Customer Service Engineer
Resume:
DMITRI PROUDNIKOV, Ph. D.
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**** ***** ******, ***. *** Corona, NY, 11368 (willing to relocate)
Tel.: 917-***-****
Chemist, Molecular Biologist
Patent applications/ Technical writing/ Scientific project management/
Organic synthesis / Molecular biology/ Genetics/ Biotechnology/ Method
development/ Science liaison
By leading projects in cross-functional teams in
academic/clinical/industrial collaborative settings, I have built novel
methodologies in biotechnology, synthesized novel drugs and made
discoveries in clinical applications of human genetics. I have been
described as a result oriented self-starter with proven problem-solving
skills. I wish to be a part of a company that offers a positive atmosphere
of learning, working under a management of inspiring minds that would make
effective use of and groom my skills and creativity.
.Produced intellectual property in collaboration with industrial
partnership, was paid for commercial use of patents
.Designed and synthesized novel antiretroviral drugs
.Designed novel biotechnology methods; used these methods for pathogen
detection and analysis
.Led studies in human genetics, addiction and HIV; provided oversight
of clinical data analysis
.Wrote 28 publications (patents, research papers, book chapters);
presented talks at conferences
SKILLS SUMMARY
Experiment design
Data analysis/interpretation
In technical writing:
Research paper, Textbook Patent, Grant, Presentation
Operation of:
HPLC, MS, array scanners, ABI 394, ABI 3730xl, etc.
In synthetic chemistry:
Design/performance of
multiple step synthesis
Product isolation, analysis
Oligonucleotide synthesis
UV, IR, FL, NMR, MS
Chromatography: RP, IE, SE, silica, LP, HPLC, TLC
In molecular biology:
Clinical sample handling
Assay development
High throughput screening
DNA/RNA isolation/analysis
Gene expression
DNA/RNA analysis software
In genetics:
Statistics, LD analysis
Association with disease
Clinical data analysis
Interpersonal skills:
Multitasking team player
Meets deadlines
Eager to learn
Creative, analytical, flexible
Customer service experience
English/Russian
SELECTED ACCOMPLISHMENTS
Synthesized novel antiretroviral drugs. To be an effective drug, a compound
needs to have low toxicity and be able to be recognized effectively by
viral reverse transcriptase. Synthesized novel drugs, lixo- and
riboepithiothymidines that showed low toxicity and high antiretroviral
properties
Developed a new methodology for fluorescent labeling and immobilization of
nucleic acids (US patented). Polyacrylamide based microarrays require use
of fragmented DNA fluorescently labeled at terminal position. Developed a
novel reliable methodology for DNA fragmentation with simultaneous
incorporation of fluorophore in each fragment. Similarly, developed a
methodology for DNA immobilization on microarrays.
Developed a new methodology for quantification of dangerous mutants in
viral vaccines. Polio and yellow fever viruses have high rate of mutation,
some mutants are extremely dangerous and need to be screened for in
vaccines. Developed fast, inexpensive and reliable method that allows
quantifying as low as 5% of mutants (teamed with FDA); alternative
techniques allow quantification of 50% of mutants
Developed a new methodology for molecular haplotype analysis (allelic
allocation of gene variants). Haplotypes may provide better understanding
of genetic basis of complex disorders compared to genotypes, but are more
difficult to evaluate. Developed a novel method for molecular haplotype
analysis that is available for high throughput use, faster, less expansive
and more reliable than alternative methods
DMITRI PROUDNIKOV, Ph. D.
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CAREER HISTORY
The Rockefeller University (RU), New York, NY
2001 - Jan 2013
Research Associate, The Laboratory of the Biology of Addictive
Diseases
Led projects targeting gene expression, human genetics, addiction, HIV
progression and biotechnology; teamed with clinicians and statisticians
planning and performing experiments and providing general oversight;
submitted grant proposals as a PI; wrote manuscripts on genetics,
alcoholism, addiction and biotechnology.
Selected achievements
2008 - 2013
.Genetic makeup influences response to therapy. Identified gene
variants changing response to HIV treatment (HAART) using the world's
largest female cohort (3000 subjects) collected for study of HIV clinical
history: application for personalized medicine. Identified ACTH receptor
variants changing stress responsivity.
2004 -
2009
.Genetic makeup dictates predisposition for complex disorders.
Identified gene variants influencing vulnerability to addiction; reviewed
and corrected data analysis performed by statistical team.
2001 -
2006
.Led studies that required hands-on experience in molecular biology:
gene expression in human blood and animal tissues, methylation patterns,
molecular haplotyping.
