Project Development

Juying Li, PhD

CONTACT INFORMATION

Juying Li

** ***** **

Arlington, MA 02476

Phone: 617-***-****

Email: ************@*****.***

Status: US permanent resident with green card

QUALIFICATIONS

. Over ten years of experience in molecular and cell biology in academia

and industry.

. Experienced in ELISA, immunoassay systems, mammalian cell tissue culture

and cell-based assay development.

. Mentored graduate students and research associates for projects.

. Superior writing skill with multiple first-author high profile

publications.

. Highly motivated, organized and productive individual with abilities to

work independently and in a team in high-pressure environments.

EDUCATION

HARVARD UNIVERSITY, Cambridge, MA

2001-2007

PhD in Cell and Developmental Biology (Advisor: Prof. Junying Yuan)

Project Key Findings: Characterization of the role of pro-apoptotic protein

caspase-11 in regulating actin cytoskeleton, immune cell migration and

inflammation

. Discovered novel binding proteins of caspase-11 through protein affinity

purification and mass spectrometry.

. Performed in vitro cell migration assays and mouse in vivo homing assay

to validate the novel function of caspase-11 in regulating immune cell

migration in peritonitis model.

. Conducted live cell imaging and quantitative microscopic analysis on the

migration of primary mouse macrophages.

SPECIAL CLASS FOR GIFTED YOUNG, UNIVERSITY OF SCIENCE AND TECHNOLOGY OF

CHINA, Hefei, China

1996-2001

BS in Biology (first rank in the class)

Thesis project: Development of a sandwich ELISA assay using monoclonal anti-

ErbB2/HER2 antibodies for breast cancer prognosis

PROFESSIONAL EXPERIENCE

METAMARK GENETICS, INC. Cambridge, MA

2012-2013

Research Scientist

Key projects: Identification and validation of novel cancer biomarkers and

druggable targets

. Identification of potential druggable targets (small molecular inhibitor

targets and therapeutic antibody targets) through bioinformatics analysis

of melanoma and prostate cancer mouse models.

. Determination of relevant cancer types and cancer progression correlation

for novel targets using immunohistochemistry on human normal and tumor

tissue arrays.

. Loss of function and mechanism of action studies of novel targets in

representative cancer cell lines using cell culture based functional

assays, drug sensitivity assays and oncology signaling pathway analysis.

CUTANEOUS BIOLOGY RESEARCH CENTER, MASSACHUSETTS GENERAL HOSPITAL/HARVARD

MEDICAL SCHOOL 2008-2012

Postdoctoral Research Fellow (Advisor: Prof. David E. Fisher)

Project Key Findings: Characterized transcriptional regulation in skin

pigmentation and melanoma

. Discovered the essential role of transcription factor YY1 in melanocyte

development and melanoma cell survival using conditional knockout mice

and RNAi in cell culture.

. Identified transcription factor target genes of MITF and YY1 using gene

expression arrays, whole genome ChIP-seq and bioinformatics analysis.

. Elucidated the function of novel target genes and pathways downstream of

MITF and YY1 in melanoma.

DEPARTMENT OF CELL BIOLOGY, HARVARD MEDICAL SCHOOL 2007-2008

Postdoctoral Research Fellow (Advisor: Prof. Junying Yuan)

Project Key Findings: Discovered the roles of Flightless-I in caspase

activation, apoptotic cell death and inflammation

. Identified Flightless-I as a novel caspase binding protein through

affinity purification.

. Elucidated the inhibitory effect of Flightless-I on caspase activation,

apoptotic cell death and inflammatory cytokine production using caspase

cleavage assay, cell death assays and cytokine ELISA.

. Investigated actin cytoskeleton and caspase localization using

fluorescence confocal microscope.

. Study of the crosstalk between apoptosis, autophagy and cell cycle

pathways.

PUBLICATIONS

. Li J, Song JS, Bell RJA, Tran TT, Haq R, Liu H, Love KT, Langer R,

Anderson DG, Larue L, Fisher DE. 2012 YY1 regulates melanocyte

development and function by co-operating with MITF. PLoS Genetics.

8(5):e1002688.

