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Quality Control Project

Location:
United States
Posted:
October 11, 2013

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Resume:

Aimee L Tripp

**** **** *** **, ***********, MN *5414

Phone: 608-***-**** : ********@***.***

Education

Bachelor of Science: Chemistry, December 2013

University of Minnesota- Twin Cities – Minneapolis, MN

Minor: Biology

Cumulative GPA: 3.62

Dean’s List: Fall 2010, Summer 2011, Fall 2012, Spring 2013

Skill Sets and Competencies

Growth, induction and inclusion body preparation of

Recombinant Protein Production and

recombinant vaccine antigens

Purification

1D SDS PAGE, IEF, 2D, and DNA Agarose gels

Electrophoresis

Protein and Carbohydrate Stains; Rotofor operation

Western Blots, ELISA assays, and Flow Cytometry

Immunology

TLC, FPLC, HPLC, affinity chromatography

Chromatography

SEC, IEX, IMAC

Reflux reactions, distillation, Rotary evaporator, and spray

Organic Chemistry Techniques

drying

Conjugation of proteins and molecules of interest including:

Combinatorial Chemistry

vaccine antigens, adjuvants, antibodies, enzymes, toxins,

fluorescent dyes, and various biotin containing reagents

Use Chitosan to synthesis Trimethyl Chitosan of varying

Polymer Modification Chemistry

“quarternization” levels and thiolated Chitosan at desirable

output levels

Building ionic hydrogels and cross-linked Nanoparticles

Hydrogels and Nanoparticles

(NPs)

Particle Size, Polydispersity Index, and Zeta Potential

Malvern Zetasizer

Measurements

1

Prepare and analyze samples with Bruker spectrometer and

H NMR Spectroscopy

Agilent Software at the University of Minnesota NMR

Center

Experience

Research Associate II

Pending, Paperwork has been submitted for promotion

Syntiron, LLC – St. Paul, MN

Create vaccine delivery systems that enhance vaccine performance by mucoadhesion, tissue penetration

properties, and/or selectively driving TH1 or TH2 responses

Synthesize Trimethyl Chitosan (TMC) with varying degrees of quarternization for analysis of tissue penetration

properties

Analyze Chitosan derivatives using 1H NMR at the University of Minnesota NMR Center. Determine degree of

quarternization and extent of chain scission and O-methylation following synthesis of TMC.

Conjugate vaccine candidates to Cholera Toxin to assess mucosal activation and production of secretory IgA

Use various Chitosan derivatives to form ionic hydrogels and nanoparticles containing vaccine candidate

antigens and adjuvants for testing in mouse and rabbit animal efficacy studies

Analyze Chitosan derivatives and nanoparticles using Malvern Zetasizer to look for change in polymer size and

surface zetapotential

Write technical monthly reports

Present current work in large group lab meetings

Techniques and Equipment: 1H NMR Spectroscopy, Malvern Zetasizer (Particle Size, PDI, and Zeta Potential),

HPLC, reversed phase and size exclusion chromatography for polymer evaluation

Research Associate I

June 2012 to Present

Syntiron, LLC – St. Paul, MN

Evaluate the following combinatorial chemistry approaches: NHS-ester chemistries using biotinylation and

fluorescent dye labeling; Thiol and Maleimide conjugation; mixed disulfide conjugation; conjugates were

purified and analyzed using desalting columns and size exclusion chromatography

Examples of products using the above approaches include: labeling IgG and IgA antibodies with fluorescent tags

for use in Flow Cytometry; producing KLH:peptide conjugates to produce epitope specific immunogens;

producing IgG:HRP conjugates for use in ELISA Assays; produce toxin conjugates using mixed disulfide linkages

An independent research project was also completed for 3 credit hours (135 hours of research) at the University

of Minnesota using the above techniques. This project resulted in high proficiency of techniques in recombinant

protein induction and purification of a His tagged Manganese transport protein, which was conjugated to

Pam3CSK4 using NHS ester conjugation approach to evaluate improvements in immune response and protective

efficacy of the conjugate.

Write technical monthly reports

Present current work to large group lab meetings

Techniques and Equipment: AKTA FPLC, HPLC, Flow Cytometry, Swiss Protein Database, BLAST sequence search

and alignment tools

Laboratory Assistant

October 2010 to June 2012

Syntiron, LLC – St. Paul, MN

General laboratory maintenance including cleaning and autoclaving glassware, ordering supplies, preparing

reagents, buffers, and growth media, and assisting the quality control group with bioburden assays to test for

vaccine sterility

Analysis of vaccine products using 1D gel electrophoresis, 2D gel electrophoresis, isoelectric focusing

Analysis of proteins using Bio-Rad Rotofor to look for separation between proteins and impurities

Analysis of vaccinate immune serum using Western Blot with iBlot transfer device and large format overnight

transfer techniques to look for the presence of antibodies to specific proteins

Awards

University of Minnesota- College of Science and Engineering Scholarship for Academic Excellence: Fall 2013

Vern Dale Scholarship for Academic Excellence: Fall 2010

Onalaska Rotary Club Scholarship: Fall 2010

Onalaska Area Business Association Scholarship: Fall 2010

Other Experience

Science Fair Judge, Twin Cities Regional Science Fair, Minneapolis, MN: Spring 2013

College of Science and Engineering Ambassador and Mentor, University of Minnesota, Minneapolis, MN:

Fall 2012 – Present

Admissions Ambassador, University of Minnesota, Minneapolis, MN: Spring 2011

Trauma and Emergency Center Volunteer, Gundersen Lutheran Hospital, La Crosse, WI: Summer 2011

Nursery Room Volunteer, First Covenant Church, Minneapolis, MN: 2012 – Present

Grounds Crew, La Crosse Country Club, La Crosse, WI: August 2006-August 2011



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