Kenneth Ryan, Ph.D.

Curriculum Vitae

** *********** *****

Bohemia, NY 11716

abg0eq@r.postjobfree.com

267-***-**** (cell)

OBJECTIVE

To obtain a scientist position with a Pharmaceutical/Consumer Products

company that would utilize my life sciences Ph.D. Excellent written and

oral communication skills. Proven ability to write the following types of

documents: grant proposals, scientific manuscripts, protocols,

abstracts/posters, scientific reviews.

SUMMARY OF QUALIFICATIONS

Advanced life sciences degree: Ph.D. in Biochemistry, Cellular, and

Molecular Biology.

. Academic research: Over sixteen years of post-doctoral and faculty

level research experience at the University of Cambridge and The

Children's Hospital of Philadelphia.

. Published author: Twenty peer-reviewed publications with four as

first author; and two review articles, one as first author and one as

co-first author.

. Grant writer: Six years of grant writing experience resulting in four

successfully funded grant proposals.

. Scientific communications/writing: Eighteen abstract/poster

presentations with thirteen as first-author and one as senior author.

Invited conference speaker. Prepared two IACUC protocols for

institutional approval and standard operating procedures; PowerPoint

slide presentations, progress and final reports for granting agencies;

laboratory protocols for students, technicians, and post-doctoral

fellows.

. Computer skills: C, Java, Perl language computer programming skills;

excellent Microsoft Office software skills (Word, PowerPoint, Excel).

Proficient in Adobe Photoshop, Illustrator, Acrobat; MacVector;

EndNote; AppleWorks; FileMaker Pro; ImageJ; Quantity One.

EDUCATION

Aug. 1985 to Nov. 1992: Ph.D. in Biochemistry, Cellular, and Molecular

Biology

The Johns Hopkins University School of Medicine (Department of

Biological Chemistry)

Jun. 1984 to Jan. 1985: Non-matriculated (Calculus III, Physical

Chemistry)

New York University

Aug. 1978 to May 1982: Bachelor of Science in Biology

SUNY at Stony Brook

Major: Biology Minor: French (emphasis on literature)

PROFESSIONAL EXPERIENCE

Dec. 2010 to Jul. 2011 Scientific Review Officer (Contractor): Kelly

Government Services, the Center for Scientific Review

(CSR), National Institutes of Health (NIH)

Writing, communication and administrative responsibilities:

. Draw upon my knowledge as a scientist to perform

administrative review and scientific focus evaluation of

Fellowship Study Sections F05, F09, and F14.

. Communicate with and advise Principal Investigators

(PIs) regarding their applications and conflicts.

. Identify and recruit qualified reviewers following NIH

Best Practices to ensure geographical, gender and ethnic

diversity, as well as proper scientific expertise.

. Select Chairs for the Study Section meetings.

. Educate and brief the review panel on Government

Procedures and Best Practices as a basis for reviewing

and evaluating grant applications and contract

proposals.

. Supervise Extramural Scientific Administrators (ESAs)

who are responsible for the administrative aspects of

the meetings.

. Assign reviewers to applications based on expertise.

. Manage conflicts of interest according to NIH policy.

. Help the Scientific Review Officer (SRO) to run the

review meetings and ensure fair and expert review for

each application.

. Edit the written critiques prepared by expert reviewers

and prepare Summary Statements for review committee

findings and recommendations, a report of the panel's

discussion submitted to the Institute Councils and the

PIs, for use by an NIH Institute or Center advisory

committee.

. Help the SRO communicate with Program Officers regarding

applications, reviewers, and in case of appeal.

Jul. 2001 to Jul. 2009: Research Assistant Professor of Pediatrics:

The Children's Hospital of Philadelphia and The

University of Pennsylvania School of Medicine

Writing and communication responsibilities:

. Published five research articles in peer-reviewed

journals.

. Grant writer for original laboratory research.

Three of four grant submissions successfully

funded.