Argonne National Laboratory (ANL), Argonne, IL, USA
1996 - 2000
Chemist, Visiting Scientist, Biosciences Division
Joined ANL being a Ph.D. student at Engelhardt Institute of Molecular
Biology (Moscow, Russia)
Led development of the GENERIC array production line (published);
performed troubleshooting, oversight of the array components quality and
overall product performance. Performed custom synthesis of commercially
unavailable fluorophores and other compounds. Developed novel methods in
biotechnology (US patents #6,465,174 B1 and #5,981,734), currently are in
commercial use by US companies. Developed novel methods for detection of
pathogenic bacteria and quantification of mutants in vaccines.
Samara State University, Samara, Russia
1988- 1993
Research Assistant (1988-1991), Chemist-engineer (1991-1993),
Department of Organic Chemistry
Designed and synthesized modified sugars and nucleotides as novel
antiretroviral drugs (anti-HIV) or their precursors; was acknowledged for
synthesis of novel compounds in manuscripts.
EDUCATION
Engelhardt Institute of Molecular Biology of Russian Academy of Sciences
(Moscow, Russia) 1993- 1998
A part of the project was done in Argonne National Laboratory (Argonne, IL,
USA)
Ph.D. in Chemistry; thesis: "Hybridization of labeled probes on
microarrays"
Samara State University (Samara, Russia)
1986- 1991
B.S./M.S. in Chemistry and Teaching (dual major); thesis: "Synthesis of
epoxythymidine and its analogs"
OTHER
.Member of the New York Academy of Sciences
.Driver license; NIH Human Subject Protection Certificate
.Upon editor's request, reviewed manuscripts submitted for journals:
Nucleic Acids Res., Anal. Biochem., Biotechniques, J. Neurosci. Methods,
Biol. Psych., Pharmacogenet. Genom.
DMITRI PROUDNIKOV, Ph. D.
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LIST OF PUBLICATIONS
US PATENTS
1. Mirzabekov A.D., Yershov G.M., Guschin D.Y., Gemmell M.A., Shick
V.V., Proudnikov D.Y., Timofeev, E.N. Rapid method to detect duplex
formation in sequencing by hybridization methods, a method for constructing
containment structures for reagent interaction. United States Patent
#6,465,174 B1; 2002.
2. Mirzabekov A.D., Proudnikov D.Y., Timofeev; E.N, Kochetkova; S.V.,
Florentiev V.L., Shick; V.V., Methods for immobilizing nucleic acids on a
gel substrate. United States Patent # 5,981,734; 1999.
MANUSCRIPTS
3. Proudnikov D, Mirzabekov A. Chemical methods of DNA and RNA
fluorescent labeling. Nucleic Acids Res, 1996; 24:4535-4542
4. Guschin D, Yershov G, Zaslavski A, Gemmel A, Shick V, Proudnikov
D, Arenkov P, Mirzabekov A. Manual manufacturing of Oligonucleotide, DNA,
and Protein Microchips. Anal Biochem, 1997; 250:203-211.
5. Guschin DY, Mobarry BK, Proudnikov D, et al. Oligonucleotide
Microchips as Genosensors for Determinative and Environmental Studies in
Microbiology. Appl Environ Microbiol, 1997;63:2397-402.
6. Fotin AV, Drobishev AL, Proudnikov DY, et al. Parallel
thermodynamic analysis of duplexes on oligonucleotide microchips. Nucleic
Acids Res, 1998; 26:1515-1521.
7. Proudnikov D, Timofeev E, Mirzabekov A. Immobilization of DNA in
Polyacrylamide gel for the Manufacturing of DNA and DNA-Oligonucleotide
Microchips. Anal Biochem, 1998; 259:34-41.
8. Proudnikov D, Kirillov E, Chumakov K, et al. Analysis of mutations
in Oral Poliovirus Vaccine by hybridization with oligonucleotide
microchips. Biologicals, 2000; 28:57-66.
9. Chechetkin VR, Turygin AY, Proudnikov DY, et al. Sequencing by
hybridization with the generic 6-mer oligonucleotide microarray: an
advanced scheme for data processing. J Biomol Struct Dyn 2000; 18:83-101.
10. LaForge KS, Shick V, Spangler R, Proudnikov D, et al. Detection of
single nucleotide polymorphisms of the human mu opioid receptor gene by
hybridization or single nucleotide extension on custom oligonucleotide
gelpad microchips: potential in studies of addiction. Am J Med Genet, 2000;
96:604-15.
11. Proudnikov D, Yuferov V, Zhou Y, et al. Optimizing Primer-Probe
Design for Fluorescent PCR. J Neurosci Methods 2003; 123:31-45.
12. Proudnikov D, Yuferov V, LaForge KS, et al. Quantfication of
multiple mRNA levels in rat brain regions using real time optical PCR.
