Assistant Project

Siavash Mostafavi

** ********* ***, ******, ** **459

ab9iiq@r.postjobfree.com

617-***-****

Profile

Biochemist speciallizing in enzymology and structural biology

Demonstrated expertise with enzyme kinetics, purification, and

crystallization

Highly familiar with small molecule, pharmaceutical and physical chemistry

Experienced with the use, maintainance and repair of the AKTA FPLC

Proficient in PCR, PAGE, western blots, bacterial/mamallian cell culture,

IHS, ELISA

Education

(M.S) Biological Sciences - Biotechnology, GPA 3.55

University of Massachusetts Lowell, Lowell, MA, May 2013

(B.S) Biological Sciences - Biotechnology, GPA 3.13

University of Massachusetts Lowell, Lowell, MA, May 2012

Related Experience

Master's Project, UMass Lowell, January 2013-May 2013

Expressed and purified multiple mutants of the enzyme Glycosylasparaginase

using affinity purification

Further purified the enzyme by the AKTA FPLC (SE/IE)

Biochemically characterized the kinetics of the enzyme mutants enzyme using

assays

Teaching Assistant, UMass Lowell, January 2013-May 2013

Instruct and grade biology lab classes for non-science majors

Research Assistant, UMass Lowell, Lowell, MA, May 2011-present

Planned, executed and analyzed molecular biology tasks such as plasmid

isolation, cloning, transformation, expression and purification of proteins

Conducted thousands enzyme assays to screen for potential drugs

Screened for conditions of crystallization for various proteins, as well as

handling, freezing, and mounting of the crystals inside an X-ray machine

Trained incoming graduate students in the above tasks

Visiting Collaborator, Broad Institute, Cambridge, MA, November-December

2011

Purified large quantities of enzyme (Affinity/FPLC)

Developed optimal assay conditions

Set up equipment for High throughput screening of small molecules for

potential drug activity

Publications

"Hypomethylation of the serotonin receptor type-2A Gene (HTR2A) at T102C

polymorphic site in DNA derived from the saliva of patients with

schizophrenia and bipolar disorder.", Ghadirivasfi M, Nohesara S,

Ahmadkhaniha HR, Eskandari MR, Mostafavi S, Thiagalingam S, Abdolmaleky HM,

American Journal of Medical Genetics, Part B. July 2011, 6B(5):536-45

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