Quality Assurance Control

KIRAN K. PEDADA

****, **** **** ******, *********, OH 44114

Tel: 234-***-****, e-mail: *************@*****.***

PROFILE

. Self-motivated and highly energetic professional with 7+ years of

extensive experience in analytical and bioanalytical method development,

validation of small molecules using HPLC, LC-MS/MS techniques.

. Hands-on experience in help designing and preparation of clinical

pharmacokinetic protocols.

. Expertise in biological sample preparation and handling for DMPK studies.

. Proficient in handling AB Sciex 5500 QTrap, AB Sciex QStar, Bruker Ion-

Trap, Waters QQQ.

. Highly skilled in intact protein and peptide characterization using ion-

exchange, metal chelating and reversed phase LC-MS/MS techniques besides

gel-electrophoresis.

. Solid understanding of Drug Discovery & Drug Development concepts,

guideline including ICH, cGMP, GCP and FDA for Bioanalytical Method

Validation.

. Proficient in analytical software including: ChemStation, Analyst,

Xcalibur, Bruker Gold, Mascot, ChemDraw, Origin8, MATLAB besides Microsoft

office tools.

Core Strengths

Excellent trouble-shooting skills Teamwork skills

Strategic and analytical thinking Supervisory skills

Flexibility and adaptability Communication skill

EDUCATION

Doctorate in Clinical-Bioanalytical Chemistry 2007-

Present*

Cleveland State University, Cleveland, OH

Master in Pharmaceutical Analysis & Quality Assurance 2005-

2007

Roland Institute of Pharmaceutical Sciences, Berhampur, India

Bachelor of Pharmacy 2001-2005

Andhra University, Vishakhapatnam, India

RESEARCH EXPERIENCE

Cleveland State University, Cleveland, Ohio 2007-

Current

. Developed and validated analytical HPLC-MS methods for a neuroprotective

agent, novel thiazolidinedione mitoNEET ligand, NL-1 and NGP1-01 drug

molecules.

. Established full-length pharmacokinetic profile for neuroprotective

agents, NL-1 and NGP-01 in mouse serum, brain and retinal tissues using a

highly sensitive quantitative LC-MS/MS bioanalytical method in vivo.

. Developed LC-MS methods to analyze proteins and peptides using ion-

exchange HPLC- Tandem mass Spectrometry.

. Evaluated column characterization studies to identify the number of

binding sites using an ion-exchange HPLC.

. Mentored graduate students and supervised undergraduate students in

chemistry labs.

Roland Institute of Pharmaceutical Sciences, Berhampur, India 2005-

2007

. Developed and validated HPLC, UV- Spectrophtometric methods for various

pharmaceutical formulations consisting antifungal (voriconazole),

anticonvulsant (zonisamide) and antidepressant (bupropion hydrochloride)

agents.

. Performed analyses using analytical instrumentation including: HPLC,

LC/MS/MS, GC/MS, FTIR, UV-Visible spectrophotometer, TLC and KF titrator.

. Calibrated HPLC, UV-Visible spectrometer, Mass spectrometer, KF titrator,

pH meter and analytical balances.

Internship (Industrial Practical Training), NATCO Pharma Ltd., Hyderabad,

India

2005

. Received practical training on manufacturing, packing, operations of

solid as well as liquid dosage forms.

. Received training in Quality Control department, cGMP, GLP.

PUBLICATIONS

. Kiran K. Pedada, Xiang Zhou, H. Jogiraju, Richard T. Carroll, Werner J.

Geldenhuys, Li Lin, David J. Anderson, "Pharmacokinetic Studies of a

Novel Thiazolidinedione mitoNEET Ligand, NL-1, Using a Quantitative LC-

MS/MS Method in Dosed Mouse Serum and Brain" Proceedings of the 61st ASMS

Conference on Mass Spectrometry and Allied Topics (2013).

. Kiran K. Pedada, Xiang Zhou, H. Jogiraju, Richard T. Carroll, Werner J.

