Manager Supervisor
Resume:
Dear Madam or Sir:
Having had a BS in chemistry, I completed my Master's studies in the department
of Biochemistry faculty of medicine, at the University of Ottawa, Canada during the
summer of 1999. I developed and characterized isoform-specific monoclonal antibodies
against Apo-lipoprotein E (Apo E) one of which differed by only a single amino acid
difference. Other techniques included; protein purification, tissue culture, animal work,
isolation/purification/ quantitation of RNA from tissue and cells, Luciferase assay, RT,Q-
PCR, western blot, HPLC, genotyping, transfection, intracellular staining, ELISA, drug
dose response curve study, proliferation, cytotoxicity, immunfluorescence staining,
immunohistochemistry, and other immunoassays. I can elaborate on these should be
needed.
Since my graduation I have worked as a research assistant in immunochemistry
and analytical chemistry fields in companies as well as in academia. My projects involved
the method development in immunochemistry. I experienced Good Laboratory Practices
(GLP). More recently I was managing manager a lab and been involved in some molecular
biology work including isolation/purification of RNA, performing Q-PCR, RT, genotyping,
transfection, and tissue culture at the surgery department at the University of Miami.
I have worked both in groups and when needed independently. I am a self motivated and
punctual individual who pays careful attention to detail. I enjoy troubleshooting and helping
design experiments.
Cordially,
Tara Ejtehadian M.S.
Curriculum Vitae
Tara Ejtehadian M.S.
8208 SW 172 Terrace, Miami, FL, 33157
Email: abg4kv@r.postjobfree.com or abg4kv@r.postjobfree.com
Education:
June 1999: M.S. Biochemistry, University of Ottawa, Canada. Faculty of Medicine
During my M.S. program, after purification and preparation of
antigens, I developed new isoform specific anti-ApoE monoclonal
antibodies that could be used as probes to study ApoE structure
and function. These mAbs can be used as reagents for an antibody-
based method for rapid determination of ApoE genotype and as
reagents for immunohistology to study the role of individual ApoE
isoforms in disease etiology.
Supervisor: Dr. Ross Milne, Email; abg4kv@r.postjobfree.com
Feb. 1991: B.S. Chemistry, Department of Chemistry, University of Tehran, Iran. GPA
of 89 out of 100.
Publications/presentations:
• Plasma lipoproteins and their receptors. Robert Raffaï, Xingyu Wang, Vinita
Chauhan, Tahereh Ejtehadian, Jesse Bertinato and Ross Milne. Laboratory of
Immunochemistry, University of Ottawa Heart Institute, Ottawa, Ontario, Canada.
INTERSCIENTIA 1(1)- 1996, (Publication date : June 1996).
http://www.uottawa.ca/publications/interscientia/inter.1/milne.html
• Generation of anti-human apolipoprotein E isoform-specific monoclonal antibodies.
Tahereh Ejtehadian, Department of Biochemistry, Faculty of Medicine, National
Library of Canada Document number: 612209717, 1997.
http://www.collectionscanada.gc.ca/obj/s4/f2/dsk2/ftp04/mq20971.pdf
Professional Experience:
Jun 2010-Jan 2011
DeWitt Daughtry Family Department of Surgery, University of Miami, Miami, FL
Summary: As a research associate II; my research was in the area of biochemistry
and molecular biology. I was working on the Hedgehog pathway. The Hedgehog
signaling pathway plays a key role in the control of cell differentiation, growth, and
proliferation. The techniques currently used; isolation/purification of RNA from tissue and
cells, Western blot, Luciferase assay, RT,Q-PCR, dose response curve study, time course
study of 3T3 and Light 2 cells treated with SAG in combination of ATO drugs (agonist and
antagonist), transfection, tissue culture, genotyping, protein purification, and agarose gel
electrophoresis. I was the lab manager and I was involved in laboratory management and
also in charge of ordering/receiving of a large lab including of 10 people.
Dec 2009- April 2010
Department of dermatology and Cutaneous Surgery, University of Miami, Miami, FL, USA
Summary: As a research associate I, Studied the chronic and acute wounds,
Techniques/responsibilities: H and E staining of frozen sections, Cryosectioning,
Immunostaining, isolation of mi-RNA and protein from skin tissue using mirVana PARIS kit,
tissue processing, capturing images and making power point presentation, and some
laboratory management such as ordering/receiving.
Mar. 2008-Nov. 2009
The Miami Institute for Human Genomics, University of Miami, Miami, FL
Summary: As a research associate II; my research was in the area of biochemistry and
molecular biology. I was working on a project related to the Alu elements-the largest family
of mobile elements in the human genome. Alu elements are retrotransposones and they
cause mutagenesis. Their insertion in the human genome sometimes are disruptive and
can result in inherited disorders like breast cancer, hemophilia, familial
hypercholesterolemia, diabetes, and so on. I was involeved in techniques such as
isolation/purification of RNA, PCR, RT-PCR, agarose gel electrophoresis, cDNA extension,
transfection, bacterial culture, cloning, DNA extraction, measuring DNA concentration by
nanodrop spectrophotometer, and some laboratory management such as
ordering/receiving.
