Assistant University

Srihariraju Mulagapati

Doctoral student at University of Massachusetts

***********.*@*****.***

Summary

Analytical biochemist with experience in characterizing recombinant proteins (from various sources) for purity,

identity, integrity and function during biological drug development using a variety of biochemical and

biophysical techniques.

Experience

Teaching Assistant at University of Massachusetts

September 2011 - Present (2 years 1 month)

Instructed forensic chemistry 1 lecture, freshmen chemistry laboratory, and physical chemistry laboratory 2.

Supervised and coordinated students during experiments, examination review sessions, weekly office hours

and assisted course instructor with grading, proctoring and laboratory demonstrations. Lectured >200

students

Massachusetts Biomanufacturing Center (MBMC)

Trained new graduate, undergraduate and summer intern research students in protein chemistry techniques

Research Assistant at Massachusetts Biomanufacturing Center (UMass Lowell)

August 2010 - Present (3 years 2 months)

• Characterization of A. awamori-derived recombinant human lactoferrin (rhLF) and its structural variants.

(Ph.D. Thesis Research)

• Identification of multiple sources of micro-heterogeneities generated during mAbs production.

• Analysis of N-terminal integrity and purity of a recombinant IL-1 receptor antagonist expressed in E.coli.

• Characterization of the amino acid sequence and N-linked glycosylation of a snake venom glycoprotein

(thrombin-like serine protease).

• Assisted in developing new analytical methods for MBMC biopharmaceutical collaborators.

Research Assistant at Analytical & Environmental Chemistry Lab(UMass Lowell)

January 2010 - December 2010 (1 year)

Determination of amino acid content of Cd binding peptide in blue mussel blood plasma

Intern at Shantha Biotechnics Ltd

May 2008 - July 2008 (3 months)

Development of process specific ELISA for detecting host cell protein (HCP) in E.coli cells (Determined the

concentration of E. coli HCP during recombinant papillomavirus expression)

Intern at Biological E. Limited,

December 2007 - April 2008 (5 months)

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Production of poly-valent anti snake venom serum (Prepared product from hyperimmunised equines against

the venom. Product was subsequently enzyme refined, purified and concentrated)

Education

University of Massachusetts at Lowell

Doctor of Philosophy (PhD), Biochemistry, 2011 - 2013

University of Massachusetts at Lowell

Master's degree, Biotechnology, 2009 - 2011

Jawaharlal Nehru Technological University

Bachelor's degree, Biotechnology, 2004 - 2008

Skills & Expertise

LC-MS

HPLC

Light Scattering

Spectroscopy

Circular Dichroism

Surface Plasmon Resonance

Differential Scanning Calorimetry

SDS-PAGE

Western Blotting

Isoelectric Focusing

Gel Electrophoresis

PCR

Molecular Biology

UV/Vis

Biotechnology

Microbiology

Cell Culture

DNA extraction

Chromatography

Bioinformatics

ELISA

Biochemistry

Molecular Cloning

Protein Purification

Laboratory

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Publications

Reduction of product-related species during the fermentation and purification of a recombinant IL-1

receptor antagonist at the laboratory and pilot scale

Biotechnology journal August 2, 2013

Authors: Srihariraju Mulagapati

Honors and Awards

• Outstanding Graduate Student

UMass Lowell

April 2012

• Intern of the year award

Shanta Biotechnics Ltd

December 2008

• Merit scholarship

Jawaharlal Nehru Technological University

May 2005

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Srihariraju Mulagapati

Doctoral student at University of Massachusetts

***********.*@*****.***

Contact Srihariraju on LinkedIn

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