Geldenhuys, Li Lin, David J. Anderson, "A Rapid and Sensitive LC-MS

Method for the Quantification of a Neuroprotective Thiazolidinedione

Ligand, NL-1, in Mouse Serum" Proceedings of the 60th ASMS Conference on

Mass Spectrometry and Allied Topics (2012).

. Kiran K. Pedada, Xiang Zhou, H. Jogiraju, Richard T. Carroll, Werner J.

Geldenhuys, Li Lin, David J. Anderson, "A Rapid and Sensitive LC-MS

Method for the Quantification of a Neuroprotective Thiazolidinedione

Ligand, NL-1, in Mouse Serum" Proceedings of the 244th ACS Conference

(2012).

. A publication in a peer reviewed international journal, Srinubabu G, Raju

ChA, Sarath N, Kiran K. Pedada, Rao JV. Development and validation of a

HPLC method for the determination of voriconazole in pharmaceutical

formulation using an experimental design. Talanta.2007; 71:1424-29.

. Kiran K. Pedada, Xiang Zhou, H. Jogiraju, Richard T. Carroll, Werner J.

Geldenhuys, Li Lin, David J. Anderson. A Quantitative LC-MS method for a

Thiazolidinedione mitoNEET ligand suitable for pharmacokinetic analysis

(Submitted to Journal of Chromatography B).

MANUSCRIPTS UNDER PREPARATIONS

. Kiran K. Pedada, Xiang Zhou, H. Jogiraju, David J. Anderson. Reverse

Phase - Gradient Chromatofocusing - Mass Spectrometry: A New 2D LC- MS

Technology in protein and peptide analysis.

. Kiran K. Pedada, Xiang Zhou, H. Jogiraju, David J. Anderson. A novel

method for identifying the number of binding sites in an Ion exchange

HPLC column.

. Harini Jogiraju, Kiran K. Pedada, Xiang Zhou, Werner Geldenhuys, Cornelis

vander Schyf, S. Crish, David Anderson. Development of a highly sensitive

LC/MS/MS method for determination NGP1-01 in dosed mouse serum and

retinal tissue.

. Harini Jogiraju, Kiran K. Pedada, X. Zhou, D.J. Anderson. The effect of

the number of buffer components on separation of proteins in high-

performance gradient chromatofocusing ion-exchange using pH-gradients.

PRESENTATIONS

. Kiran K. Pedada, Xiang Zhou, H. Jogiraju, Richard T. Carroll, Werner J.

Geldenhuys, Li Lin, David J. Anderson, "Pharmacokinetic studies of a

novel thiazolidinedione mitoNEET ligand, Nl-1, using a quantitative LC-

MS/MS method in dosed mouse serum and brain". Poster presented at

"Research Day 2013", Cleveland State University.

. Kiran K. Peadada, David J.Anderson, et al. "Reverse Phase - Gradient

Chromatofocusing - Mass Spectrometry: A New 2D LC- MS Technology in

Protein and Peptide Analysis". Poster presented at "Interdisciplinary

Research Conference", Cleveland State University.

. Kiran K. Peadada, David J.Anderson, et al. "A Rapid and Sensitive LC-

MS/MS method for the quantification of a nueroprotective

thiazolidinedione ligand, NL-1, in mouse serum". Poster presented at

"Research Day 2012", Cleveland State University.

HONORS

. Certificate of Completion in "New Developments in Clinical Research &

Forensic Toxicology Using LC/MS/MS", 2013, Professional Acknowledgement

for Continuing Education (P.A.C.E.), American Society for Clinical

Laboratories Science.

. Doctoral Dissertation Research Expense Award and Fellowship, 2012-2013,

From Office of Research, Cleveland State University.

. Teaching Assistantship, Department of Chemistry, Cleveland State

University, 2011-Present.

. Research Assistantship, College of Sciences and Health Professions,

Cleveland State University, 2008-2011.

PROFESSIONAL AFFILIATIONS

American Society for Mass Spectrometry (ASMS) 2012 -

Present*

American Chemical Society (ACS) 2012 - Present*

Graduate Student Inter Disciplinary Research Club (GSIRC) 2009 -

Present*

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