Project Manager

SHRENIK SHAH

**** ********* ***** ********* # **, Los Angeles, CA 90007

Contact no: 213-***-****; Email ID:********@***.***

EDUCATIONAL QUALIFICATIONS:

UNIVERSITY OF SOUTHERN CALIFORNIA, LOS ANGELES, CA

Keck School of Medicine

Current GPA: 3.67

Masters of Science (MS) in Biochemistry & Molecular Biology

Relevant Courses: - Biochemistry, Cell Biology, Molecular Genetics,

Protein Chemistry, Biostatistics, Structure and Management of Clinical

Trials, Industrial approaches to Drug Discovery and Design

PADMASHREE DR. D.Y.PATIL UNIVERSITY, INDIA

69.7% (First Class)

Bachelors of Technology (B.Tech)- Biotechnology

Relevant Courses: - Biochemistry, Molecular Biology, Virology,

Molecular Cell Signaling, Developmental Biology, Commercial aspects of

Biotechnology, Bioinformatics

SKILLS:

LAB SKILLS:-

Gel Electrophoresis (Agarose and SDS-PAGE)

Immunoprecipitation (Agarose and Magnetic)

Western blotting

Microbial batch culturing and IPTG induction

Protein Extraction and purification by microfluidization,

lyophilization and dialysis

HPLC and Reverse Phase HPLC based ultrafiltration

Protein analysis by spectrophotometry

Radioactive (I131) and Fluorescent (FITC) labeling of Proteins

FACS based cell analysis and cell sorting

Immunoflourescence

Maintenance of Mammalian Cell lines (Primary. Transformed and cancer

cell lines), Plant tissue culture

Cell migratory assays (Colloidal gold assay, scratch/ wound healing

assay)

Light microscopy, Confocal Microscopy

Autoclaving of media and sterile reagent preparation

Chromatography

DNA Isolation

PCR, qRT-PCR, ELISA, Cell counting, Blood typing, Coagulation assay

COMPUTER SKILLS:-

SPSS, Graph-pad, Rasmol, Swiss pdb, Modeller, Chemsketch, Image Studio,

Marvin sketch, Argus Lab, Bioedit, Ligand Scout, DS Visualizer,

Biological databases, MS Office, MS excel.

RESEARCH EXPERIENCE:-

Research Associate under Dr.Francis Markland at USC

Project title: Effect of Vicrostatin on Integrin based signaling

molecules in Cancer. Jan 2012-Jun2013

My research project involved analysis of the effect of recombinant

disintegrin molecule Vicrostatin (VCN), on integrin based intracellular

signaling in cancer cells. The aim of the project was to study the anti-

migratory effect of VCN, from cell movement till the effect on

intracellular binding partners involved in cell movement and migration.

I independently carried out production of VCN using bacterial

expression system and further purified VCN by lyophilization, dialysis

and Reverse Phase HPLC.I studied the inhibitory effect of VCN on cell

migration using cell migration assays. Using western blotting and

Immunoprecipitation, I was able to elucidate the inhibitory effect of

VCN on interactions of three of the integrin binding partner's i.e

Talin, Vinculin and Actin.

Project title: Integrin profiling of Canine Osteosarcoma cell lines

October 2012-Present

The primary phase of this project involves profiling and determination

of various disintegrin targets present on 5 different Canine

Osteosarcoma cell lines by immunofluorescence and confocal microscopy

and further confirming it by FACS analysis. The next phase

involves assessing the anti-metastatic effect of Vicrostatin on the

osteosarcoma cell lines and their comparative analysis.

Intern at Dr. D.Y. Patil University, Mumbai, India

Project title: Extraction of Sulforaphane & assessing its Anti-tumor

and Anti-microbial Activity. Jan-11-May-11

The first stage of this project involved isolation of a potential anti-

cancer compound, Sulforaphane from broccoli and assessing its anti-

tumor activity on HeLa (cervical cancer) and MCF-7(breast cancer) cell

lines, The next stage involved assessing its anti-bacterial activity on

highly infectious gram negative and gram positive bacteria. The later

stage included combinatorial study of Sulforaphane with Allicin

(compound from garlic) and Eugenol (compound from Indian medicinal herb

Tulsi i.e Ocimum sanctum )to see its combinatorial effects

SEMINAR AND OTHER ACHIEVEMENTS:

Currently enrolled as distance student with WIPO for Patent management

studies.

Completed NIH certification course on Good Clinical Practices.

Mentor of USC undergraduate and high school students under the USC-

STAR Program.

Completed Certification Course in Clinical & Translational Science

conducted by SC CTSI, USA.

Student Store Manager at USC bookstores (Catalog Department).

Attended National Seminar and Technical festival on "Frontiers in

Biotechnology."

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