Steven
Ray
Road
Cell
Phone
(*06)
676-
4167
Kingston,
GA
30145
Home
Phone
(706)
295-
1701
Email:
******@***.***
Summary
of
Qualifications
Ambitious,
intelligent,
multilingual
professional
seeking
to
gain
experience
and
start
a
career
in
biomedical
research.
Innovative
and
successful
at
problem
solving,
detailed-
orientated
and
organized
with
proven
experience
in
research.
Excellent
communicator
with
great
interpersonal
skills
that
is
dependable
to
complete
the
job
and
obtain
results.
Well-
rounded
in
multifarious
techniques
in
the
lab
including:
à à
GLP/GMP Regulations Northern/Western Blotting
à à
Effective Written/Oral Communication DIC and Fluorescence Microscopy
à à
Excellent writing/documentation skills UV/VIS
à à
Microsoft Office Wet Chemistry
à à
HPLC/GC Cell Culture
à à
Solid Phase/ELISA Assays Surgical Techniques (animals)
à à
Flow Cytometry Paraffin Embedding/Sectioning
à à
Aseptic/Sterilization Techniques Histological Stains
à qRT-PCR
EDUCATION
Wake Forest University [Winston Salem, NC] December 2012
Master of Science, Molecular Medicine and Translation Science
Shorter College [Rome, GA] May 2009
Bachelor of Science, Chemistry
Bachelor of Arts, Spanish
EMPLOYMENT
Aderans Research Institute 04/2012- 08-2012
Position: Research Scientist II (short-term position worked while completing thesis)
Isolation of primary cells from human tissue
•
Responsible for mammalian cell culture of primary cells
•
Designed/wrote experimental protocols and procedures
•
Conducted laboratory experiments and data collection
•
Analyzed cells using common molecular biology techniques
•
o RT-PCR
o ELISA
o Western Blot
o Flow Cytometry
• Used/developed in vivo bioassays in mice
• Developed, designed, and implemented new bioassays for experimental procedures
(using mice)
• Conducted scientific journal and literature reviews/searches
• Analyzed data, including statistical analysis
• Prepared biweekly reports of results and planned experiments
• Collaborated with quality assurance (Q/A) and process manufacturing staff
Surgical Biologics 06/2011- 12/2011
Position: Research Associate II (short-term position worked while completing thesis)
Assisted in the development of research data, reports and study protocols
•
Conducted laboratory experiments and data collection
•
Collaborated with quality assurance (Q/A) and process manufacturing staff
•
Monitored and maintained laboratory equipment and supplies
•
Conducted scientific journal and literature reviews/searches
•
Co-developed with Director of Product Development research and development
•
policies, procedures and standards related to amnion-based products.
Interfaced with internal departments to ensure research and development plans and
•
activities are well coordinated with other key functional areas.
Assisted Director of Product Development in establishment of tactical plans to transfer
•
amnion product research concepts into a developmental environment.
Assisted Director of Product Development in meeting the demands for the
•
commercialization of new and products to support the company’s strategy for an
expanding portfolio of amnion-based products.
Converted strategic and conceptual amnion product initiatives into tactical research
•
plans to ensure the commercialization potential of all new and/or expanded products is
fully explored and developed.
Managed certain assigned projects to include creation of project plan, definition of
•
project deliverables, development of project milestones, reporting of project results
and analysis of causes for project deficiencies and delinquencies.
Assisted Director of Product Development, President of Surgical Biologics and other
•
members of Executive Management with other assigned research and development
tasks.
Wake Forest University 08/2009- 01/2011
Position: Graduate Researcher - Experience with 3 separate labs:
• Developed a solid phase assay to test different formulations of keratin hydrogels
• Designed a high-throughput solid phase assay to measure the release of cytokines
(BMP-2) from the hydrogels.
• Induced bone formations in the hind limb of mice by surgically implanting a BMP-2
saturated hydrogel
• Prepared scaffolds for use in a bioreactor by harvesting and decellularizing livers taken
from ferrets
• Cultured mouse embryonic stem cells (mES cells) along with endothelial cells to use in
bioreactor experiments
Successfully demonstrated that a decellularized organ scaffold has the capacity to
•
direct the differentiation of mES cells
Used flow cytometric assay to show that seeded scaffolds express early markers of liver
•
cells
Designed assay to show that co-cultured endothelial cells play a role in mES cell
•
differentiation
Used Northern and Western blots to measure expression of mES cell markers
•
Isolated RNA and used reverse transcription to convert RNA from mES cells to DNA for
•
use in PCR
Designed flow experiment to test differentiation capacity of recellularized scaffold after
•
underdoing directed differentiation
GC to measure levels of omega fatty acids that were used to enrich cell media.
•
Designed experiment to test the fatty acid-enriched media’s ability to affect monocyte
•
differentiation in atherosclerotic lesions
§ HPLC/GC to measure the contents of other supplements added to media
Followed GLP/GMP regulations.
•
Wrote protocols and maintained a detailed lab notebook
•
Dr. John Beninato, D.D.S., P.C. 05/2005- 03/2009
Position: Surgical Assistant
took and developed x-rays
•
responsible for patient care before and after surgery
•
monitored vital signs and communicated with patients to assess patient comfort
•
directly assisted in surgery by operating suction and assisting the surgeon directly
•
set-up rooms for procedures and stocked supplies
•
sterilized instruments and set-up trays for various procedures
•
assisted in front office filing insurance claims
•
various routine office responsibilities
•