Project Manager Medical

LONG CUI

**A Reinman Road

Warren, NJ *****

Home: 732-***-**** *********@*****.*** Cell: 908-***-****

SUMMARY

Highly motivated Biological Research Scientist with many years experience

in in vivo studies in academy and pharmaceutical industry; effective at

establishing and performing experiences; demonstrated leadership,

reliability, responsibility and efficiency in supporting drug discovery and

drug development; good communication skills to present research findings.

Extensive experience in molecular biology, tumor biology, immunology and

metabolic diseases.

PROFESSIONAL EXPERIENCE

JACKSON LABORATORY-WEST, Sacramento, CA

2/2013 -

Research Associate/Project manager - In Vivo, Cancer

Manage and perform multi-projects including PDX (patient derived xenograft)

projects. Set up and monitor different oncology and PDX models. Manage and

transfer different patient tumors on immunodeficient mice (nude and NSG

mice). Test the efficacy of different chemotherapy compounds and drugs on

tumor models. Coordinate in vivo and in vitro screens for biomarker study.

Plan and schedule in vivo and in vitro study projects.

MERCK RESEARCH LABORATORIES/

SCHERING-PLOUGH RESEARCH INSITUTE, Kenilworth, NJ 2001 - 12/2011

Scientist II - Respiratory and Immunology (2006 - 12/2011)

Scientist I - Dept. of Immunology (2001 - 2006)

Contributed to the team on disease mechanism study, compound screening and

drug discovery and development by in vivo, ex vivo, in vitro and

histological experiments. Adhered SOPs and GLP in all studies.

. Established, validated and conducted different in vivo disease models to

perform mechanism study or evaluating compounds on regular or transgenic

rodent animals; implemented compound administrations and disease scores

scanning, measuring and recording.

. Performed PK/PD and toxicological studies from formulation of compounds,

dose calculations to blood samples collection from mice and rats.

. Collected tissues from different places (mesenteric lymph nodes,

mesenteric fat pad, epididymal fat pad, spleen, liver, gastrocnemius

muscle, pancreas and CNS etc.) for the following in vitro/ex vivo and

histological studies.

. Harvested animal primary immune cells from mouse lung lavage, spleen,

lymph nodes, fat and CNS tissues; Performed Flow Cytometry for

characterization analysis. Quantitative data showed different immune

cells distribution in the lesion and compounds efficacy.

. Maintained cell and tissue culture for cell-based assays using many human

cell lines or cells collected from animals. Performed cell-based assays

on testing small and large molecule inhibitors or agonists for mechanism

study and drug discovery.

. Independently designed, developed and performed tissue lysate, MSD,

ELISA, Luminex, gene transfection, siRNA transfection, MACS, Flow

Cytometry Analysis and antibodies assays for biomarker and gene

expression assessments.

. Performed histological studies on mouse skin, paw, spleen, CNS tissues

(brain and spinal cord) etc; taking pictures of slides and quantitative

image analysis. Performed IHC and contributed pictures to paper

publication.

. Completed all annual Comparative Medicine and Laboratory Animal Resources

(LAR) - Institutional Animal Care & Use Applied trainings; trained junior

scientist on in vivo and in vitro techniques and helped them on trouble

shooting.

. Analyzed all data independently, wrote protocols, amendments and data

reports for all studies to supervisor and program teams both on time or

ahead of schedule, followed up and designed subsequent experiments. Kept

good scientific record on all studies.

. Made literature searching, met with supervisor or project groups to

evaluate progress on programs; made trouble shooting and adjusted study

strategy. Made presentation on regular group meeting, journal club and

department's collaboration meeting.

NYU MEDICAL CENTER, New York, NY 1997 - 2001

Research Associate - Medicine Department (1998 - 2001)

Research Associate - Urology Department (1997 - 1998)

. Involved in several projects for oncology studies on Wnt and NFkB

pathway; evaluated the antitumor potential from pathway inhibition. Made

important contribution on publication.

. Maintained different mammalian cell culture for studies; performed cell

based assays, molecular biology (Cells lysate, Western blot, PCR, RT-PCR

and gel shift analysis etc.) and histological analysis for project

studies.

. Successfully established and performed xenograft models (tumor lung

metastasis model, bone metastasis model and subcutaneous tumor growth

model etc.) for different studies on primary immunodeficient mice.

. Managed lab research animals, performed mouse handling, dosing and tumor

measurement etc. until necropsy and tumor tissues collection for in vitro

and histological studies.

. Performed mouse genomic DNA analysis and protein assays (southern blot

and western blot) to identify gene and protein expression.

. Searched publications; validated methods and protocols for different

studies.

. Provided technique support for other staff in research lab on in vivo and

bench experiments.

. Regularly reported and discussed data in group and had presentation in

department meetings.

