Engineering University

Contact

Melissa Kotterman telephone: 412-***-****

**** ****** ******, ********* *, Berkeley, CA 94709 e-mail:

*******.*.******@*****.***

Education

**** ** ******* **.*. in Chemical and Biomolecular Engineering

University of California at Berkeley, Berkeley, CA

Overall GPA: 3.8/4.0 Expected Graduation: May 2013

National Science Foundation Graduate Fellowship, awarded 2008

Thesis Title: Directed Evolution of Novel Adeno-Associated Viruses for Therapeutic

Gene Delivery

2004 to 2008 B.S. in Chemical Engineering and Biomedical Engineering (dual major)

Carnegie Mellon University, Pittsburgh, PA

Overall GPA: 3.71/4.0 Major GPA: 3.89/ 4.0

Graduated with University Honors

Carnegie Institute of Technology Dean’s List: Spring 2006, Fall 2006, Spring 2007, Fall

2007, Spring 2008

Relevant Skills

Biology Techniques rodent intracranial stereotaxic injections; rodent sacrifice and tissue harvest; mammalian/stem cell

and Assays tissue culture; high throughput cell imaging; flow cytometry; immunocytochemistry; adenovirus,

adeno-associated virus, and lentivirus production; molecular biology techniques including cloning,

PCR, and quantitative PCR

Software MS Office (Excel, Word, PowerPoint), Photoshop

Academic Experience

University of California, Berkeley

Graduate Student Researcher

Advisor: Dr. David Schaffer August, 2008 to present

- Developed and studied novel viral variant capable of significantly better gene delivery and gene targeting

of human pluripotent stem cells using directed evolution

- Utilized viral gene delivery to significantly increase specificity and efficiency of gene delivery to

mammalian neural stem cells in vitro and in vivo

- Developed and studied novel viral variants capable of 35-fold better evasion of anti-virus neutralizing

antibodies in vitro and in vivo using directed evolution

University of California, Berkeley

Graduate Student Instructor

Biochemical Engineering August, 2011 to December, 2011

- Assisted in the teaching of a 45 student course on the use of chemical engineering skills and principles

in the analysis and design of biologically-based processes

- Lead one-hour discussion sections twice weekly to reinforce lecture material

- Designed homework assignments and assisted in designing exams to evaluate student performance

University of California, Berkeley

Graduate Student Instructor

Chemical Engineering Laboratory August, 2009 to December, 2009

- Lead four-hour lab sections twice weekly in which students performed 18 small-scale experiments to

reinforce chemical engineering design principles

- Evaluated student performance through oral presentations, weekly pre-lab assignments, and a final

written exam

- Facilitated the transition of the course from a two semester course to a one semester course

Carnegie Mellon University

Undergraduate Student Researcher

Advisor: Dr. Nisha Shukla September, 2006 to August, 2008

- Developed novel synthesis methods for platinum, iron-platinum, nickel, and cobalt nanoparticles

- Characterized metallic nanoparticles

- Developed novel coating method for the creation of gold nanoparticles with chiral surfaces

- Characterized the ability of chiral gold nanoparticles to separate enantiomers of various chemical

compounds

- CMU Small Undergraduate Grant award winner, Spring 2007, Fall 2007, Spring 2008

Carnegie Mellon University

Undergraduate Student Researcher

Advisor: Dr. Andrew Gellman May, 2006 to August, 2006

- Created solid chiral stationary phases for enantiomeric selection with gas chromatography

- Tested various compounds for retention differences using solid chiral stationary phase

Carnegie Mellon University

National Chemical Car Competition

(Sponsored by AIChE) September, 2006 to May, 2008

- Captain of Carnegie Mellon University’s first chemical car team.

- Team placed first at regional competition in Spring 2006, third at regional competition in Spring 2007,

and first at the regional competition in Spring 2008.

- Team participated in national competition in Fall 2006 and placed second at the national competition in

Fall 2007.