. Grant project manager:

1) Oversee the progress and administration of two

NIH grants, one R01 (as Principal Investigator

[PI]) and a Core facility (as Core Director and

a Co-Investigator) for a P01.

2) Write, assemble, and submit all materials for

grant applications, progress reports, and all

other required documentation for the granting

agencies.

3) Write molecular and developmental biology

protocols for laboratory and Core facility use.

Other responsibilities:

. Direct, lead, and perform grant-related research.

Jan. 2000 to Jun. 2001: Post-doctoral Research Scientist (faculty

track): The Children's Hospital of Philadelphia

Writing and communication responsibilities:

. Published three research articles in peer-reviewed

journals.

. Grant writer for original research project. One

of two grant submissions successfully funded.

Other responsibilities:

. Direct, lead, and perform grant-related research.

Mar. 1993 to Dec. 1999: Post-doctoral Research Scientist: University

of Cambridge, UK

Writing and communication responsibilities:

. Published eight research articles in peer-reviewed

journals and one review article.

. Presented research results at one institute-wide

seminar, three annual institute retreats, and one

international scientific meeting (poster, Seventh

International Xenopus meeting, Sardinia, Italy).

Other responsibilities:

. Performed laboratory research in Xenopus

developmental biology.

. Trained undergraduate and graduate students in

molecular and developmental biology techniques.

. Design and implementation of molecular and

developmental biology experiments.

AWARDS AND HONORS

1980 Phi Sigma Iota, National Foreign Language Honor Society, SUNY at

Stony Brook

2001 Florence R.C. Murray Fellowship Award

2002 American Heart Association (National) Scientist Development Grant

(Ryan PI, 0235505N)

2002 Gordon Research Conference on Cardiac Regulatory Mechanisms

2002 NIH/NHLBI R01 award (Ryan PI, R01 HL070168)

2003 Cold Spring Harbor Molecular Embryology of the Mouse Course

2003 NIH/NHLBI SCCOR award (Levy PI, P50 HL074731) as a Co-

Investigator and Core Director

PUBLICATIONS

1. Wang, F., and Ryan, K. Eomesodermin is required for trafficking of

Smad2 between adjacent embryonic cells. In revision.

2. Picozzi, P., Wang, F., Cronk, K., and Ryan, K. (2009). Eomesodermin

requires TGF-b/activin signaling and binds Smad2 to activate

mesodermal genes. Jour. Biol. Chem. 284: 2397-2408.

3. Intlekofer, A.M., Takamoto, M., Wherry, E.J., Longworth, S.A.,

Northrup, J.T., Palanivel, V.R., Mullen, A.C., Gasink, C.R., Kaech,

S.M., Miller, J.D., Gapin, L., Ryan, K., Russ, A.P., Lindsten, T.,

Orange, J.S., Goldrath, A.W., Ahmed, R., and Reiner, S.L. (2005).

Programming of effector memory and CD8+ T cell fate coupled by the

transcription factors T-bet and Eomesodermin. Nature Immunol. 6:1236-

1244.

4. Perlstein, I., Burton, D.Y., Ryan, K., DeFelice, S., Simmers, E.,

Campbel, B., Connolly, J.M., Hoffman, A., and Levy, R.J. (2005).

Posttranslational control of a cardiac ion channel transgene in vivo:

clarithromycin-hMiRP1-Q9E interactions. Human Gene Ther. 16:906-910.

5. McConnell, J., Petrie, L., Stennard, F., Ryan, K. and Nichols, J.

(2005). Eomesodermin is expressed in mouse oocytes and pre-

implantation embryos. Mol. Reprod. Dev. 71:399-404.

6. Ryan, K., Russ, A.P., Levy, R.J., Wehr, D.J., You, J., and Easterday,

M.C. (2004). Modulation of Eomes activity alters the size of the

developing heart: Implications for in utero cardiac gene therapy.

Human Gene Ther. 15:842-855.

