Kenneth Ryan, Ph.D.
Curriculum Vitae
Bohemia, NY 11716
*********@*****.***
267-***-**** (cell)
OBJECTIVE
To obtain a scientist position with a Pharmaceutical/Consumer Products
company that would utilize my life sciences Ph.D. Excellent written and
oral communication skills. Proven ability to write the following types of
documents: grant proposals, scientific manuscripts, protocols,
abstracts/posters, scientific reviews.
SUMMARY OF QUALIFICATIONS
Advanced life sciences degree: Ph.D. in Biochemistry, Cellular, and
Molecular Biology.
. Academic research: Over sixteen years of post-doctoral and faculty
level research experience at the University of Cambridge and The
Children's Hospital of Philadelphia.
. Published author: Twenty peer-reviewed publications with four as
first author; and two review articles, one as first author and one as
co-first author.
. Grant writer: Six years of grant writing experience resulting in four
successfully funded grant proposals.
. Scientific communications/writing: Eighteen abstract/poster
presentations with thirteen as first-author and one as senior author.
Invited conference speaker. Prepared two IACUC protocols for
institutional approval and standard operating procedures; PowerPoint
slide presentations, progress and final reports for granting agencies;
laboratory protocols for students, technicians, and post-doctoral
fellows.
. Computer skills: C, Java, Perl language computer programming skills;
excellent Microsoft Office software skills (Word, PowerPoint, Excel).
Proficient in Adobe Photoshop, Illustrator, Acrobat; MacVector;
EndNote; AppleWorks; FileMaker Pro; ImageJ; Quantity One.
EDUCATION
Aug. 1985 to Nov. 1992: Ph.D. in Biochemistry, Cellular, and Molecular
Biology
The Johns Hopkins University School of Medicine (Department of
Biological Chemistry)
Jun. 1984 to Jan. 1985: Non-matriculated (Calculus III, Physical
Chemistry)
New York University
Aug. 1978 to May 1982: Bachelor of Science in Biology
SUNY at Stony Brook
Major: Biology Minor: French (emphasis on literature)
PROFESSIONAL EXPERIENCE
Dec. 2010 to Jul. 2011 Scientific Review Officer (Contractor): Kelly
Government Services, the Center for Scientific Review
(CSR), National Institutes of Health (NIH)
Writing, communication and administrative responsibilities:
. Draw upon my knowledge as a scientist to perform
administrative review and scientific focus evaluation of
Fellowship Study Sections F05, F09, and F14.
. Communicate with and advise Principal Investigators
(PIs) regarding their applications and conflicts.
. Identify and recruit qualified reviewers following NIH
Best Practices to ensure geographical, gender and ethnic
diversity, as well as proper scientific expertise.
. Select Chairs for the Study Section meetings.
. Educate and brief the review panel on Government
Procedures and Best Practices as a basis for reviewing
and evaluating grant applications and contract
proposals.
. Supervise Extramural Scientific Administrators (ESAs)
who are responsible for the administrative aspects of
the meetings.
. Assign reviewers to applications based on expertise.
. Manage conflicts of interest according to NIH policy.
. Help the Scientific Review Officer (SRO) to run the
review meetings and ensure fair and expert review for
each application.
. Edit the written critiques prepared by expert reviewers
and prepare Summary Statements for review committee
findings and recommendations, a report of the panel's
discussion submitted to the Institute Councils and the
PIs, for use by an NIH Institute or Center advisory
committee.
. Help the SRO communicate with Program Officers regarding
applications, reviewers, and in case of appeal.
Jul. 2001 to Jul. 2009: Research Assistant Professor of Pediatrics:
The Children's Hospital of Philadelphia and The
University of Pennsylvania School of Medicine
Writing and communication responsibilities:
. Published five research articles in peer-reviewed
journals.
. Grant writer for original laboratory research.
Three of four grant submissions successfully
funded.
