Project Manager Maintenance

JACKINGSON CELESTIN

** **** ****** *****, ***** Lancaster, M A 01561, PO BOX 521 .

Cell: 978-***-**** . ***********@*****.***

Professional Summary

Biologist seeks to improve his scientific skills by performing accurate

research and complying with all laboratory safety protocol and standards.

Education

Bachelor of science in Biology

May 2013

Fitchburg State University, Fitchburg Massachusetts (USA)

Bachelor of science in Agriculture and environment June

2005

University Quisqueya, Port-au-Prince Haiti (W.I)

SUMMARY

. Molecular Biology: In vitro transcription, DNA, RNA, Plasmid isolation

and purification, DNA amplification (PCR), primers design, ligation,

bacteria transformation, gel electrophoresis. Use of autoclave and

incubator.

. Cell culture: Cell line characterization, mammalian cell line

maintenance, preparation and sterilization of media, subculture and

cell line.

. Microbiology: aseptic techniques, media culture, selective and

differential media, bacteria identification techniques Northern, and

western blot.

. Entomology general: Insects anatomy and physiology, characterization

and identification of insects

. Organic Chemistry: Gas Chromatography analysis (G.C), Mass

spectrometry (M.S), IR spectroscopy, qualitative organic analysis.

. Tools: Good knowledge in Microsoft office in general

. Language spoken: English and French

Work Experience

Special Aid teacher for people living with disability, 07/2013 to present

State of Massachusetts

Currently on a daily basis i?m teaching people living with disability basic

life skills which involved; how to respond to people, how to behave in

public, how to make choices, how to make friends, how to manage their

friendship, etc.

Internship, 06/2012 to 08/2012

Novartis, Cambridge, MA

The program was a ten weeks program from June to August 2012. The program

provided internships in basic laboratory research related to gene therapy

of cystic fibrosis.

. Mammalian cell culture (media preparation, cell count, plate reading

and cell maintenance).

. DNA isolation of cystic fibrosis from heterozygous patient with a

milder form of the disease using instagene matrix and protease

enzymes.

. Site-directed mutagenesis on cells with a normal copy of the CTFR gene

. CFTR gene amplification using PCR and gel electrophoresis.

. In vitro transcription and DNA purification

. RNA interference, gene injection using some vector techniques

. Mouse manipulation

Academic Projects

RNA interference in C. elegans, targeted genes BCR-1, from January-

May 2013: The goal was to produce a functional knock out of the BRC-1 gene.

. Gene identification using computational biology software tools

. Media preparation, gene isolation using appropriate primers and

DNA amplification by PCR

. Extraction and purification of DNA from gel electrophoresis

. Ligation of gene of interest into plasmid, transformation of

competent E. coli

. RNA interference

. E. coli and C. elegans culture

RNA interference in Drosophila melanogaster September 2011-November 2011:

the goal was to understand the mechanism of gene expression and regulation

in Drosophila melanogaster.

. Isolation of DNA from flies and primers determination using

computational biology software tools (flybase.org)

. Amplification of DNA by PCR

. Gel electrophoresis and purification of PCR products

. In vitro transcription

. Production in vitro of double stranded RNA

. Purification of the double stranded RNA

Incidence of Mildew (root burn disease) on the production of Potatoes

Solanum tuberosum, Savane Zombie (Haiti) May- July 2007: The goal of this

project was to analyze the impact of the Mildew on the production of

Potatoes in some farms located in the southern part of Haiti. The

population was approximately 30 farmers spread throughout the area,

biostatistics tools were used to interpret the data and appropriate

recommendations were made according to the results of the experiment.

Other

Development project manager, 01/2007 to 01/2009

PADF/USAID, Haiti

In this position, I was a project manager of a development project in the

southern part of Haiti for a US non-governmental organization. I was

basically managing the daily operations of all the aspects of the projects

that were under execution. I was also involved in managing, scheduling, and

training staff members.

Awards

Presentation in the Forth Annual Undergraduate Conference (Fitchburg State

University) May 2013

Presented Interference of C. elegans BRC-1 Genes Using dsRNA Plasmids.

Reseachered interference of C. elegans using transformed dsRNA bacteria by

ingestion.

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