Project Management

LORRAINE SUHADOLNIK

**** ********** ****, ***** ***** PA 19038 215-***-**** (H); 215-***-**** (C)

******************@*****.*** www.linkedin.com/in/lorrainesuhadolnik

Professional Summary

Experienced pharmaceutical professional with more than 15 years of experience in medical research.

Experienced at independently conducting and supporting full range of scientific research with proven

proficiencies in project management, research compliance of standard operating procedures, data

assessment, documentation and reporting. Experience includes supervisory training and oversight of

new personnel. Skilled in oral and written communication at various organizational levels and

accomplished contributor in cross-functional work environments.

PROFESSIONAL EXPERIENCE

Lancaster Laboratories, Lancaster, PA 2010-2012

Scientist, Professional Staffing Group

Performed cGMP testing of samples for biopharmaceutical clients employing various bioassay methods. Carried

out method transfers, feasibility testing and method development following strict SOP requirements. Initiated and

performed investigations and execution of assay validation requirements. Communicated effectively with client

personnel on all documented results as required by company policy.

• Scheduled and implemented bioassay experiments for study samples as submitted by project leads

• Analyzed, documented and reported results to project coordinators

• Mentored, trained and supervised the orientation of fifteen new hire scientist

Teacher’s Assistant 2009-2010

Tutored and mentored sixth through twelfth grade students in science and math. Provide personalized instruction

for ACT, PSAT and SAT preparation. Prepared, advised and edited resumes for summer interns, college graduates

and professionals seeking career opportunities.

WYETH RESEARCH, Collegeville, PA 1995- 2008

Research Scientist II, Women's Health and Musculoskeletal Biology (2000-2008)

Developed and executed in vitro functional bioassays for fast track projects under specific and critical timelines.

Emphasis on advancing projects, either independently or as part of a team. Developed and validated new assays

related to receptor function and the effects of hormone treatments. Introduced and validated yeast-based assays,

mammalian 2- hybrid systems as well as the gene expression by baculovirus and adenovirus systems.

Developed biochemical bioassays to monitor neuronal transmission in the brain specifically as related to

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enzymatic reactions effecting female sexual dysfunction. Generated, analyzed and communicated results

to team members. Project status was advanced on development track months before the scheduled

completion date.

• Supervised three scientists in development of secondary back-up assays for project support. Reviewed

and analyzed all data and progress of Cell-based assays as they were developed for project support.

Supervised junior personnel members on molecular biology techniques, assay development, efficient

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execution of experiments and analytical review of data. Directed and mentored junior scientist on the

intricacies of various laboratory procedures including basic laboratory techniques, method development,

assay validation and documentation. Trained scientists on analytical programs for data interpretation.

Research Scientist I, Women’s Health Research Institute (1997-2000)

Lorraine Suhadolnik Page 1

Conducted research on nuclear receptors supporting the evaluation of potential targets by analyzing molecular

mechanisms involved in tissue-specific regulation. Characterization of targets and compounds obtained from HTS

were determined by ELISA, gel shift, Western and cell-based transfection assays.

Developed primary assay for meiotic gene expression as related to male fertility. Focus

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on development of a non-hormonal drug that would disrupt the meiotic DNA break repair of the MSH5 gene

for the purpose of male contraception. Assisted in the protein sequence design for antibody production.

Isolated, purified and tested the MSH antibodies for reactivity.

Performed qPCR analysis of gene regulation in phases of meiosis. Used tissue specific

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mRNA obtained from mice at different stages of development. Analyzed data representing the progression of

genes as they were up-regulated and down-regulated in different meiotic phases.

Provided support for yeast, insect and mammalian cell-based assays including

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subsequent analysis of data and submission of data to company database and documentation of all protocols.

Senior Molecular Biologist, Women’s Health Research Institute (1995-1997)

Supported the research of three senior scientists: maintained and supplied necessary tissue culture, performed

transient transfections and treated appropriately, generated baculovirus as needed, and analyzed and presented

data with future experimental focus.

Characterized cell lines with regard to estrogen receptor in order to identify and profile candidate

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compounds.

Analyzed the estrogenic response of candidate “hot flush” genes by developing second-

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generation gene transcription assays to profile candidate compounds. Genes were analyzed using molecular

biology techniques and results presented to the group along with follow-up experimental plans.

Technical Expertise

SOP Compliance: Accountable for recording progress in notebooks, presenting data at meetings, coordinating lab

responsibilities, literature searching for new assays and information to aid in the advancement of projects.

Project Management: scheduling work assignments, time management, project management including

establishment of timelines and deliverables.