. Grant project manager:
1) Oversee the progress and administration of two
NIH grants, one R01 (as Principal Investigator
[PI]) and a Core facility (as Core Director and
a Co-Investigator) for a P01.
2) Write, assemble, and submit all materials for
grant applications, progress reports, and all
other required documentation for the granting
agencies.
3) Write molecular and developmental biology
protocols for laboratory and Core facility use.
Other responsibilities:
. Direct, lead, and perform grant-related research.
Jan. 2000 to Jun. 2001: Post-doctoral Research Scientist (faculty
track): The Children's Hospital of Philadelphia
Writing and communication responsibilities:
. Published three research articles in peer-reviewed
journals.
. Grant writer for original research project. One
of two grant submissions successfully funded.
Other responsibilities:
. Direct, lead, and perform grant-related research.
Mar. 1993 to Dec. 1999: Post-doctoral Research Scientist: University
of Cambridge, UK
Writing and communication responsibilities:
. Published eight research articles in peer-reviewed
journals and one review article.
. Presented research results at one institute-wide
seminar, three annual institute retreats, and one
international scientific meeting (poster, Seventh
International Xenopus meeting, Sardinia, Italy).
Other responsibilities:
. Performed laboratory research in Xenopus
developmental biology.
. Trained undergraduate and graduate students in
molecular and developmental biology techniques.
. Design and implementation of molecular and
developmental biology experiments.
AWARDS AND HONORS
1980 Phi Sigma Iota, National Foreign Language Honor Society, SUNY at
Stony Brook
2001 Florence R.C. Murray Fellowship Award
2002 American Heart Association (National) Scientist Development Grant
(Ryan PI, 0235505N)
2002 Gordon Research Conference on Cardiac Regulatory Mechanisms
2002 NIH/NHLBI R01 award (Ryan PI, R01 HL070168)
2003 Cold Spring Harbor Molecular Embryology of the Mouse Course
2003 NIH/NHLBI SCCOR award (Levy PI, P50 HL074731) as a Co-
Investigator and Core Director
PUBLICATIONS
1. Wang, F., and Ryan, K. Eomesodermin is required for trafficking of
Smad2 between adjacent embryonic cells. In revision.
2. Picozzi, P., Wang, F., Cronk, K., and Ryan, K. (2009). Eomesodermin
requires TGF-b/activin signaling and binds Smad2 to activate
mesodermal genes. Jour. Biol. Chem. 284: 2397-2408.
3. Intlekofer, A.M., Takamoto, M., Wherry, E.J., Longworth, S.A.,
Northrup, J.T., Palanivel, V.R., Mullen, A.C., Gasink, C.R., Kaech,
S.M., Miller, J.D., Gapin, L., Ryan, K., Russ, A.P., Lindsten, T.,
Orange, J.S., Goldrath, A.W., Ahmed, R., and Reiner, S.L. (2005).
Programming of effector memory and CD8+ T cell fate coupled by the
transcription factors T-bet and Eomesodermin. Nature Immunol. 6:1236-
1244.
4. Perlstein, I., Burton, D.Y., Ryan, K., DeFelice, S., Simmers, E.,
Campbel, B., Connolly, J.M., Hoffman, A., and Levy, R.J. (2005).
Posttranslational control of a cardiac ion channel transgene in vivo:
clarithromycin-hMiRP1-Q9E interactions. Human Gene Ther. 16:906-910.
5. McConnell, J., Petrie, L., Stennard, F., Ryan, K. and Nichols, J.
(2005). Eomesodermin is expressed in mouse oocytes and pre-
implantation embryos. Mol. Reprod. Dev. 71:399-404.
6. Ryan, K., Russ, A.P., Levy, R.J., Wehr, D.J., You, J., and Easterday,
M.C. (2004). Modulation of Eomes activity alters the size of the
developing heart: Implications for in utero cardiac gene therapy.
Human Gene Ther. 15:842-855.