Oct. 2004-June 2007
ImmunoVaccine Technologies Inc. with collaboration with Dalhousie University,
Halifax, Nova Scotia, Canada
Summary: In IVT, I was analyzing helical chirality at high precision. Using Varian HPLC
410, and the Aurora program, I was in charge of separating and preparing chiral
molecules. These chiral compounds are therapeutic in curing cancer. Other techniques
used: immunostaining of CD4 and CD8, cell culture of B16 myeloma cells, ELISA, protein
purification (column chromatography),HPLC, protein concentration, protein quantitation,. I
was the lab manager and was in charge of other responsibilities including ordering,
receiving, chemical handling, and solvent purifying.
Supervisor: Dr. Pirouz Daftarian, abg4kv@r.postjobfree.com
Dec. 2002- August 2004
BC Research Institute for Women and Childrens Health, Division of
Gastroenterology, UBC, Vancouver, BC, Canada
Evaluation of immunostimulatory activity of CPG ODN to break tolerance; animal model for
autoimmune liver disease; animal model for primary sclerosing cholangitis (PSC) and
colitis; I performed primary cell culture, immunohistochemistry of frozen and paraffin
embedded sections, in vitro stimulation, T cell proliferation, non-radioactive cytotoxicity,
ELISA, intracellular staining of cytokines, immuneflurescent, isolation and purification of
extra hepatic bile duct cells, minor animal surgery, and flow cytometry. I was the lab
manager and in charge of all the issues related to the lab for example: the operation and
maintenance of laboratory equipment, preparing solutions, reagents, and samples. I was
also involved with writing SOPs.
Supervisor: Dr. Richard Schreiber, abg4kv@r.postjobfree.com
Feb. 2002- Nov. 2002
Northern Lipids Inc. Jack Bell Research Centre, Vancouver, BC
This company was responsible for analyzing different samples received from
pharmaceutical companies making liposome based drugs. Here I was involved in method
development and analysis of drugs encapsulated in liposome. The techniques and
responsibilities of this position included HPLC (Waters) based on GLP criteria, TLC, and
packaging lipids for sale.
Supervisor: Dr. Tom Redelmier, CEO
Jun.1999 - Nov. 2001 Immune Network Ltd., Vancouver, BC
As a technician I, I was in charge of optimizing ELISA, cell culturing, processing human
whole blood and working with serum, maintenance of hybridoma, purification of antibodies,
ordering and receiving supplies, entering data, operating and maintenance of laboratory
equipment, preparing solutions, reagents, and samples.
Supervisor: Dr. Brian Conway, abg4kv@r.postjobfree.com
Oct.1998- May.1999
Department of Pharmaceutical Sciences, UBC, Vancouver, BC
As a research associate I, I studied the interaction of drugs with different lipoprotein
fractions by ultra centrifugation and HPLC. I was also processing human whole blood and
working with human serum.
Skills
• RT and Q-PCR
• Luciferase assay
• Dose response curve study
• Genotyping
• Transfection
• Drug assay
• RNA isolation/purification
• Immunohistochemistry techniques: frozen section
• Intracellular staining
• Ex vivo stimulation
• ELISA
• Immunofuorescent staining of bile duct cells
• Cell proliferation and non-radioactive cytotoxicity assays plus blast
formation and in-vitro stimulation of T cells
• Immunoglobulin Enzymatic digestion
• Radiolabeling of antibodies
• Radioimmunoassay and Radioisotope protocols
• Cell culture
• Animal work and surgery
• Analytical Chemistry
• HPLC
• Protein concentration assays
• Immunoaffinity column chromatography
• Generation, characterization, and Purification of monoclonal antibodies
• SDS PAGE and western blot analysis
Safety:
•Ottawa University, Radioactive Safety and Methodology license
•UBC Radioactive Safety and Methodology license
•UBC Health and Safety-General Laboratory Safety Training
•Dalhousie University, Health and Safety-General Laboratory Safety Training
•University of Miami, General Laboratory Safety Training
Supervisory:
Worked as a teaching assistant at the University of Ottawa and supervised junior students,
and summer student training at the Universities of British Columbia.
References:
David J Robbins, Ph. D
Associate professor
DeWitt Daughtry family department of Surgery
Miller School of Medicine
305-***-**** (office)
1042 RMSB, R104
abg4kv@r.postjobfree.com
Dr Dale J. Hedges, Ph.D, Assistant Professor
Miami Institute for Human Genomics,
University of Miami
Building A, room #112
12500 SW, 152nd Street
Miami, FL, 33177
Tel: 305-***-****
Email: abg4kv@r.postjobfree.com
Dr. Pirouz Daftarian, Ph.D.
Scientist in IVT and adjoins professor at Dalhousie University
BioScience Enterprise Centre
1721 Lower Water Street
Halifax, NS, B3J 1S5
Tel :305-***-****
Email: abg4kv@r.postjobfree.com
Dr. Ross Milne, Ph.D.
Professor, Department of Biochemistry
Faculty of Medicine, University of Ottawa
Tel: 613-***-****
Email: abg4kv@r.postjobfree.com
Dr. Richard A. Schreiber M.D. FRCP(C)
Clinical Associate Professor, University of British Columbia,
Division of Pediatric Gastroenterology, Children's and Women's Hospital
Room K4-2004480, Oak Street
Tel: 604-***-****
E-mail: abg4kv@r.postjobfree.com
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