Sandeep Krishna Ramineni

*** ****** ******, *** * Email: ab89d0@r.postjobfree.com

Lexington, KY-40508 Mobile: 859-***-****

SUMMARY

• Five years of experience in tissue-implant interface lab working on wound healing projects like

oral dysplasia, oral tissue regeneration and oral mucositis

• Proven experience in product development from concept to critical animal studies, planning to

achieve goals in time, selection of various characterizing techniques/experiments for successful

medical device

• Design and development of biodegradable polymer based controlled release drug delivery

systems

• Experience with mucoadhesive polymers, surgical meshes and their coatings

• Demonstrated experience in delivery of proteins and several small molecules

• Bioactivity assays and cell material interactions using mammalian cell cultures

• Adept experience in mechanical testing of soft tissues, films and Dynamic Mechanical analysis of

complex materials

• Perform in vivo studies, anesthesia, injections, euthanization, implant retrieval and assist in

surgeries.

• Overall proficient knowledge in field of biomaterials, biocompatibility and tissue regeneration

• Experience in programming language, web designing and development of bioinformatics tool

EDUCATION

• PhD in Biomedical Engineering (Aug’08 to

present)

University of Kentucky, Lexington, KY GPA: 3.83/4.0

Dissertation: Successfully developed and characterized a complete medical device (biodegradable

controlled release films) for treatment of various oral disorders

o Important studies such as tuning of release profiles, tensile, adhesion, transport kinetics,

permeability characteristics, cell-material interactions and bioactivity were thoroughly conducted

o Transitioned to in vivo studies to achieve ultimate goal of treatment

• Bachelor of Technology in Biotechnology (Aug‘04-

Aug’08)

Vellore Institute of Technology, Vellore, India GPA: 8.35/10

Internship: Shanta Biotechnics Limited, India, (Apr’06- Jun’06)

o Gained working knowledge in fermentation process of proprietary bacteria

o Cloning and hybridization of plasmids techniques for production of Hepatitis-B vaccine were

practiced

o Gained valuable experience by working in GMP standard quality control center on different

analytical techniques involved in quality control procedures of recombinant protein.

TECHNICAL SKILLS and HANDS ON EXPERIENCE

• Tensile, compression and dynamic mechanical testing (DMA), pull-off and shear adhesion testing

of materials, tissues and combination of both (Bose and Instron)

• Characterization of polymers using Differential scanning calorimetry(DSC) and

Thermogravimetric analysis(TGA), Dynamic Light Scattering(DLS)

• Imaging techniques like Scanning electron microscopy(SEM), phase contrast microscopy and

whole animal imaging using IVIS system

• Paraffin and cryo-sectioning of tissues; H & E staining

• Cytotoxicity and proliferation assays of mammalian cell cultures

• Tagging of proteins and small molecules with dyes and radiolabeling

• Protein based assays like ELISA, western blotting and quantification techniques

• Separation, purification and quantification of molecules by Reverse Phase HPLC, GPC

• Adept experience on programming language ‘C’ and web language ‘PHP’

• Extraction and quantification of DNA, RNA from mammalian and plant cells

• Working experience on PCR and RT-PCR, SDS-PAGE

• Basics of genetic engineering techniques and Bioprocess engineering

• Experience in writing proposals, IRB and IACUC protocols

ADDITIONAL RESEARCH EXPERIENCE

• Research Assistant, University of Kentucky, KY (Dec 2011-Till

date)

-Experience in delivery of proteins by developing films for accelerated wound healing of mucosal

tissues

-Achieved vast cell culture experience by determining bioactivity, cytotoxicity and efficacy of system

in vitro on multilayered 3D oral mucosa epithelial rafts by creating wounds of 3mm diameter

• Research project, University of Kentucky, KY (Jan 2012-Till

date)

-Broadened my knowledge on other disorders by studying impact of Doxycycline on Hernia repair

-Improved my experience in handling of animals, extraction and storage of tissues (repaired hernia

defect areas along with proprietary meshes)

