Anuj Mankad, Ph.D.

*** *. ******** ***., **********, NJ 07095

Phone: 908-***-**** (cell)

E-mail: ab8qyx@r.postjobfree.com

http://www.linkedin.com/pub/anuj-mankad/8/918/3a4

A meticulous individual with a helpful and collaborative nature seeks

to contribute his technical, analytical, critical-thinking, and scientific

communication skills to biomedical research.

Education:

2006: Ph.D., "Mutation analysis in twins with Fanconi anemia and

modifications to a gene mapping technique" Department of Molecular and

Medical Genetics, Oregon Health & Science University, Portland, OR.

1998: B.Sc., Biology; B.A., Computer Science, Brown University, Providence,RI

Undergraduate and graduate coursework covered general and molecular

genetics, eukaryotic genetics and cell biology, biochemistry, molecular

mechanisms of disease, developmental biology, evolutionary biology,

computational molecular biology, and basic neuroscience.

Employment History:

2013 (current): Contractor with WyzAnt Tutoring Services

Teach concepts and theories of general and molecular genetics and

molecular biology

http://www.wyzant.com/Tutors/NJ/Woodbridge/8077314/

(mid-2011 to present: searching for a full-time position in biomedical

research and/or education)

2009-2011: Postdoctoral Research Scholar / Research Investigator,

Laboratory of Robert Cornell, Ph.D., Department of Anatomy and Cell

Biology,Carver College of Medicine, University of Iowa, Iowa City, IA

Utilized the zebrafish model to study the phosphorylation of Irf6, a

transcription factor involved in palate formation and clefting, as

well as in wound healing and breast cancer.

2006-2008: Postdoctoral Research Fellow,

Laboratory of Qishen Pang, Ph.D., Division of Experimental

Hematology,Cincinnati Children's Hospital Medical Center, Cincinnati, OH

Investigated the ability of patient-derived acquired mutations to

FANCA, one of the genes involved in Fanconi anemia, to compensate for

inherited mutations in this same gene. Fanconi anemia is characterized

by bone marrow failure and a predisposition to acute myeloid leukemia,

head and neck squamous cell carcinomas and other solid tumors.

1997 (summer): Research Intern, Computer Modeling Group, Biogen Inc.(Biogen-IDEC)

Assessed utility of various programs for analyzing Protein Data Bank

(PDB) files, and ran sequence database searches for genes and

proteins.

Grants awarded:

2006-2008: Grant from the Fanconi Anemia Research Fund for independently

conceived and designed project, "Characterization of the FANCA Protein and Patient-

Derived FANCA Mutants", partially funded first post-doctoral research fellowship.

Transferable Laboratory Skills:

. plasmid construction (digestion & ligation, TA-cloning, Gateway, etc.)

. bacterial propagation of plasmids

. PCR and site-directed mutagenesis

. gel electrophoresis and Southern blot

. SDS-PAGE & Western blot

. fluorescence microscopy

. immunofluorescence & immunohistochemistry

. immunoprecipitation

. subcellular fractionation

. RNA and DNA extraction from cells

. general tissue culture techniques, including culture of primary fibroblasts, cell lines, and immortalized lymphoblasts

. electroporation and lipofection

. retroviral infection (and titer)

. 96-well cell proliferation / death assays

. fluorescence & luminescence reporter assays

. zebrafish embryo injection (DNA, RNA, morpholinos)

. deyolking of early stage zebrafish embryos

. in situ hybridization

. cell synchronization

. single-color flow cytometry

. mouse colony maintenance & genotyping

. derivation of cells from mouse tail slice

. microcell mediated chromosome transfer

. paraffin section DNA extraction

. writing protocols

. use of bioinformatics sites (ClustalW, BLAST, PubMed, Ensembl, etc.)

. proficiency with Microsoft Word, Excel, Access, and PowerPoint, as well

as Adobe Photoshop, MacVector and Prism

Teaching experience:

. Supervised visiting researchers, and instructed them in molecular cloning

techniques, during both post-doctoral fellowships.

. Taught classes in Population Genetics, Immunogenetics, Developmental

Genetics, and Hemoglobinopathies in a faculty-supervised course in

graduate school.

. Educated other college students on the various programming models used to

store and organize data as a Teaching Assistant for Algorithms and Data

Structures, a computer science course, at Brown University.

Publications:

. de la Garza G, Schleiffarth JR, Dunnwald M, Mankad A, Weirather JL, Bonde

G, Butcher S, Mansour TA, Kousa YA, Fukazawa CF, Houston DW, Manak JR,

Schutte BC, Wagner DS, Cornell RA. Interferon Regulatory Factor 6

Promotes Differentiation of the Periderm by Activating Expression of

Grainyhead-Like 3. J Invest Dermatol. 2013 Jan;133(1):68-77. doi:

10.1038/jid.2012.269.

. Mankad A, Taniguchi T, Cox B, Akkari Y, Rathbun RK, Lucas L, Bagby G,

Olson S, D'Andrea A, Grompe M. Natural gene therapy in monozygotic twins

with Fanconi anemia. Blood, 2006 Apr 15;107(8):3084-90.

. Cosma MP, Pepe S, Parenti G, Settembre C, Annunziata I, Wade-Martins R,

Di Domenico C, Di Natale P, Mankad A, Cox B, Uziel G, Mancini GM,

Zammarchi E, Donati MA, Kleijer WJ, Filocamo M, Carrozzo R, Carella M,

Ballabio A. Molecular and functional analysis of SUMF1 mutations in

multiple sulfatase deficiency. Hum Mutat. 2004 Jun;23(6):576-81.

. O'Connor M, Lee WM, Mankad A, Squires CL, Dahlberg AE. Mutagenesis of the

peptidyltransferase center of 23S rRNA: the invariant U2449 is

dispensable. Nucleic Acids Res. 2001 Feb 1; 29(3):710-5.

Presentations:

. Mankad A, Pang Q. Functional correction of two inherited FANCA mutations

via substitution of Glu966 with lysine or alanine. 20th Annual Scientific

Symposium, Eugene, OR, October 4-7, 2008

. Mankad A, Pang Q. Characterization of the effect of FANCA mutations on

biochemical and cellular functionality. 19th Annual Scientific Symposium,

Chicago, IL, October 8-11, 2007

. Mankad A, Taniguchi T, Cox B, Akkari Y, Olson S, D'Andrea A, Grompe M.

Natural "Embryonic Gene Therapy": Acquired Secondary Mutation in

Monozygotic Twin Sisters with Mutation in FANCA Gives Rise to Corrected

Hematopoietic Lineage of Both Sisters. 15th Annual Fanconi Anemia

Research Fund Scientific Symposium, Houston, TX, October 16-19, 2003.

. Mankad AN, Cosma MP, Cox BA, Reifsteck C, Olson S, Grompe M. Use of

microcell mediated mouse chromosome transfer for gene localization. 51st

annual meeting of the American Society of Human Genetics, San Diego, CA,

October 12-16, 2001

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