR, Primer Design, PCR Product Purification, Agarose Gel Electrophoresis, Gel Extraction

ELISA, Western Blot, Flow Cytometry, Immunostaining, In-Situ Hybridization, ISH Probe Synthesis

Cell Culture, TALI Apoptosis, Maxi Prep, Bacterial Transformation, Restriction Digestion, Basic Microb iology Lab

Techniques

RNA Extraction, DNA Extraction, cDNA Library Preparation, Viral Presence

Dissection of E8.5 Embryo, SEM Embryo Preparation, Photographing Embryos, Making Photo Plates

Computer Skills

Adobe Photoshop CS6, Microsoft Office Suite

Professional Experience

Jun 2014 –

Memorial Sloan Kettering Cancer Center, New York City, NY

Aug 2014

Developmental Biology Intern - Bmp2 expression and its role in ventral folding morphogenesis

Genotyped mouse lines (designed PCR protocol & was successful in troubleshooting PCR), designed primer, isolated

DNA from adult and embryonic tissue, purified PCR product for sequencing and Immunostained cells grown on

cover slips

Performed Whole mount RNA in-situ hybridization (WISH), RNA Probe synthesis, Bacterial Transformatio n, Maxi

Prep, Gel Extraction, SEM Embryo Preparation, Restriction Digestion, Photographed embryos and handled mouse

post fixation

Observational : Setting up mice for breeding, Estrous selection, Checking plugs, Weaning litters an d toe clipping for

genotyping, Colony maintenance, Dissection of E7.5 and E8.5 embryos

Virology Laboratory, Georgetown University, Washington, DC

Sept 2013 –

Student Research Assistant

May 2014

Performed drug testing for Hepatitis B Virus (HBV) in a Woodchuck animal model – NIH and ROCHE funded

projects

Extracted RNA, DNA, prepared cDNA library, RT PCR and checked the viral presence post treatment with the drug

Stempeutics India Pvt Ltd., Bangalore, India

Project Intern - Mesenchymal Stem Cells and Evaluation of Their Potency in Treatment of Liver Cirrhosis

Performed a literature review on various articles for the ongoing animal model studies and clinical trials on stem cell Jan 2013 –

therapy for liver cirrhosis May 2013

Expanded colony of MSCs, characterized MSCs using Flow Cytometry, differentiated MSCs into Adipocyte,

Chondrocyte and Osteocytes

Conducted population doubling kinetics, checked cell viability using TALI Apoptosis machine, performed CFU-F

Assay, HGF ELISA and quantification of the cells

Micro Labs Limited, Bangalore, India

Quality control intern

Gathered company's quality level requirements and regulations set by Pharmacopeia

Jun 2011

Prepared various media under aseptic conditions and cultured variety of microorganisms, a nalyzed water samples

collected from different stages of the drug manufacturing process for microbial growth and interpreted the results,

analyzed microbial growth of various drug samples obtained in powder and capsule form

Achievements

Conducted Paper Literature Reviews on “Biological accumulation as a tool to recover heavy metals from the soil”

and “Food applications of electronic nose"

Secured distinction awards for 4 consecutive years during undergraduate

Received award for work done during internship at Micro Labs

Elected as Student department Coordinator of Bio Technology Department of my undergraduate university

Elected as a Team Leader by the I-Care group, a State level Community Service Club

References published upon request

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