7. Levy, R.J., Song, C., Tallapragada, S., DeFelice, S., Hinson, J.T.,

Vyavahare, N., Connolly, J., Ryan, K., and Li, Q. (2001). Localized

adenovirus gene delivery using antiviral IgG complexation. Gene Ther.

8:659-667.

8. Taelman, V., Avalosse, B., Opdecamp, K., Ryan, K, and Bellefroid, E.J.

(2001). Cloning and expression of the bHLH encoding gene, Xath2,

expressed in the forebrain of Xenopus embryos. Mech. Dev. 101:199-

202.

9. Ryan, K., Garrett, N., Bourillot, P.-Y., Stennard, F., and Gurdon, J.B.

(2000). The Xenopus Eomesodermin promoter and its concentration-

dependent response to activin. Mech. Dev. 94:133-146.

10. Russ, A. P., Wattler, S., Colledge, W. H., Aparicio, S. A. J. R.,

Carlton, M. B. L., Pearce, J. J., Barton, S. C., Surani, M. A., Ryan,

K., Nehls, M. C., Wilson, V., and Evans M. J. (2000). Eomesodermin is

required for mouse trophoblast development and mesoderm formation.

Nature 404:95-99.

11. Gurdon, J. B., Standley, H., Dyson, S., Butler, K., Langon, T., Ryan,

K., Stennard, F., Shimizu, K., and Zorn, A. (1999). Single cells can

sense their position in a morphogen gradient. Development 126:5309-

5317.

12. Stennard, F., Zorn, A. M., Ryan, K., Garrett, N., and Gurdon, J. B.

(1999). Differential expression of VegT and Antipodean protein

isoforms in Xenopus. Mech. Dev. 86:87-98.

13. Ryan, K., Butler, K., Bellefroid, E. and Gurdon, J. B. (1998).

Eomesodermin is expressed in neural differentiation. Mech. Dev. 75:155-

158.

14. Gurdon, J. B., Ryan, K., Stennard, F., McDowell, N., Zorn, A. M.,

Crease, D. J., and Dyson, S. (1997). Cell response to different

concentrations of a morphogen: activin effects on Xenopus animal caps.

CSH Symposia Quant. Biol. 62:151-157.

15. Ryan, K., Garrett, N., Mitchell, A., and Gurdon, J. B. (1996).

Eomesodermin, a key early gene in Xenopus mesoderm differentiation.

Cell 87:989-1000.

16. Zernicka-Goetz, M., Pines, J., Ryan, K., Siemering, K. R., Haseloff,

J., Evans, M. J., and Gurdon, J. B. (1996). An indelible lineage

marker for Xenopus using a mutated green fluorescent protein.

Development 122:3719-3724.

17. Gurdon, J. B., Mitchell, A., and Ryan, K. (1996). An experimental

system for analyzing response to a morphogen gradient. Proc. Natl.

Acad. Sci. 93:9334-9338.

18. Gurdon, J. B., Ryan, K., Stennard, F., McDowell, N., Crease, D.,

Dyson, S., Zorn, A., Garrett, N., Mitchell, A., Carnac, G. (1996).

Long range signalling process in embryonic development. Int. J. Dev.

Biol. Suppl. 1:57S-58S.

19. Brun, R. P., Ryan, K., and Sollner-Webb, B. (1994). Factor C*, the

Specific Initiation Component of Mouse RNA Polymerase I Holoenzyme, Is

Inactivated Early in the Transcription Process. Mol. Cell. Biol.

14:5010-5021.

20. Sollner-Webb, B., Pape, L., Ryan, K., Mougey, E. B., Porretta, R.,

Nikolov, E., Paalman, M. H., Lazdins, I., and Martin, C. (1991).

Expression of Mouse and Frog rRNA Genes: Transcription and Processing.

Mol. Cell. Biochem. 104:149-154.

21. Pikaard, C. S., Pape, L. K., Henderson, S. L., Ryan, K., Paalman, M.

H., Lopata, M. A., Reeder, R. H., and Sollner-Webb, B. (1990).

