Quality Control Assistant

MONICA DANALA

Email: ************@*****.********.*** Mobile: 215-***-****

OBJECTIVE:

Aspiring for a challenging position in an organization, where I can apply my knowledge and skills that

would contribute to the development of the organization and enhance my professional career.

EDUCATION:

Master’s in Pharmaceutical Sciences (Pharmacology), GPA: 3.0/4.0 Aug 2011- May 2013

Campbell University, Buies Creek, NC.

Bachelor’s in Pharmacy Aug 2007- May 2011

Jawaharlal Nehru Technological University, India.

SUMMARY OF SKILLS:

Animal research: Animal handling (mice and rat) & dosing (IV, SC, IP, IM & Oral),

surgical isolation of tissues and Lab maintenance.

Protein Biochemistry: Protein expression & purification (centrifugation & filtration),

strong academic knowledge on protein structure and composition.

Molecular Biology: Gel electrophoresis, SDS- PAGE, RNA and DNA isolation, cDNA

synthesis, End point- Polymerase chain reaction (PCR), Cell signaling, Cell based and

cytotoxicity assays, Receptor binding assays, Protein purification.

Immunology: Western blot, ELISA.

Cell Culture: In- vitro mammalian cell culture, media preparation, aseptic techniques.

Instrumentation: Confocal microscopy, High Performance Liquid Chromatography

(HPLC), UV/Vis Spectrophotometer.

Microbiology: Preparation of bacterial cultures, Gram staining.

Softwares: JMP, Graph Pad Prism, LIMELIGHT, MS office (word, excel and power point)

Writing IQ/OQ&PQ protocols, understanding of GLP & GMP regulations.

WORK EXPERIENCE:

Research Assistant at Campbell University June 2013- Aug 2013

• Trained a master’s research student in animal handling, dosing, behavioral assays and

interpretation of results.

• Involved in writing a paper for publication on master’s research project.

Research assistant at Campbell University Feb 2012- july 2012

• Six months of hands on experience in BG-1 ovarian cancer cell line maintenance,

expansion and preparation of culture media.

• Proficiency in performing cytotoxicity assays in a 96- well plate format.

• Trained in aseptic techniques, autoclaving and working in GLP based lab

environments.

• Revising/ writing SOP’s.

MONICA DANALA

Email: ************@*****.********.*** Mobile: 215-***-****

• Maintaining documentation of experimental procedures, results and submission of lab

notebooks.

• Perform statistical analysis and interpretation of results.

Intern at Shantha Biotech Pvt. Ltd, India May 2010- Jun 2010

• Studied various steps involved in vaccine production namely inactivation, purification

and formulation.

• Assisted in performing various sterilization methods involving heat sterilization,

irradiation and autoclaving.

• Trained in working in a GMP environment.

• Trained in quality control and assurance of biological products.

Miscellaneous experience:

• Six months of experience in performing DNA and RNA isolations, CDNA synthesis

and PCR on Zebra fish eggs treated with different concentrations of retinoic acid.

• One year of hands on experience in performing ELISA for the detection of viral

antigens and proteins.

RESEARCH PROJECTS:

Masters Research- Department of Pharmacology: Jun 2012- May 2013

• Worked on anti- depressant activity of Cannabinoid drugs and studied the cannabinoid

receptor signaling pathway involving ß-arrestin2 and MAPK cascades in C57Bl/6 male

mice (in vivo).

• Drugs were given IP and subjected to behavioral tests.

• Brains were isolated and hippocampal regions were examined for changes in phospho

MAPK levels by SDS PAGE followed by Western blotting.

• Protein quantification was done by Bradford assay.

Undergraduate research- Department of Pharmacology: Jan 2011- May 2011

• Worked on anti- urolithic activity of aqueous extract of Achyranthes aspera on

ethylene glycol induced kidney stones in male albino rats.

• The activity of the drug was compared against the standard furosemide drug.

• Drugs were given orally for 21 days and then sacrificed.

• Blood serum analysis, difference in kidney weights and histological microscopic

examination of kidney was performed to assess mineral content and identify the calcium

oxalate crystals.

PRESENTATIONS:

• Presented a poster on “ßarrestin-2 modulation cannabinoid activity in mice Jun

2013

varies with assay, drug and gender” at IRCS 2013, Vancouver.

MONICA DANALA

Email: ************@*****.********.*** Mobile: 215-***-****

• Presented a poster on “Anti-depressant activity of cannabinoid drugs on Apr 2013

cannabinoid 1 receptor is mediated by ßarrestin-2 and activation of

MAPK cascade” at NCAS annual meeting and Campbell University.

• Presented a paper on “Electrochemically activated microchips for Mar 2010

implanted drug delivery” at a national level seminar, India.

• Presented a poster on “Gold nanoparticles- a revolution in nanotechnology Mar 2009

for cancer treatment” at a national level conference, Hyderabad, India.

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