-Tensile testing of complex materials (soft tissues with meshes) sharpened my mechanical testing skills

• Research project, University of Kentucky, KY (Mar’12- Till

date)

-Dynamic mechanical analysis of proprietary material to find its natural frequency

-Evaluate the design of material for its potential to absorb energy during high impact forces

-Transmissibility of the material was evaluated for different designs and compared

• Research project, University of Kentucky, KY (Jan’09-

May’09)

-Diverged skill set by constructing Continue Wave Near-infrared Tissue-oximeter based on a circuit

-Refreshed coding skills by developing user interface program for controlling machine using

“LABVIEW” software and measure oxygenation of tissue at various locations

INDUSTRIAL RESEARCH EXPERIENCE:

• Research Intern, International Institute of Information Technology (IIIT), India (Dec’07-

Jun’08)

-Proven ability of using programming languages and web development by developing dynamic web

based tool to determine ‘Genomic islands’ - “Integrated Genomic Island Prediction Tool (IGIPT)”

-Complex program to analyze and predict genomic islands from ‘Megabytes’ of genome data.

-This work was published and widely used by various groups at this point of time

• Student Intern, Prabhat Agri Biotech Limited, India (May’07-

Jun’07)

-Exposure to basic screening techniques of transgenic cotton species to further improve yields

-Preparation of transgenic species of cotton using plasmids

PUBLICATIONS AND PRESENTATIONS:

• Ramineni S.K., Cunningham L.L.Jr., Dzuibla T.D., Puleo D.A., Development of imiquimod

loaded mucoadhesive films for oral dysplasia, J Pharm Sci, 2013, 102 (2), 593-603.

• Ramineni S.K., Cunningham L.L.Jr., Dzuibla T.D., Puleo D.A., Competing properties of

mucoadhesive films designed for localized elivery of imiquimod, Biomaterials science, DOI:

10.1039/C3BM60064E, 2013.

• Tharappel J.C., Ramineni S.K., Puleo D.A., Roth J.S., Doxycyline impacts hernia repair

outcomes, submitted to Journal of Surgical Research, 2013.

• Ruchi J, Sandeep R, Nita P, “IGIPT-Integrated Genomic Island Prediction Tool (IGIPT)”,

Bioinformation, 7 (6), 307, 2011 (Publication).

• Vijesh K B, Sandeep R, “Photodynamic Therapy”, National Conference Ichescon’06, Warangal,

India, 2006 (Oral presentation)

• Multiple poster and oral presentations at conferences

ACHIEVEMENTS:

• Recipient of ‘International scholarship award’ at University of Kentucky, 2009

• Awarded ‘Kentucky graduate scholarship’ for good academic performance at University of

Kentucky, 2008-2013

• Got admitted in to one of the Top 20 schools of India in to Biotechnology program of Vellore

Institute of Technology

PROFESSIONAL AFFLIATIONS:

• Society for Biomaterials (SFB) 2008-

Present

• University of Kentucky student chapter of Society for Biomaterials (SFB) 2010-

Present

• American association for advancement of science (AAAS) 2010-

Present

• American association for dental research (AADR) 2011-

Present

ACTIVITIES:

• Secretory of Indian Student Association (ISA), University of Kentucky 2009-

2011

• Organizer of National conference in Biotechnology at Vellore Institute of Technology Dec-

2007

• Self-owned non-profit group to support education of poor and needy children 2010-

Present

and provide loans for small sustainable business

• Association for India’s Development (AID), Volunteer 2007-

Present

RELATED COURSE WORK:

Biomaterials science and engineering, Tissue Implant Interface, Drug delivery, Nanotechnology,

Biostatistics, Mechanical properties of materials, Biosolid mechanics, Musculoskeletal mechanics,

Biomedical Instrumentation, Bioinformatics, Mass transport and separation, Thermodynamics, Molecular

biology, Biochemistry, Cell biology, Protein engineering, Genetic engineering, Genetics, Animal

physiology.

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