. Li J, Yuan J. 2011. Chapter 11 Cell death in nervous system development

and neurological disease, Apoptosis: Physiology and Pathology (textbook),

Cambridge University Press.

Zhao X, Fiske B, Kawakami A, Li J, Fisher DE. 2011. Regulation of MITF

stability by the USP13 deubiquitinase. Nature Communications. 2:414.

Furuya T, Kim M, Lipinski M, Li J, Kim D, Lu T, Shen Y, Rameh L, Yankner

B, Tsai LH, Yuan J. 2010. Negative regulation of Vps34 by Cdk mediated

phosphorylation. Molecular Cell. 38(4):500-511.

. Li J, Yuan J. 2008. Caspases in apoptosis and beyond. Oncogene.

27(48):6194-6206.

. Li J, Yin HL, Yuan J. 2008. Flightless-I regulates pro-inflammatory

caspases by selectively modulating intracellular localization and caspase

activity. Journal of Cell Biology. 181(2):321-33.

. Li J, Brieher WM, Scimone ML, Kang SJ, Zhu H, Yin H, von Andrian UH,

Mitchison T, Yuan J. 2007. Caspase-11 regulates cell migration by

promoting Aip1-Cofilin-mediated actin depolymerization. Nature Cell

Biology (Article). 9(3):276-286. (This work was highlighted on cover and

in "News and views" by Nature Cell Biology, 2007, 9(3): 245-246 and in

"Editor's choice" by Science's STKE 6 March 2007: tw74.)

. Li P, Li Y, Li JY, Liu J. 2003. Characterization and utilization of two

novel anti-erbB-2 monoclonal antibodies in detection of soluble ErbB-2

for breast cancer prognosis. Cancer Letters. 193(2):139-148.

MEETING ABSTRACTS AND PRESENTATIONS

. Li J, Brieher WM, Scimone ML, Kang SJ, Zhu H, Yin H, von Andrian UH,

Mitchison T, Yuan J. Caspase-11 regulates cell migration by promoting

Aip1-Cofilin-mediated actin depolymerization. American Society of Cell

Biology the 46th annual meeting. Dec 2006. Invited oral presentation in

"Apoptosis" mini-symposium.

(Media report highlighting my talk on Journal of Cell Biology, 2007,

176(5): 552-558.)

. Li J, Brieher WM, Scimone ML, Kang SJ, Zhu H, Yin H, von Andrian UH,

Mitchison T, Yuan J. Caspase-11 regulates cell migration by promoting

Aip1-Cofilin-mediated actin depolymerization. Gordon Research Conference

on Cell Death. Sep 2006. Meeting abstract.

SCIENTIFIC SKILLS

Cell Biology

Mammalian cell culture, transfection, selection, generation of stable cell

lines, RNAi (siRNA and shRNA knockdown), flow cytometry (FACS),

immunofluorescence, confocal microscopy, live cell imaging (time-lapse

microscopy), cell proliferation and cell death assays, cytokine production

assay, cell migration and invasion assays, in vitro enzyme assays, signal

transduction pathway analysis, reporter-gene assays, ELISA, retrovirus and

lentivirus production, viral infection

Molecular Biology

DNA/RNA isolation and purification, gel electrophoresis (including 2-D gel,

IEF and SDS-PAGE), molecular cloning (including retroviral and lentiviral

vector development and cloning), cDNA synthesis, site-directed mutagenesis,

quantitative real-time PCR, western blotting, immunoprecipitation,

bacterial/mammalian protein expression and purification, chromatin

immunoprecipitation (ChIP)

Mouse study

Mouse crossing, genotyping, injection, tumor xenograft, isolation of

splenocytes, bone marrow and peritoneal macrophages, tissue dissection and

cryoperservation, histology

AWARDS AND HONORS

. Star Award, Metamark Genetics Inc, 2012

. NIH Dermatology Training Grant (2T32AR007098-37), 2012

. Tosteson Postdoctoral Fellowship, Massachusetts General Hospital, 2009-

2010

. Graduate Student Fellowship (Fu Fellowship), Harvard University, 2001-

2006

. Bao Gang Education Scholarship, Bao Gang Education Foundation, 2000-

2001

REFERENCES

. Available upon request

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