Maintenance State University

VAIBHAV V. JOSHI

*** ******* *****

Starkville, MS-39759

USA

Phone: 662-***-****

E-mail: *****.*******@******.***

Keywords:

DNA and RNA extraction; Assay Development; Cloning; Sequencing; PCR; q-PCR; Gene expression assays;

ELISA; Computational Biology tools; Microbiological techniques; Statistical analysis (SAS)

Work Experience:

Mississippi State University

Graduate Research Assistant

2011-2013

- Identified groundwater bacteria by PCR assay development, cloning and DNA sequencing

- Performed DNA sequence analyses using Computational Biology and Bioinformatics tools

- Accomplished gene expression analyses of pcpB and tftD genes by RNA extraction, designing

primers, cDNA library synthesis and modified RT-PCR

- Isolated and maintained cultures of bacteria; prepared various media and stock solutions

- Determined PCP concentrations in groundwater using EPA methods and Gas chromatography

- Analyzed data statistics using SAS version 9.3

- Participated actively in experimental design; maintained log books; prepared technical reports

and research articles

- Trained student workers and volunteered in laboratory maintenance

Mississippi State University

Student Worker

2011

- Performed laboratory animal (mice) maintenance; handling; sample collection (blood, peritoneal

fluid, spleen etc.); injecting compounds and RNA extraction

Education:

Mississippi State University

Master of Science, 2013

Concentration: Molecular Biology

GPA: 4.0/4.0

Maharashtra Animal and Fishery Science University

Bachelor of Veterinary Science and Animal Husbandry, 2009

GPA: 8.06/10

Skills:

Molecular Biology: Nucleic acid (DNA and RNA) extractions; purification; assay development; PCR; q-

PCR; cloning; plasmid extraction; restriction enzyme digestion; DNA sequencing; multiple sequence

alignment; database searches (NCBI, GenBank, UniProt etc.); phylogenetic analysis; Experion™ RNA

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StdSens chip analysis; designing primers; cDNA library synthesis; agarose gel electrophoresis; Southern

blotting; ELISA

Microbiology: Preparation of various growth media; sterile techniques; isolation and characterization of

microorganisms; maintenance and storage of aerobic and anaerobic microbial cultures; basic staining

techniques; BSL-2 laboratory techniques

Laboratory animal skills: Management; Handling of mice; euthanasia; injecting compounds; sample

collection: blood, peritoneal fluid, liver and spleen

Chromatography: Gas chromatography- electron capture detector using EPA method

Computational Biology software: DNA STAR, SeqMan, ClustalW, MEGA, BLAST, MUSCLE, Perl

Computer software: SAS, Microsoft Word, Excel and Power Point

Non-Technical: Team work; oral and written communication; organization and management skills;

responsibility and initiative; problem solving and ability to learn new techniques

Hands-on experience: QIAcube nucleic acid extraction, Molecular Imager® Gel Doc™ XR+ System,

Eppendorf Master Cycler, NanoDrop™ 1000 UV-Vis spectrophotometer, Experion™ RNA StdSens Chip

Analysis, Beckman-Coulter CEQ 8000 Genomic Sequencer, BIO RAD iCycler iQ® System, Agilent 6890 GC-

ECD, Autoclave

Awards:

- Awarded “Outstanding Graduate Student” award, Mississippi State University, 2013

- Secured first position in poster presentation competition at American Wood Protection

Association 109th annual meeting, Honolulu, Hawaii, USA, 2013

- Secured first position in poster presentation competition at Forest Products Society 66th

international convention, Washington DC, USA, 2012

Certification:

Completed Computational Biology certificate program at Mississippi State University

Professional Affiliation:

Honor society of Phi Kappa Phi

Publications:

Available upon request

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