Corporate Citizen & Communications: Training & Supervision of new personnel, Manage laboratory

compliance procedures & maintenance, Task force member for company & departmental activities events,

PowerPoint Presentations at inter and intra-departmental meetings, Poster Presentations at scientific meetings

Assay Development: Optimization, Validation & Standardization of assays, miniaturization of assays for high-

medium throughput screening; types: GPCRs, Enzyme, Transfections, Cell-Based, FRET

Screening: Low to medium throughput screening, Chemistry liaison, Data analysis & documentation

Molecular Biology: all techniques utilized in this area of research

Protein Expression: Protein production and purification

Tissue Culture

Computer Skills: Microsoft Office: Excel, PowerPoint, Word, Statistics Data Bases, Graph Pad Prism, Sequence

Applications (Alignments; Blast; Translational)

Education

BA, Chemistry, Temple University, School of Arts and Sciences, Philadelphia, PA

Development Training: Compliance, Ethics, Data/Documentation, cGMP/SOP

Lorraine Suhadolnik Page 2

Technical Addendum

Publications

Panayiotis Stevis, Darlene C. Deecher, Lorraine Suhadolnik. Larry M. Mallis and Donald Frail, "Differential

Effects of Estradiol and Estradiol-BSA Conjugates'" Endocrinology 140. 5455-5458 (1999)

K. Kariko, R. W .Sobol, Jr., L. Suhadolnik. N.L.Reichenbach, R.J. Suhadolnik, I and W. Pfleiderer,

"Phosphorothioate Analogues of 2', 5'-Oligoadenylate. Enzymattically Synthesized 2',5'-Phosphorothioate Dimer

and Trimer: Unequivocal Structural Assignment and Activation of RNase L", Biochemistrv 26 7127-7135 (1987).

K. Karilo, S.W. Li, R.W. Sobol, Jr., L. Suhadolnik. N.L.Reichenbach, R.J. Suhadolnik, R. Charubala and W.

Pfleiderer, "Phosphorothioate Analogues of 2',5'A: Elucidation of the Stereochemical Course of the Enzymes of

the 2-5A Synthetase/RNAse L System", Nucleosides & Nucleotides 6, 173-184(1987).

Abstracts

Alfinito P.D., Suhadolnik L., Cosmi S., Chen X., Stephens C., Pawlyk A., Winneker R., Deecher D., Neuronal

Nitric Oxide Synthase is a Novel Target for the Development of Nonhormonal Treatments for Vasomotor

Symptoms Associated with Menopause [Abstract] WHMSB Retreat 2007.

Chengalvala M, Suhadolnik L., Shanno L., Kopf G. Interaction of the Androgen Receptor and Nuclear Receptor

Corepressor as a Target for Developing Selective Androgen Receptor Modulators. [Abstract] Wyeth

Discovery Retreat 2006.

Shen E, Bapat A, Chen G, Chengalvala M, Chennathukuzhi V, Hoffman W, Kopf GS, Shanno L, Stevis P,

Suhadolnik L, Oocyte-specific BMP Family Members, GDF9 and BMP15: Potential Targets for Female

Contraception. [Abstract] Discovery Retreat 2005.

Samuel M, Lane M, Suhadolnik L, Merchenthaler I, Kopf G, Stevis P. Calbindin-d9k expression is regulated by

estrogen in the hypothalamus of the rat: close association with estrogen receptor-alpha (ERalpha). In vivo

confirmation of microarray data utilizing an immortalized cell line (D12). [Abstract] Endocrine Society Annual

Meeting, Boston, MA, Jun 24, 2006.

Suhadolnik L, Stevis PE. Activation of multiple signaling pathways by estrogen receptor alpha and beta in the

presence of antagonist. [Poster Session] 86th Annual Meeting of the Endocrine Society, New Orleans, LA, Jun

12-18, 2004. UI: 7749.

Zhang Z, Lundeen SG, Zhu V, Carver JM, Suhadolnik L. Stevis P.,Winneker RC,"ln-Vitro Characterization of

trimegestone: A New Potent and Selective Progestin"Progesterone, Progestines And Antiprogestines, Jerusalem

(1999).

H. R. Hubbell, K, Kariko, R.W. Sobol, Jr., S. Pombanlualap, S.W. Li, L. Suhadolnik. N.L. Reichenbach D.H.

Gillespie, W.A. Carter, I. Brodsky, p. Sheets and R.J. Suhadolnik, "Human Heterogeneous Nuclear RNA-

Activated 2-5A Synthetase Produces Biologically Active 2-5A", Fed. Proc. 46. 2014 (abstract 515) (1987).

K. Kariko, R.W.Sobol, Jr., L. Suhadolnik. N.L.Reichenbach, R.J. Suhadolnik, R. Charubala and W. Pfleiderer,

"Phosphorothioate Analogs of 2-5A: Activation of RNase L by Trimer Cores and 5'-Monophosphates'" Fed. Proc.

46. 2083 (abstract 922) (1987).

Lorraine Suhadolnik Page 3

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