7. Levy, R.J., Song, C., Tallapragada, S., DeFelice, S., Hinson, J.T.,
Vyavahare, N., Connolly, J., Ryan, K., and Li, Q. (2001). Localized
adenovirus gene delivery using antiviral IgG complexation. Gene Ther.
8:659-667.
8. Taelman, V., Avalosse, B., Opdecamp, K., Ryan, K, and Bellefroid, E.J.
(2001). Cloning and expression of the bHLH encoding gene, Xath2,
expressed in the forebrain of Xenopus embryos. Mech. Dev. 101:199-
202.
9. Ryan, K., Garrett, N., Bourillot, P.-Y., Stennard, F., and Gurdon, J.B.
(2000). The Xenopus Eomesodermin promoter and its concentration-
dependent response to activin. Mech. Dev. 94:133-146.
10. Russ, A. P., Wattler, S., Colledge, W. H., Aparicio, S. A. J. R.,
Carlton, M. B. L., Pearce, J. J., Barton, S. C., Surani, M. A., Ryan,
K., Nehls, M. C., Wilson, V., and Evans M. J. (2000). Eomesodermin is
required for mouse trophoblast development and mesoderm formation.
Nature 404:95-99.
11. Gurdon, J. B., Standley, H., Dyson, S., Butler, K., Langon, T., Ryan,
K., Stennard, F., Shimizu, K., and Zorn, A. (1999). Single cells can
sense their position in a morphogen gradient. Development 126:5309-
5317.
12. Stennard, F., Zorn, A. M., Ryan, K., Garrett, N., and Gurdon, J. B.
(1999). Differential expression of VegT and Antipodean protein
isoforms in Xenopus. Mech. Dev. 86:87-98.
13. Ryan, K., Butler, K., Bellefroid, E. and Gurdon, J. B. (1998).
Eomesodermin is expressed in neural differentiation. Mech. Dev. 75:155-
158.
14. Gurdon, J. B., Ryan, K., Stennard, F., McDowell, N., Zorn, A. M.,
Crease, D. J., and Dyson, S. (1997). Cell response to different
concentrations of a morphogen: activin effects on Xenopus animal caps.
CSH Symposia Quant. Biol. 62:151-157.
15. Ryan, K., Garrett, N., Mitchell, A., and Gurdon, J. B. (1996).
Eomesodermin, a key early gene in Xenopus mesoderm differentiation.
Cell 87:989-1000.
16. Zernicka-Goetz, M., Pines, J., Ryan, K., Siemering, K. R., Haseloff,
J., Evans, M. J., and Gurdon, J. B. (1996). An indelible lineage
marker for Xenopus using a mutated green fluorescent protein.
Development 122:3719-3724.
17. Gurdon, J. B., Mitchell, A., and Ryan, K. (1996). An experimental
system for analyzing response to a morphogen gradient. Proc. Natl.
Acad. Sci. 93:9334-9338.
18. Gurdon, J. B., Ryan, K., Stennard, F., McDowell, N., Crease, D.,
Dyson, S., Zorn, A., Garrett, N., Mitchell, A., Carnac, G. (1996).
Long range signalling process in embryonic development. Int. J. Dev.
Biol. Suppl. 1:57S-58S.
19. Brun, R. P., Ryan, K., and Sollner-Webb, B. (1994). Factor C*, the
Specific Initiation Component of Mouse RNA Polymerase I Holoenzyme, Is
Inactivated Early in the Transcription Process. Mol. Cell. Biol.
14:5010-5021.
20. Sollner-Webb, B., Pape, L., Ryan, K., Mougey, E. B., Porretta, R.,
Nikolov, E., Paalman, M. H., Lazdins, I., and Martin, C. (1991).
Expression of Mouse and Frog rRNA Genes: Transcription and Processing.
Mol. Cell. Biochem. 104:149-154.
21. Pikaard, C. S., Pape, L. K., Henderson, S. L., Ryan, K., Paalman, M.
H., Lopata, M. A., Reeder, R. H., and Sollner-Webb, B. (1990).
