Medical Assistant

R ESU M E

H I RAL OZA, BS, MS

Cellular No: 201-***-****

abg2xl@r.postjobfree.com

Objective: To work as Research Associate or Research Scientist in the field of

B iotechnology, Biochemistry or related fields in Biology that can effectively utilize my

l aboratory skills and knowledge.

ED UCAT ION

• Master of Science in Medical Biology: Immunology

Long Island University C. W. POST. NEW YORK

May 2012

• Post Graduate in Biochemistry and Bachelor of Science in Biotechnology

Sardar Patel University, India.

J une 2008

(Degree equivalent to U.S. Bachelor’s Degree in Biology and Teaching of Biology

and Chemistry at secondary level; evaluation performed by World Education

Services (WES), New York, NY)

EXPER I ENCE AND RELEVANT SK I L LS

Experience and lab skills in immunochemistry:

• Immuno-histochemistry

• SDS-polyacrylamide gel electrophoresis

• Western blotting

• Expression and Purification of antibody

• Protein precipitation by TCA

• Immunoblot assay

• Radioimmunoassay

• Protein purification including affinity chromatography and gel fil tration

• SDS-Gel Coomassie staining and Silver staining

• In-Vit ro Kinase Assay

Experience and lab skills in molecular biology:

• Counting and Determining the Viability of Cultured Cells

• Flow Cytometry

• Harvesting Cells

• Maintaining Cell L ines

• Cells Transfection

• EL ISA

• PCR

• RT-PCR cloning

• RNA isolation and purification

• Nuclei isolation and nuclear extract p reparation

• Isolation and purification of phage, plasmid, and genomic DNA

• Gene amplification

• Agarose and acrylamide gel electrophoresis, elution of DNA fragment from gel

• Competent cell preparation

• Restriction mapping of DNA

• Recombinant DNA cloning

• Aseptic Techniques

Experience and lab skills in M icrobiology:

• Gram Staining

• Diagnostic test for estimation of Blood Glucose, Urea, Creatinine, Protein and

L ipids

• Blood Group test

• Urine analysis

PROFESSIONAL EXPER I E NCE

• Biologist at Eli Lilly and Company ( Advanced Testing

L aboratory) Indianapolis, I N May 2012 –

P resent

Being part of Diagnostic and Experimental Pathology Group performed

Aseptic Techniques, Cell culture Techniques, Immunohistochemistry ( Leica

Bond I I I, Dako Stainer and Biocare Stainer), Western Blotting, RT-PCR,

s iRNA Knockdown, Maintenance of Auto Stainer, Preparation of media,

M aintenance of Ni t rogen Cabinet, Maintenance of Ni t rogen Tank, H istotech

work, Embed the large and small t issue, Cut the Large t issue, Process the

t issue.

Selection of Monoclonal and polyclonal Antibodies for specific Bio-maker,

Screening them by testing on different Tissue and cell samples, Sorting by

H igh expressing and low expressing antibody, Optimizing the Assay by

selecting best Antigen Retrieval system and Working concentration of

antibody, Screening specific TMA-custom Arrays, Scoring of TMA-array,

P roceed for Validation

Worked on various projects like TIE-2, FGFR3, TrkA, Ang-2 and Mnk-1

• I n te rn Research Assistant a t the S unny Down State Medical Center, NY–

September 2010 to December 2011

Performed Cell Culture Techniques, SDS- PAGE, Protein precipitation,

P rotein purification, SDS-Gel Coomassie Staining, Kinase Assay, Western

B lotting, Protein Chromatography, HPLC

Experience working with antibodies like αCTD, αGdown1, αSpt5, αSpt4,

α E LL1, αP-TEFb, αAff4, αAff1, α NELF, αTat-SF1, αTFI I H, αTFI IE, αTFI ID

and α NF-kB

Determining Role of Human Cellular Factor Gdown1p50-containing RNA

Polymerase I I Complex in H IV-1 Transcription Regulation

• Lab Assistant a t B hailal Amin Hospital, I N D IA June 2008 -

D ecember 2008

Performed diagnostic tests for blood glucose, urea, creatinine, protein and

l ipids on human samples from a hospital.

Performed staining protocols, aerobic culture and blood culture of microbes

for antibiotic sensitivi ty test.

• Lab Assistant a t M . B. Patel Science College, I N D IA June

2007 – May 2008

Performed PCR, RNA isolation, EL ISA, HPLC, Gene amplification, Western

B lotting, Agarose gel Electrophoresis, RT-PCR, Enzyme purification, DNA

cloning, Cell Culture and Aseptic Techniques.

Coursework

Cancer Research, Diagnosis techniques in Molecular pathology, Medical

M icrobiology, Bioinformatics, Pathophysiology I, Pathophysiology I I, Medical

Chemistry, Immunology, Advanced Immunology, Medical Virology and Research

Methods, Microbiology, Biotechnology, Biochemistry, Botany, Zoology, Organic

and Inorganic Chemistry.

M ISCEL LANEOUS

Pursued Master’s thesis under Dr. Camilo Parada, PhD and an Associate

P rofessor in Cell Biology Department in Suny Downstate Medical School,

B rooklyn, NY

P UBL ICAT IO NS

Poster presentation on “ Role of H uman Cellular Factor Gdown1p50-

containing R NA Polymerase I I Complex in H IV-1 T ranscription

R egulation” i n COLD SPRING HARBOUR LAB on AUGUST- SEPETEMBER

2011

H I G H L I G H TS OF QUAL I F ICAT ION

• Master’s degree: M.S. degree in Medical Biology with specialization in

I mmunology

• Broadly t rained and experienced with more than 2 years of professional research,

teaching, managing, and technical experience

• Good working knowledge and experience in different methods and technologies

i n molecular genetics, immunochemistry, Biochemistry, cloning, protein

expression and purification, single gene- and genome-based expression assays,

b io-informatics and Microbiology

• Experience in managing a research lab and supervising students, technicians,

and assistants; management of personnel, materials and equipment in

l aboratories

• Demonstrated ability to conduct independent research and publish results

• Excellent analytical skills

• H ighly creative and productive, competent and confident

• Strong, responsible, and reliable

• Largely self-directed, confident, able to work both independently and as a team-

member

• In tellectual ability to identify and analyze the problem, and technical and

scientific knowledge to solve all manners of questions, puzzles, and problems as

t hey arise

• Excellent computer skills combine with statistical skills

COMP U TER SK I L LS

Efficient in working with MS Outlook, Graph-pod Prism, Snagit 10, Symyx Notebook, End note

5, Adobe Photoshop, Notebook, ImageScope v11, Microsoft Excel, Microsoft Word, Microsoft

Power Point, Microsoft Access and Microsoft FrontPage and Auto Cad. Comfortable to work in

PC environments

References: Available on Request

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