Manager Assistant

ABHISHEK DASS

***, ****** ******, *** ***

Boston, MA - 02135, USA.

Email: ************.***@*****.*** Ph. No: 806-***-****

Technical Skills

Molecular Biology

RT-PCR, qRT-PCR, SDS-PAGE, Southern blotting, Western blotting, Genomic DNA

extraction, quantification of plasmid DNA and Transformation, Isoelectric focusing, Replica

platting, Alpha complementation, Restriction digestion, Designing of primers, ELISA, Molecular

Cloning, Subcloning and over expression of a gene.

Proteomics

Protein Purification, High Performance Liquid Chromatography (HPLC), Mass Spectrometry

(LC-MS/MS, GC-MS), MALDI-TOF, GelC, In-gel digestion, In-solution digestion,

Posttranslational modifications (PTM's) - Phosphoproteomics.

Bioinformatics

General: BLAST, Dot Plot, Microsoft office: Word, PowerPoint, Excel, Outlook

Proteomics: Proteome Discoverer, Global Proteome manager (GPM) and ProteoIQ.

Genomics/Next Generation Sequencing: Blast2Go, MapMan, Lasergene Suite, Tuxedo Suite, N-

Gen (DNASTAR), QSeq (DNASTAR), ArrayStar Suite.

Statistics: R Language.

Education:

Master of Science (MS) in Biotechnology Graduation: May 2013

Texas Tech University, Lubbock, TX, USA GPA: 3.75

Related Coursework

Gene Expression Analysis, Methods in Biotechnology, Biomedical Sciences Core III (Genes)

Multivariate Statistics, Analytical Separation Science, Mass Spectrometry, Introduction to High

Performance Computing, Biomedical Informatics, Scientific Communication and Bioethics.

Bachelors of Engineering (BE) in Biotechnology Graduation: May 2010

GPA: 3.8

B.M.S College of Engineering, Bangalore, India

Related Coursework

Cell and Molecular Biology, Biochemistry, Microbiology, Up-Stream processing, Down-

Stream Processing, Bioinformatics.

Research Experience:

Graduate Research Assistant January, 2012 to May,2013.

(Centre for Biotechnology and Genomics, TTU )

1. Next Generation sequencing/RNA-Seq, Illumina MiSeq

2. Shotgun Quantitative Proteomics LC-MS/MS, HPLC, GC-MS, Phosphoproteomics

3. TrueSeq, NexteraXT Library preparation of diverse samples (Peanut, Cotton, Fungus,

Bacteria)

4. Bioinformatics: R language, ArrayStar (DNASTAR), MapMan, Blast2GO, ProteoIQ,

GPM etc

5. RNA/DNA/Protein isolation from diverse samples, and quantification.

6. Preparation of media, autoclaving and maintaining inventory of necessary lab supplies.

Availability: IMMEDIATE

Research Projects:

Master's Thesis: "Molecular Development of the Mid-Stage Elongating Cotton

Fiber"

• RNA extraction, quantification, 4µg of total RNA used for Library Preparation (TruSeq Library

Prep).

• Libraries pooled, normalized (Qubit®, qRT-PCR) and 18picomolar loaded on MiSeq (Illumina

NGS). 15 million reads obtained.

• Reads assembled with N-Gen (ArrayStar) and CAP3 assemblers, and used to create a UNIQUE

FIBER TRANSCRITPOME AND PROTEOME.

• 3391 Differentially expressed transcripts identified with Q-Seq (ArrayStar). Annotation and

Putative gene functions assigned and mapped onto biological pathways (Mercator MapMan).

Shotgun Quantitative Proteomics on the Mid-Stage Elongating Cotton Fiber

• 100µg of Protein was extracted from the Cotton fiber, and was fractionated using 1-D SDS

PAGE (GelC).

• In-gel digestion was carried out and the peptides obtained were analyzed with Mass

Spectroscopy (Nano-LC-MS/MS- LTQ-XL Ion Trap Mass Spectrometer).

• The mass spectra was used to identify proteins via X!Tandem software, GPM (Global Proteome

Machine).

•384 differentially expressed proteins were identified using ProteoIQ, and annotation/functional

characterization of the differentially expressed proteins was performed using the Mercator and

MapMan software's respectively.

HPLC Method Development

• HPLC was tried as the initial fractionation step in the shotgun quantitative proteomics

mentioned above

• Optimized the HPLC method for obtaining good separation, sharp chromatography and

reducing the time of the overall run using a Peanut and Cotton protein samples.

• Compared HPLC with 1-D SDS Page. Results showed 1-D SDS Page was more suitable for

Cotton, whereas, HPLC provided efficient separation for Peanut proteins.

GelC-MS/MS for Protein Identification

• Control and test samples were Acetone precipitated, followed with 1-D SDS PAGE and in-gel

digestion on the gel lanes. Peptides obtained were analyzed on the Nano LC-MS/MS.

• 4 unique Proteins were identified using Proteome discoverer based on high confidence,

XCorr score, coverage and number of unique peptides.

Multivariate Analysis of an Out bred Stock of Fruit Flies

Discriminant factor analysis and Manova tests were performed on 2 biological data

sets to distinguish diseased population of fruit flies from normal healthy fruit flies.

“Cloning, subcloning, over expression, purification, biochemical Characterization and

functional studies of recombinant 3α-hydroxy steroid Dehydrogenase/ Carboxyl reductase”.

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Availability: IMMEDIATE

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