Enhancers for Polymerase I in Mouse Ribosomal DNA. Mol. Cell. Biol.

10:4816-4825.

22. Henderson, S. L., Ryan, K., and Sollner-Webb, B. (1989). The Promoter-

Proximal rDNA Terminator Augments Initiation by Preventing Disruption

of the Stable Transcription Complex Caused by Polymerase Read-In.

Genes Dev. 3:212-223.

REVIEW ARTICLES

1. Ryan, K. and Chin, A.J. (2003). T-box genes and cardiac development.

Birth Defects Res. (Part C) 69:25-37.

2. Stennard[1], F., Ryan1, K., and Gurdon, J.B. (1997). Markers of

vertebrate mesoderm induction. Curr. Opin. Genet. Dev. 7:620-627.

INVITED PLENARY SPEAKER

1. Ryan, K., Hazelwood, S.K., Levy, R.J. (2001). Eomesodermin is a crucial

T-box gene in vertebrate cardiac development. American Heart

Association Scientific Sessions 2001, Anaheim, CA, Nov. 11-14.

ABSTRACTS AND POSTER PRESENTATIONS

1. Picozzi, P., Messick, C., Cronk, K., Luo, X., Liu, W., You, J. Ryan, K.

Eomesodermin cooperates with Smad2 to activate a subset of mesodermal

target genes in Xenopus embryos. Meeting Abstract Book, p. 113,

Abstract #179. (Poster presentation, the Fourteenth Annual Weinstein

Cardiovascular Development Conference, Indiana University,

Indianapolis, IN, May 10-12, 2007.)

2. Connolly, J.M., Oyama, M.A., Gorman, R.C., Gorman III, J.H., Fulmer,

J.T., Ryan, K., Levy, R.J. Serotonin transporter blockade with

dexfenfluoramine or fluoxetine increases serotonin-mediated ERT1/2

phosphorylation in heart valve interstitial cells: implications for

serotonin-related heart valve disease. Circulation Suppl. 114: II-300

(1551). (Poster presentation, American Heart Association Scientific

Sessions 2006 Meeting, Chicago, IL, October 31, 2006.)

3. Nappo, G., Santoriello, C., Ryan, K., and Mione, M. Zygotic functions

of eomesodermin. Meeting Absract Book, p. 451, Abstract #451. (Poster

presentation, 7th International Conference on Zebrafish Development

and Genetics, Madison, WI, June14-18, 2006.)

4. Paola Picozzi, Weibin Liu, Christina Messick, Kevin Cronk, Xuan Luo,

Jingtao You, and Kenneth Ryan. Eomesodermin cooperates with Smad2 to

activate a subset of mesodermal target genes in Xenopus embryos.

Meeting Abstract Book, p. 37. (Abstract publication, the Third Annual

Joseph Stokes Jr. Research Institute Scientific Symposium, American

College, Bryn Mawr, PA, April 21, 2006).

5. Ryan, K., Russ, A.P., Levy, R.J., Wehr, D.J., You, J., and Easterday,

M.C. Manipulation of the level of Eomes activity alters the size of

the developing heart: implications for cardiac gene therapy. Meeting

Abstract Book, p. 60, Abstract #B39. (Poster presentation, the Twelfth

Annual Weinstein Cardiovascular Development Conference, The University

of Arizona, Tucson, AZ, May 19-22, 2005).

6. Ryan, K., Russ, A.P., Levy, R.J., Wehr, D.J., You, J., and Easterday,

M.C. Modulation of the level of Eomes activity alters the size of the

developing heart: Implications for cardiac gene therapy. Meeting

Abstract Book, p. 52. (Abstract publication, the Second Annual Joseph

Stokes Jr. Research Institute Scientific Symposium, American College,

Bryn Mawr, PA, April 29, 2005).