Enhancers for Polymerase I in Mouse Ribosomal DNA. Mol. Cell. Biol.
10:4816-4825.
22. Henderson, S. L., Ryan, K., and Sollner-Webb, B. (1989). The Promoter-
Proximal rDNA Terminator Augments Initiation by Preventing Disruption
of the Stable Transcription Complex Caused by Polymerase Read-In.
Genes Dev. 3:212-223.
REVIEW ARTICLES
1. Ryan, K. and Chin, A.J. (2003). T-box genes and cardiac development.
Birth Defects Res. (Part C) 69:25-37.
2. Stennard[1], F., Ryan1, K., and Gurdon, J.B. (1997). Markers of
vertebrate mesoderm induction. Curr. Opin. Genet. Dev. 7:620-627.
INVITED PLENARY SPEAKER
1. Ryan, K., Hazelwood, S.K., Levy, R.J. (2001). Eomesodermin is a crucial
T-box gene in vertebrate cardiac development. American Heart
Association Scientific Sessions 2001, Anaheim, CA, Nov. 11-14.
ABSTRACTS AND POSTER PRESENTATIONS
1. Picozzi, P., Messick, C., Cronk, K., Luo, X., Liu, W., You, J. Ryan, K.
Eomesodermin cooperates with Smad2 to activate a subset of mesodermal
target genes in Xenopus embryos. Meeting Abstract Book, p. 113,
Abstract #179. (Poster presentation, the Fourteenth Annual Weinstein
Cardiovascular Development Conference, Indiana University,
Indianapolis, IN, May 10-12, 2007.)
2. Connolly, J.M., Oyama, M.A., Gorman, R.C., Gorman III, J.H., Fulmer,
J.T., Ryan, K., Levy, R.J. Serotonin transporter blockade with
dexfenfluoramine or fluoxetine increases serotonin-mediated ERT1/2
phosphorylation in heart valve interstitial cells: implications for
serotonin-related heart valve disease. Circulation Suppl. 114: II-300
(1551). (Poster presentation, American Heart Association Scientific
Sessions 2006 Meeting, Chicago, IL, October 31, 2006.)
3. Nappo, G., Santoriello, C., Ryan, K., and Mione, M. Zygotic functions
of eomesodermin. Meeting Absract Book, p. 451, Abstract #451. (Poster
presentation, 7th International Conference on Zebrafish Development
and Genetics, Madison, WI, June14-18, 2006.)
4. Paola Picozzi, Weibin Liu, Christina Messick, Kevin Cronk, Xuan Luo,
Jingtao You, and Kenneth Ryan. Eomesodermin cooperates with Smad2 to
activate a subset of mesodermal target genes in Xenopus embryos.
Meeting Abstract Book, p. 37. (Abstract publication, the Third Annual
Joseph Stokes Jr. Research Institute Scientific Symposium, American
College, Bryn Mawr, PA, April 21, 2006).
5. Ryan, K., Russ, A.P., Levy, R.J., Wehr, D.J., You, J., and Easterday,
M.C. Manipulation of the level of Eomes activity alters the size of
the developing heart: implications for cardiac gene therapy. Meeting
Abstract Book, p. 60, Abstract #B39. (Poster presentation, the Twelfth
Annual Weinstein Cardiovascular Development Conference, The University
of Arizona, Tucson, AZ, May 19-22, 2005).
6. Ryan, K., Russ, A.P., Levy, R.J., Wehr, D.J., You, J., and Easterday,
M.C. Modulation of the level of Eomes activity alters the size of the
developing heart: Implications for cardiac gene therapy. Meeting
Abstract Book, p. 52. (Abstract publication, the Second Annual Joseph
Stokes Jr. Research Institute Scientific Symposium, American College,
Bryn Mawr, PA, April 29, 2005).
7. Ryan, K., Russ, A.P., Levy, R.J., Wehr, D.J., You, J., and Easterday,
M.C. Modulation of Eomes activity alters the size of the developing
heart: Implications for cardiac gene therapy. Meeting Abstract Book,
p. 106, Abstract #90. (Poster Presentation, Institute for Medicine and
Engineering 2004 Symposium, University of Pennsylvania, Philadelphia,
PA, December 2, 2004).
