Quality Assurance Project

NIKHIL KHATWANI

EDUCATION

Northeastern University, Boston, MA August 2013

Master of Science in Biotechnology (Process Developmental Track)

GPA: 3.5/4.0

Amity University, Noida, India August 2010

Bachelor of Technology in Biotechnology

GPA: 3.7/4.0 - Recipient of Amity University Academic Scholarship

WORK EXPERIENCE

Protein Engineering and Antibody Technologies, EMD Serono Research Institute, Billerica, MA

Research Assistant Co-op January - June

2013

• Managed the Protein Engineering automation lab with equipment’s like Caliper, Biotek and Epmotion

• Operated automated liquid handling systems for high throughput target screening (HTS) along with

developing new protocols to screen for monoclonal antibodies based on different attributes

• Screened for antibodies for ligand based receptors involved in therapeutic purposes to support research and

production

• Routinely executed immunoassays towards supporting lead candidate discovery phase in a GLP

compliant environment

• Planned, executed, validated and analyzed experimental designs for homogenous assays, ELISA -

competition, binding, quantitation, functional screens and HTRF-FRET

• Developed HTRF- FRET optimized methods

• Performed troubleshooting for complex technical and scientific problems

• Documented experimental and scientific data for internal distribution using OpenLab Kalabie ELN

• Studied, followed, created and modified SOP’s in compliance to in house quality system regulations

• Optimized experimental parameters facilitating improved consumables storage and experimental results

• Collaborated efficiently with supervisor and group members to meet project deadlines and presented

scientific data to support ongoing research

• Performed routine equipment maintenance and calibration and actively involved in laboratory space organization

Harvard Medical School, Microbiology & Immunology Division, Boston, MA

Research Intern May – October

2012

• Performed experiments to better understand, define new subsets of Natural Killer cells based on their origin

• Conduct Flow Cytometry to differentiate between antigens and to exploit their unique abilities in novel treatment

options for patients suffering from autoimmune diseases, viral infections and tumors

• Performed Weekly Weaning and Genotyping of mice

• Immunized and dissected Mice with H1N1, along with organ prepping and sorting of cells

• Planned and executed a 3 week weight loss study on Swine Flu infected Mice

• Conducting PCR, SDS PAGE, Western Blotting and cell culturing with minimal expertise

• Performed Confocal Microscopy for Tumor Study

Biocon International Limited, Quality Assurance Department, Bangalore, India

Project Intern May – December

2010

• Worked in the Quality and Assurance department to prepare reports on Anti cholesterol drugs, involving

Atorvastatin, Fluvastatin and Rosuvastatin

• Used analytical methods on Anti Cholesterol drugs to test for various parameters as per consumer requirements

• Developed new methods used in downstream processing of monoclonal antibodies

• Performed LC-MS, HPLC, UPLC, GC, IR, UV Spectroscopy, Western blotting, SDS PAGE, PCR and

Polarimetery

Indian Army Hospital –Immunology Department, New Delhi, India

Project Intern March 2008 -

June 2009

• Examined patient’s urine and saliva samples for various illnesses and performed Cell Culture techniques

• Conducted Water sampling throughout the hospital by testing them for presence of microorganisms

• Performed a project on validation of 8 microorganisms over a 1 month period to better understand their

properties

• Monitored Immunized rodents for behavioral studies using software tools with minimal supervision

COMPUTER SKILLS

MS Office, SPSS, C, C++, SQL, DBMS, BLAST, Genedata, Graphpad, Gen5, Ilink Pro

INTERESTS

Badminton, Professional Snooker/Pool, Cricket and Soccer

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