Brain Res, Mol Brain Res 2003; 112:182-185.
13. Proudnikov D, LaForge KS, Kreek MJ. High-throughput molecular
haplotype analysis (allelic assignment) of single-nucleotide polymorphisms
by fluorescent polymerase chain reaction. Anal Biochem 2004; 335:165-167.
14. Bart G, Kreek MJ, Ott J, LaForge KS, Proudnikov D, et al.
Increased attributable risk related to a functional mu-opioid receptor gene
polymorphism in association with alcohol dependence in central Sweden.
Neuropsychopharmacology 2005; 30:417-422.
15. Proudnikov D, LaForge KS, Hofflich H, et al. Association analysis
of polymorphisms in serotonin 1B receptor (HTR1B) gene with heroin
addiction: a comparison of molecular and statistically estimated
haplotypes. Pharmacogenet. Genomics 2006; 16:25-36.
16. Nielsen DA, Barral S, Proudnikov D, et al. TPH2 and TPH1:
Association of Variants and Interactions with Heroin Addiction. Behav Genet
2008; 38:133-50.
17. Oosterhuis BE, LaForge KS, Proudnikov D, et al. Catechol-O-
methyltransferase (COMT) gene variants: Possible association of the
Val158Met variant with opiate addiction in Hispanic women. Am J Med Genet B
Neuropsychiatr Genet 2008; 147B:793-8.
18. Proudnikov D, Hamon S, Ott J, Kreek MJ, Association of
polymorphisms in the melanocortin receptor type 2 (MC2R, ACTH receptor)
gene with heroin addiction. Neuroscience Letters 2008; 435:234-239.
19. Proudnikov D, Kroslak T, Sipe JC, et al. Association of
polymorphisms of the cannabinoid receptor (CNR1) and fatty acid amide
hydrolase (FAAH) genes with heroin addiction: impact of long repeats of
CNR1. Pharmacogenomics J 2010; 10:232-42.
DMITRI PROUDNIKOV, Ph. D.
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20. Briant JA, Nielsen DA, Proudnikov D, et al. Evidence for
association of two variants of the nociceptin/orphanin FQ receptor gene
OPRL1 with vulnerability to develop opiate addiction in Caucasians.
Psychiatr Genet 2010; 20:65-72.
21. Yuferov V, Levran O, Proudnikov D, et al. Search for genetic
markers and functional variants involved in the development of opiate and
cocaine addiction and treatment. Ann NY Acad Sci 2010; 1187:184-207.
22. Zhou Y, Proudnikov D, Yuferov V, Kreek MJ. Drug-induced and
genetic alterations in stress-responsive systems: Implications for specific
addictive diseases. Brain Res 2010; 1314:235-52.
23. Nielsen D, Proudnikov D, Kreek MJ. Genetics of Impulsivity. In:
J.E. Grant and M.N. Potenza, ed., The Oxford Handbook of Impulse Control
Disorders. New York. Oxford University Press. 2011; 217-31.
24. Proudnikov, D, Yuferov, Y and Kreek, MJ. Detecting polymorphisms
in G protein-coupled receptor genes. In: C.W. Stevens, ed., Methods for
the Discovery and Characterization of G Protein-Coupled Receptors, New
York: Springer, 2011; 35-47.
25. Proudnikov D, Randesi M, Levran O, et al. Association of
polymorphisms of the mu opioid receptor gene with the severity of HIV
infection and response to HIV treatment. J Infect Dis 2012; 10:232-42.
26. Kreek MJ, Proudnikov D, Nielsen D, et al. Human molecular genetics
of opioid addiction. In: WH Berrettini, J Nurnberger ed., Principles of
Psychiatric Genetics. Cambridge University Press 2012; 297-306.
27. Proudnikov D, Yuferov V, Randesi M, Kreek MJ. Genetics of Opioid
Addiction. In: P. M. Miller, ed., Encyclopedia of Addictive Behaviors,
Elsevier, Oxford, 2012 (In press).
28. Proudnikov D, Randesi M, Levran O, et al. Polymorphisms of the
kappa opioid receptor and prodynorphin genes: HIV risk and HIV natural
history. J Acquir Immune Defic Syndr, in press, 2013.
INVITED TALKS
1. Genotype and molecular haplotype analyses of serotonin 1B receptor
gene by fluorescent PCR: Molecular implications to drug addiction. 67th
Annual Scientific Meeting of The Collage on Problems on Drug Dependence,
Orlando, FL, June 18-23 2005.
2. Massive analysis of gene expression by TaqMan in application to
neurobiological studies. 33-d International Narcotics Research Conference.
Asilomar, Pacific Grove, CA. July 9-14, 2002.
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