8. Ryan, K., Russ, A.P., Levy, R.J., Wehr, D.J., You, J., and Easterday,
M.C. Manipulation of the level of Eomes activity alters the size of
the developing heart: Implications for cardiac gene therapy. Meeting
Abstract Book, p. 107, Abstract #137. (Poster Presentation, Tenth
International Xenopus Meeting, Marine Biological Laboratory, Woods
Hole, MA, September 14-18, 2004).
9. Ryan, K., Wehr, D.J., Russ, A.P. and Levy, R.J. Evidence that
Eomesodermin Is Important in Cardiac Development. Meeting Abstract
Book, p. 65. (Seminar Presentation, The First Annual Symposium of the
Joseph Stokes Jr. Research Institute of the Children's Hospital of
Philadelphia, Bryn Mawr, PA, September 24, 2003).
10. Ryan, K. and Levy, R.J. Evidence that Eomesodermin Is Important in
Cardiac Development. (Poster presentation, Weinstein 2003
Cardiovascular Development Conference, Boston, MA, May 15-17, 2003.)
11. Ryan, K., Wehr, D.J., Jennings, J.S. and Levy, R.J. Eomesodermin is
Required in Development for Cardiac Mesoderm Induction, Looping, and
Outflow Tract Formation. Circulation Suppl. 106: II-285 (1438).
(Basic Science poster presentation at the American Heart Association
Scientific Sessions 2002 Meeting, Chicago, IL, November 20, 2002.)
12. Ryan, K., Wehr, D.J., and Levy, R.J. Eomesodermin is a Crucial T-box
Gene in Vertebrate Cardiac Development. (Poster presentation, Gordon
Research Conference on Cardiac Regulatory Mechanisms, New London, CT,
July 28 - August 2, 2002.)
13. Ryan, K., Wehr, D.J., Jennings, J.S. and Levy, R.J. Eomesodermin Is a
Crucial T-Box Gene in Vertebrate Cardiac Development. (Poster
presentation, Weinstein 2002 Cardiovascular Development Conference,
Salt Lake City, Utah, May 16-18, 2002.)
14. Ryan, K., Jennings, J., Wehr, D., and Levy, R.J. Eomesodermin is a
crucial gene in heart development. Poster No. 30, Abstract Book, p.
39. (Institute for Medicine and Engineering 2001 Symposium, University
of Pennsylvania School of Medicine, Philadelphia, PA, December 4,
2001.)
15. Ryan, K., Hazelwood, S.K., and Levy, R.J. Eomesodermin (Eomes) is
expressed in heart development and disease. (Presented at the Annual
Poster Day Meeting, Children's Hospital of Philadelphia, February
2001.)
16. Song, C., Klugherz, B., Defelice, S., Ryan, K., Wilensky, R.L., and
Levy, R.J. Antibody tethered adenovirus for stent-based vector
delivery in pig coronaries. (Presented at the American Heart
Association Annual Meeting, New Orleans, November, 2000.)
17. Ryan, K., Hazelwood, S.K., and Levy, R.J. Eomesodermin (Eomes) in
heart development and disease. (Presented at the Pennsylvania Muscle
Institute Eighth Annual Retreat and Symposium, Development of the
Cardiovascular System, Philadelphia, October 20, 2000.)
18. Ryan, K., Garrett, N., Bourillot, P.-Y., Stennard, F., and Gurdon,
J.B. The Xenopus Eomesodermin promoter and its concentration-dependent
response to activin. Meeting Abstract Book, p. 93. (Poster
presentation, Eighth International Xenopus Conference, Estes Park, CO,
August 16-20, 2000.)
SPECIAL SKILLS
Molecular and developmental biology; excellent oral and verbal
communication in French.
[1]Equal first-author contribution.