Design Project

Jeffrey M. Grimes

** **** **** ***. *** #* Allston MA. 02134

941-***-**** ***********@*****.***

Education

University of Massachusetts at Amherst Amherst, MA Graduation (Degree to be Awarded) –

February 2014

Master of Science in Microbiology

Thesis title: Enhancement of Lateral Flow Assay for Detection of Whole Chlamydia trachomatis

Elementary Bodies

University of Central Florida Orlando, FL Graduation - August 2009

Bachelor of Science in Molecular Biology and Microbiology

Minor History

Work Experience

Umass at Amherst Food Science Department 2011 - Present Graduate Researcher

Under: Dr. Sam Nugen

General Duties:

• Undertook initial study and design on the feasibility of a complete blood count (CBC) micro

fluidic device

• Handled IRB paperwork and approval process for acquisition of Human Blood samples for CBC

device

• Preformed principal design, manufacturing, and testing of nano-fluidic Entropic trapping project.

(clean room, photolithography, and reactive ion etching)

• Preformed principal research and optimization for enhancements of the limit of detection for

Whole Viral and Chlamydial elementary bodies Lateral test flow strip projects

• Maintained Viral and Chlamydial cell culture

• Conjugated Gold, and Fluorescent nano-particle to Antibodies, to act as reporters in Lateral flow

assays

• Ordered and maintained chemical stocks

UCF Chemistry Department 2009 - 2011 Research Associate

Under: Dr. Dmitry Kolpashchikov

General Duties:

• Preformed principal research and optimization of Binary DNA Probes, and explored the effects of

complex hairpin loop structure on hybridization

• Conduct experimental trials on BDP and traditional Molecular beacon systems for Detecting

SNPs

• Design and the theoretical testing of Hybridization probes

• Transformed E.coli for over expression of 16s RNA

• Extracted and maintained 16s RNA samples

• Trained undergraduates in lab procedure and protocol

• Supervised undergraduate researchers

• Ordered and maintained Chemical stock

Jeffrey M. Grimes

39 Park Vale Ave. Apt #4 Allston MA. 02134

941-***-**** ***********@*****.***

UCF Chemistry Department 2007 - 2009 Undergraduate Researcher

Under: Dr. Dmitry Kolpashchikov

General Duties:

• Worked on Binary DNA Probe project, designed DNA probes and optimized conditions to

improve signal to noise rations

• Conduct and designed experimental trials for Binary DNA Probe

• Design and theoretical testing of Hybridization probes

• Ordered and maintained Chemical stocks

Publications

• Grimes, J., Webley, W., Nugen S., Enhancement of Lateral Flow Assay for Detection of

Whole Chlamydia trachomatis Elementary Bodies, In Progress

• Alcaine, S., Grimes, J., Detection and Quantification of E. coli via T7 bacteriaphage as the

reporter, In Progress

• Grimes, J., Alcaine, S., Nugen S., Enhancement of the Limit of Detection of T7

Bacteriaphage via Fluorescent Nano-particles, Submitted

• He, F., Grimes, J., Nugen S., A Hybrid Paper and Microfluidic Chip with Electrowetting

Valves and Colorimetric Detection, Submitted

• Nguyen, C., Grimes, J., Gerasimova, Y. V. and Kolpashchikov, D. M. (2011), Molecular-

Beacon-Based Tricomponent Probe for SNP Analysis in Folded Nucleic Acids . Chem. Eur. J.,

17: 13052–13058. doi: 10.1002/chem.201101987

• Grimes, J., Gerasimova, Y. V. and Kolpashchikov, D. M. (2010), Real-Time SNP Analysis in

Secondary-Structure-Folded Nucleic Acids. Angew. Chem. Int. Ed., 49: 8950–8953.

doi: 10.1002/anie.201004475

Honors

• Article Real-Time SNP Analysis in Secondary-Structure-Folded Nucleic Acids, was selected

for Faculty of 1000

Skills

Lab Skills

• PCR

• Cell culture techniques (Bacteria and Viruses)

• BSL-2 virus and bacteria handling and infection techniques

• Protein extraction and purification (SDS-Page, Size-exclusion chromatography)

• DNA and RNA extraction and purification

• DNA cloning

• Transformation of E.coli

• Microscopy

• Spectrophotometry (UV and IR)

• HPLC

Jeffrey M. Grimes

39 Park Vale Ave. Apt #4 Allston MA. 02134

941-***-**** ***********@*****.***

• PAGE and Agarose Gel electrophoresis (Native and Denaturing)

• ELISA assay

• Western, Northern, and Southern Blot assay

• Handling of radioactive material (Deuterium, P32)

• Photo lithography

• Clean room training

• Auto CAD

• Lateral flow assay, design and assembly

• Micro and Nano Fluidics, design and assembly

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