Project Data

NIRAVKUMAR PATEL

* ******** **, ***#*, Milford, MA, 01757 ~ ***********@*****.*** ~ 551-***-****

PROFESSIONAL SUMMARY:

- 5+ years of experience in cancer cell biology and molecular biology

- Proficient in designing cell based assays in vitro and efficacy studies in vivo

- Expertise at operating instruments: BD FACSCalibur flow cytometer, size and zeta analyzer, plate

readers, UV/VIS photospectrometer, confocal microscope, and HPLC (DAD/Fluorescence detector,

exposure to MS)

- Experienced formulating antibodies or peptide-modified lipid-based polymeric nanoparticle systems

with small molecules and nucleic acids

- Performed literature search, data interpretation, data presentation and published research articles

EDUCATION:

Northeastern University, Boston, MA

Ph.D., Pharmaceutical Sciences and Drug Delivery Systems Apr 2013

Master of Science (Thesis), Pharmaceutical Sciences and Drug Delivery Systems Dec 08

Veer Narmad South Gujarat University, India

Bachelor of Science, Pharmacy May 05

SKILLS:

Cell Culture: Routinely worked with various mammalian cancer cell lines such as SKOV-3, SKOV-3TR,

MCF-7, MCF-7/ADR, A2780, 2780AD, HeLa and non-cancerous cell lines NIH-3T3

Cell based assays: Performed proliferation assays, GFP transfection using lipofectamine, gene silencing by

siRNA, antibody production and purification, cell cycle analysis, colorimetric assays

Animal Handling: Developed tumor xenografts in rodents, administered drugs via IV and IP injections,

performed bio-distribution, isolated various organs and performed histology

Lab techniques: Western Blot, ELISA, HPLC, Gel electrophoresis, colorimetric assays, fluorometric assays,

confocal microscopy, and FACS

Delivery Systems: Lipid based nanoparticles such as liposomes and micelles

Computer skills: MS office (Word, PowerPoint and Excel), SPSS and Graphpad Prism (statistical analysis

software)

WORK EXPERIENCE:

RESEARCH ASSOCIATE, Center for Pharmaceutical Biotechnology and Nanomedicine, MA Sep 10

- Apr 13

- Managed multiple projects listed below as well as supervised thesis work for an MS student

- Performed literature search, designed experiments, wrote SOPs, and generated quality data

- Provided initial instrument operating training to laboratory technicians

- Performed statistical analysis and analyzed data

- Presented data at scientific meetings as well as published original articles in scientific journals

Project 1: Three dimensional spheroid culture: Evaluation of cancer cell behavior.

- Optimized protocols for growing micro tumor tissues in 3D using spheroid culture

- Studied cell cycle stages and cellular responses upon treatment with chemotherapeutic agents

- Evaluated penetration of nanoparticle formulations using confocal microscopy

- Prepared 2C5-modified doxorubicin-loaded micelles to evaluate nanoparticle accumulation and studied

the effects of surface modification on physiological barriers presented by solid tumors

Project 2: Combination therapies for the reversal of the multidrug resistance in vitro and in vivo.

- Preparations: Tariquidar- and paclitaxel-loaded liposomes

Curcumin- and paclitaxel-loaded micelles

Curcumin-and doxorubicin-loaded micelles

- Characterization: Size distribution, zeta potential, Antibody activity using ELISA

Loading efficiency using HPLC

- Cell based assay: Cell association using FACS

IC50 value for the reversal of the MDR

- Animal study: Antitumor activity in nu/nu mice, Bio-distribution studies

Sample preparation from blood and tissues like liver, kidney, brain and tumor

Project 3: Production and purification of nucleohistone-specific monoclonal antibody 2C5

- Developed and maintained multiple hybridoma colonies producing mAb 2C5

- Determined 2C5 activity using ELISA followed by 2C5 purification

TEACHING ASSISTANT, Northeastern University, Boston, MA Sep 09-

Aug 10

- Delivered lectures, solved student queries, set up exams and maintained grades

STUDENT RESEARCHER, Northeastern University, Boston, MA Jan 07 –

Aug 10

- Assisted team of Ph.D. students, postdoctoral candidates and research scientists in various projects

- Formulated sclareol-loaded mitochondria targeted liposomes and siRNA-loaded liposomes

- Routinely performed DNA isolation, DNA fragmentation assay, nuclei staining, annexin-V staining,

picogreen assay, gel electrophoresis and cell proliferation assay

- Performed routine maintenance on various laboratory instruments such as pH meter, ultra-centrifuge, flow

cytometer, fluorescence spectrometer, UV/VIS spectrometer, size analyzer, fluorescence microscope and

HPLC

PUBLICATIONS:

1. Federico Perche, Niravkumar R. Patel and Vladimir P. Torchilin. “Accumulation and toxicity of

antibody-targeted doxorubicin-loaded PEG-PE micelles in ovarian cancer cell spheroid model”. Journal of

Controlled Release, 2012, 164(1): p. 95-102.

2. Niravkumar R. Patel, Alok Rathi, Dmitriy Mongayt and Vladimir P. Torchilin. “Reversal of multidrug

resistance by co-delivery of tariquidar (XR9576) and paclitaxel using long-circulating liposomes.”

International Journal of Pharmaceutics, 2011, 416(1): p. 296-9.

3. Niravkumar R. Patel, Sophia Hatziantoniou, Aristidis Georgopoulos, Costas Demetzos, Vladimir P.

Torchilin, Volkmar Weissig, G.M. D’Souza. “Mitochondria-targeted liposomes improve the apoptotic and

cytotoxic action of sclareol.” Journal of Liposome Research, 2009, 20(3): p. 244-9.

POSTER PRESENTATIONS:

- ‘Antitumor activity of liposomal sclareol: elucidation of mechanism of apoptosis induction’, Nanomedicine

and Drug Delivery Symposium 2007, Northeastern University, Boston, MA

- ‘Reversal of Multidrug resistance by co-delivery of tariquidar (XR9576) and paclitaxel using long-

circulating liposomes’, Controlled release society 2011, National Harbor, MD

- ‘Three Dimensional Spheroid Culture: Evaluation and Comparison of Cancer Cell Behavior’. Controlled

release society 2012, Quebec City, CANADA

PROFESSIONAL MEMBERSHIPS:

American Association of Pharmaceutical Sciences (AAPS 2012), Controlled Release Society 2011 & 2012

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