7. Ryan, K., Russ, A.P., Levy, R.J., Wehr, D.J., You, J., and Easterday,

M.C. Modulation of Eomes activity alters the size of the developing

heart: Implications for cardiac gene therapy. Meeting Abstract Book,

p. 106, Abstract #90. (Poster Presentation, Institute for Medicine and

Engineering 2004 Symposium, University of Pennsylvania, Philadelphia,

PA, December 2, 2004).

8. Ryan, K., Russ, A.P., Levy, R.J., Wehr, D.J., You, J., and Easterday,

M.C. Manipulation of the level of Eomes activity alters the size of

the developing heart: Implications for cardiac gene therapy. Meeting

Abstract Book, p. 107, Abstract #137. (Poster Presentation, Tenth

International Xenopus Meeting, Marine Biological Laboratory, Woods

Hole, MA, September 14-18, 2004).

9. Ryan, K., Wehr, D.J., Russ, A.P. and Levy, R.J. Evidence that

Eomesodermin Is Important in Cardiac Development. Meeting Abstract

Book, p. 65. (Seminar Presentation, The First Annual Symposium of the

Joseph Stokes Jr. Research Institute of the Children's Hospital of

Philadelphia, Bryn Mawr, PA, September 24, 2003).

10. Ryan, K. and Levy, R.J. Evidence that Eomesodermin Is Important in

Cardiac Development. (Poster presentation, Weinstein 2003

Cardiovascular Development Conference, Boston, MA, May 15-17, 2003.)

11. Ryan, K., Wehr, D.J., Jennings, J.S. and Levy, R.J. Eomesodermin is

Required in Development for Cardiac Mesoderm Induction, Looping, and

Outflow Tract Formation. Circulation Suppl. 106: II-285 (1438).

(Basic Science poster presentation at the American Heart Association

Scientific Sessions 2002 Meeting, Chicago, IL, November 20, 2002.)

12. Ryan, K., Wehr, D.J., and Levy, R.J. Eomesodermin is a Crucial T-box

Gene in Vertebrate Cardiac Development. (Poster presentation, Gordon

Research Conference on Cardiac Regulatory Mechanisms, New London, CT,

July 28 - August 2, 2002.)

13. Ryan, K., Wehr, D.J., Jennings, J.S. and Levy, R.J. Eomesodermin Is a

Crucial T-Box Gene in Vertebrate Cardiac Development. (Poster

presentation, Weinstein 2002 Cardiovascular Development Conference,

Salt Lake City, Utah, May 16-18, 2002.)

14. Ryan, K., Jennings, J., Wehr, D., and Levy, R.J. Eomesodermin is a

crucial gene in heart development. Poster No. 30, Abstract Book, p.

39. (Institute for Medicine and Engineering 2001 Symposium, University

of Pennsylvania School of Medicine, Philadelphia, PA, December 4,

2001.)

15. Ryan, K., Hazelwood, S.K., and Levy, R.J. Eomesodermin (Eomes) is

expressed in heart development and disease. (Presented at the Annual

Poster Day Meeting, Children's Hospital of Philadelphia, February

2001.)

16. Song, C., Klugherz, B., Defelice, S., Ryan, K., Wilensky, R.L., and

Levy, R.J. Antibody tethered adenovirus for stent-based vector

delivery in pig coronaries. (Presented at the American Heart

Association Annual Meeting, New Orleans, November, 2000.)

17. Ryan, K., Hazelwood, S.K., and Levy, R.J. Eomesodermin (Eomes) in

heart development and disease. (Presented at the Pennsylvania Muscle

Institute Eighth Annual Retreat and Symposium, Development of the

Cardiovascular System, Philadelphia, October 20, 2000.)

18. Ryan, K., Garrett, N., Bourillot, P.-Y., Stennard, F., and Gurdon,

J.B. The Xenopus Eomesodermin promoter and its concentration-dependent

response to activin. Meeting Abstract Book, p. 93. (Poster

presentation, Eighth International Xenopus Conference, Estes Park, CO,

August 16-20, 2000.)

SPECIAL SKILLS

Molecular and developmental biology; excellent oral and verbal

communication in French.

[1]Equal first-author contribution.

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