EDUCATION

Medical Degree, Medicine. Capital University of Medical Sciences, Beijing,

China

RESEARCH SKILLS AND TECHNIQUES

In VITRO: Sectioning animal tissues (paraffin and frozen tissues), Regular

histological staining, IHC staining, In situ hybridization, Using

microscope, Taking pictures from microscope and performing quantitative

image analysis. Cell and tissue culture, mouse immune cell and human PBMC

isolation and culture, T cell isolation from human blood, Cell preparation

for RNA assay, Protein assay, MSD, ELISA, Luminex, Western blot, Southern

blot, PCR, RT-PCR and agarose gel electrophoresis, Gene transfection, siRNA

transfection, Cell proliferation and migration, Immunofluorescence,

Immunoprecipitation, PK/PD analysis, Nitric Oxide assay, TUNEL assay, Advia

assay, Flow cytometry assay (including cells labeling and preparative FACS;

HTS; Calibur maintenance).

In VIVO: Animal management (include mouse, rat, hamster, guinea pig),

Handling and drug administration on rodents; Set up animal models (PDX and

Tumor cell xenograft models on mice, patient tumor tissue implantation on

mice, abdomen infection model, EAE model, IPS model, Diabetes models,

Adoptive transfer diabetes model, Diet-obese model, Psoriasis model etc.),

Rodent necropsies, Small animal surgeries, Mouse bone derived cell culture,

Rodent intracardiac injection, Animal dosing (IP, PO, IV, IM, SC and

intradermal), Rodent blood collection (tail vein, retro-orbital sinus or

cardiac puncture), Mini-pump on rodent models, Mouse pancreas perfusion,

Animal tissues collection, Collecting and analyzing CSF from rat icv model.

ACHIEVEMENTS AND AWARDS

2001-2003, 2006-2009, 2011 Excellent Awards /Shinning Awards of Shering-

Plough or Merck.

COMPUTER SKILLS

Worked with Provantis, Prism, Microsoft Word, Excel and PowerPoint, Image

Quant, ACT -1 program, FlowJo and Internet for data analysis, scientific

graphic design, and other research activities.

PUBLICATIONS

1. Chung-Her Jenh, Mary Ann Cox, Long Cui, Eva-Pia Reich, Lee Sullivan, Shu-

Cheng Chen, David Kinsley, Shiguang Qian, Seong Heon Kim, Stuart

Rosenblum, Joseph Kozlowski, Jay S Fine, Paul J Zavodny and Daniel

Lundell. A selective and potent CSCR3 antagonist SCH546738 attenuates the

development of autoimmune diseases and delays graft rejection. (BMC

Immunology 2012, 13:2)

2. Stanton MC, Chen SC, Jackson JV, Rojas-Triana A, Kinsley D, Cui L, Fine

JS, GreenfederS, Bober LA, Jenh CH. Inflammatory Signals shift from

adipose to liver during high fat feeding and influence the development of

steatohepatitis in mice. (J Inflamm (Lond). 2011 Mar 16; 8:8.)

3. Engstrom LW, Bober L, Chen SC, Fine JS, Li Y, Stanton MC, Kinsley D, Cui

L, Jackson JV, Rojas-Triana A, Lundell D, Laverty M, Gustafson EL, Jenh

CH, Kowalski TJ, Manfra DJ. Kinetic assessment and therapeutic

modulation of metabolic and inflammatrory profiles in mice on a high-fat

and cholesterol diet. (PPAR Research 2010; 2010:97164. Epub 2010 Apr 22.)

4. Wang Y, Cui L, Gonsiorek W, Min SH, Anilkumar G, Rosenblum S, Kozlowski

J, Lundell D, Fine JS, Grant EP. CCR2 and CXCR4 regulate peripheral

blood monocyte pharmacodynamics and link to efficacy in experimental

autoimmune encephalomyelitis. (J Inflamm (Lond). 2009 Vov 11; 6:32.)

5. Eva-Pia Reich, Long Cui, Lily Yang, Catherine Pugliese-Sivo, Andrei

Golovko, Mary Petro, Galya Vassileva, Inhou Chu, Amin Nomeir, Li-Kang

Zhang, Xian Liang, Joseph A. Kozlowski, Satwant K. Narula, Paul J.

Zavodny and Chuan-Chu Chou. Blocking Ion Channel KCNN4 Alleviates the

Symptoms of Experimental Autoimmune Encephalomyelitis in Mice (European

Journal of Immunology Volume 35, Issue 4, Pages 1027 - 1036

6. Yixing Jiang, Long Cui, Ting-An Yie, Hua Cheng, William N. Rom, Theodore

C. Lee and kam-Meng Tchou-Wong Inhibition of Transformation of Human

Lung Carcinoma Cells by I?B? (Oncogene. 2001 Apr. 26; 20(18)2254-63

"bstract presented at ATS Canada 2000)

7. Olubunmi Afonja, Takashi Shimamoto, John E. Smith, Jr., Long Cui, and

Kenichi Taheshita Use of antisense oligonucleotides to study homeobox

gene function Gene Therapy and Molecular Biology Vol 3, page 281-291,

August 1999

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