Development Medical
Resume:
ALEXEI ALEXEEV
**** ********** ***. #**, ***** Clara, CA 95051
e-mail: *************@*******.***
OBJECTIVE A challenging research position in Pharmaceutical Industry.
SUMMARY OF QUALIFICATIONS
Pharmaceutical research and development professional with extensive experience in Biochemistry, Immunology
and Microbiology including development of health care products encompassing Drug Delivery Systems,
Vaccines, Diagnostics and Biologicals. Strong blend of scientific and business skills with an in-depth knowledge
of the drug development process, including scientific, development, clinical and regulatory requirements.
TECHNICAL SKILLS
* Biochemistry: protein purification; gel electrophoresis of proteins and nucleic acids and blotting;
ion-exchange chromatography; enzyme assays; experience with radiolabeled compounds;
spectroscopy; HPLC, maintenance of mammalian cell cultures: breast and prostate cancer cells,
human neonatal dermal fibroblasts and melanoma cells, bovine endothelial capillary cells.
* Pharmacology: experience with polymeric formulations including biodegradable microspheres, implants
and oral delivery systems, in vivo chronic and acute pharmacology/ toxicology with mice and rabbits.
* Immunology: ELISA, RIA, polyclonal antibody production, affinity chromatography, DTH,
Plaque-forming assay, MLR, T cell proliferation assay, mammalian cell culture.
* Microbiology: whole-cell and acellular vaccine formulation and development, bacterial protein
purification, characterization and inactivation, experimental infection in laboratory animals.
PROFESSIONAL EXPERIENCE
2012- June 2013 Kateeva Inc. Menlo Park CA Sr. Chemist
2010 – March 2011 Biophor Inc./ Biostride Inc. Redwood City, CA Scientist
- Running Hamilton MicroLab Starlet system (RapidFRET) for automated drugs of abuse testing.
- Purification mAb against variety of hormones and drugs of abuse.
2002 – 2008 Sunny Biodiscovery, Inc. Santa Clara, CA Sr. Scientist
- Study the efficacy of different compounds from botanic sources that possess anti-inflammatory,
anti-cancer, angiogenic/anti-angiogenic, immunomodulating and wound healing features.
Major job responsibilities:
- Isolation, purification, characterization of polysaccharides, peptidoglycans, proteins and peptides from
Lycium barbarum L., and both in vivo and in vitro evaluation of their biological activity.
- Drug screening, biological target evaluation, establishing the mechanism of action for compounds of interest.
- Formulation of slow release solid and semi-solid drug delivery devices (rods, tablets, pouches on the basis of
PLA, PLGA, DEAE- or CM-cellulose).
- Maintenance of mammalian cell cultures.
- Investigation of novel anti-microbials against aerobic/anaerobic bacteria.
- Cell Based Assay services (cell proliferation and cytoxicity, cell migration/adhesion, kinase activity, lipid
peroxidation, anti-oxidant activity, re-endothelialization, multiplex cytokine assays).
1998 - 2002 Oculex Pharmaceuticals, Inc. Sunnyvale, CA
Sr. Microbiologist/Analytical Scientist
- Provided pre-clinical support for the drug research program to prove feasibility of novel anti-microbial
and anti-inflammatory drug delivery systems. Planned and conducted microbiological evaluation study of
new compounds; time-killing kinetics, antibiotic resistance; AUC, MIC, MBC and IQ investigation.
- Performed an in vitro and in vivo release testing of drug delivery devices using Reverse Phase HPLC
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- Development of Biodegradable Drug Delivery Systems (rods, tablets, gels) and topical drops for the
treatment of ophthalmic diseases.
- Selected, characterized and evaluated polymers and drugs for ophthalmic drug delivery systems.
- Performed optimization of release testing study (protein concentration, pH, temperature) to
achieve a close correlation of in vitro/in vivo data.
- Developed and validated an accelerated methods for drug release, stability and polymer degradation analysis.
- Developed and validated HPLC method for analysis of various drugs in plasma in supporting PK studies.
- Studied the influence of various environmental conditions on an in vitro drug release.
1987-1996 GAMALEYA INSTITUTE OF EPIDEMIOLOGY AND MICROBIOLOGY,
Russian Academy of Medical Science, Moscow, Russia
Lab. of Acute Meningitis and Lab. of Pertussis and Other Bordetellas,
Senior Researcher
- Formulation development of Whole-cell and Acellular Oral Vaccine against Pertussis
- Development of new methods of pertussis toxins inactivation.
- Experimental studies of effectiveness of vaccines against pertussis and meningococcal infections.
- Synthetic non-natural biodegradable polymers as a novel class of immunomodulators.
- Phenotypic correction of immune response.
The routine work included planning, designing and conducting experiments using:
- Test vaccines preparation for pre-clinical/clinical studies.
- Radioactive labeling of bacterial cells and bacterial antigens.
- Purification of bacterial, viral and fungal antigens.
- Performing safety and stability studies according cGMP requirements.
- SDS-PAGE and Western blotting.
- Preparation of liposomes, ISCOMs, polyclonal antibodies and antibody purification.
1977-1987 USSR CARDIOLOGY CENTER, Moscow, Russia
Laboratiry of Immunology,
Senior Researcher
Conducted the following research studies:
- Biochemical and immunological studies on atherosclerosis, hypertension and other vascular diseases
- Target transport of immunoliposomes to the vessel walls’ injury sites;
- Evaluation of cardiovascular therapeutic compounds in in-vitro and in vivo models
- Chronic inflammatory diseases in laboratory animals (NZB/NZW mice and other models) and humans
(RA, Juvenile RA, SLE, Sclerodermia, Sjogren' Syndrome)
EDUCATION
M.D., Moscow State Medical University, Russia
Ph.D. in Immunology and Microbiology, Gamaleya Institute of Epidemiology and
Microbiology, Academy of Medical Sciences of the USSR, Moscow
ADDITIONAL TRAINING
Short course in method development and validation offered by Waters
HPLC class at Foothill college
PERSONAL U.S. citizen
REFERENCES Available upon request.
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