Work Experience

Internship

ExxonMobil Corporation

May to August, 2007

Member of the Operator Guidance Technology Section, ExxonMobil Research and Engineering

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Developed mathematical models for use in abnormal event detection software

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Helped to improve toolset used to create models

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Helped to write a training manual for model development

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Publications and Presentations (note: All Publications and Presentations Listed Under Maiden Name)

Publications

1. Melissa A. Bartel, Bum-Yeol Hwang, Daniel Stone, James T. Koerber, Linda Couto, Federico Mingozzi,

Katherine A. High, and David V. Schaffer. "Directed Evolution of AAV for Enhanced Evasion of Human

Neutralizing Antibodies." (in preparation)

2. Melissa A. Bartel, Tandis Vazin, and David V. Schaffer. “Adeno-associated Viral Variant Enhances Selective

Gene Delivery to Neural Stem Cells In Vivo.”(in preparation)

3. Melissa A. Bartel, Lu Yin, Jennifer Strazzeri, William H. Merigan, and David V. Schaffer. “Effects of Systemic

Antibodies towards Intravitreal Gene Delivery Vectors in Macaques.” (in preparation)

4. Melissa A. Bartel and David V. Schaffer. “Enhanced Gene Targeting of Adult and Pluripotent Stem Cells

Using Evolved Adeno-Associated Virus.” (accepted by Methods in Molecular Biology)

5. Melissa A. Bartel*, John R. Weinstein*, and David V. Schaffer. “Directed Evolution of Novel Adeno-

associated Viruses for Therapeutic Gene Delivery”. Gene Therapy 19(6), 2012. * equal contribution

6. Prashanth Asuri,* Melissa A. Bartel*, Tandis Vazin, Jae-Hyung Jang, Tiffany B. Wong, and David V. Schaffer.

“Directed Evolution of Adeno-associated Virus for Enhanced Gene Delivery and Gene Targeting in Human

Pluripotent Stem Cells”. Molecular Therapy 20(2), 2012. * equal contribution

7. Melissa Bartel, David Schaffer, Hildegard Buning. “Enhancing the clinical potential of AAV vectors by capsid

engineering to evade pre-existing immunity.” Frontiers in Microbiology 2(204), 2011.

8. Jae-Hyung Jang, James T. Koerber, Jung-Suk Kim, Prashanth Asuri, Tandis Vazin, Melissa Bartel, Albert

Keung, Inchan Kwon, Kook In Park, and David V. Schaffer. “An Evolved Adeno-associated Viral Variant

Enhances Gene Delivery and Gene Targeting in Neural Stem Cells”. Molecular Therapy 19(4), 2011.

9. N. Shukla, M.M. Nigra, M.A. Bartel, A.M. Nigra, and A.J. Gellman. “Fe2O3 Shell Growth on Pt Nanoparticles”.

Journal of Nanoscience and Nanotechnology 11(3), 2011.

10. N. Shukla, M.M. Nigra, M.A. Bartel, T. Nuhfer, C. Phatak and A.J. Gellman. “Angle resolved TEM Imaging of

Pt Nanoparticles”. Catalysis Letters 140(3-4), 2010.

11. N. Shukla, M.A. Bartel, A.J. Gellman. “Enantioselective Separation on Chiral Au Nanoparticles”. Journal of

the American Chemical Society 132(25), 2010.

- Featured in Nanowerk.com Spotlight Series (Article Title: Fabrication of gold nanoparticles with chiral surfaces)

- F eatured in C&EN “Concentrates” p.26 88(25), 2010 (Article Title: Modified Gold Particles Serve As

C hiral A dsorbents)

12. N. Shukla, M.M. Nigra, T. Nuhfer, M.A. Bartel, A.J. Gellman. “Tailoring the shapes of FexPt100 x

nanoparticles”. Nanotechnology 20(6), 2009.

Presentations

1. Melissa A. Bartel, Bum-Yeol Hwang, Daniel Stone, James T. Koerber, Linda Couto, Federico Mingozzi,

Katherine A. High, David V. Schaffer. "Directed Evolution of AAV for Enhanced Evasion of Human

Neutralizing Antibodies." 2012 ASGCT Annual Meeting. Philadelphia, PA. May 18, 2012. Poster Presentation.

2. Melissa A. Bartel, Prashanth Asuri, Jae-Hyung Jang, David V. Schaffer. "Gene Delivery and Gene Targeting

in Human Pluriportent Stem Cells using Adeno-Associated Virus." 2012 Berkeley Stem Cell Center Retreat.

Pacific Grove, CA. April 15, 2012. Poster Presentation.

3. Melissa A. Bartel, Prashanth Asuri, Jae-Hyung Jang, Tiffany B. Wong, David V. Schaffer. "AAV-Mediated

Gene Delivery and Gene Targeting in Human Embryonic Stem Cells.” 2011 ASGCT Annual Meeting. Seattle,

WA. May 20, 2011. Poster Presentation.

4. Melissa A. Bartel, Prashanth Asuri, David V. Schaffer. "AAV-Mediated Gene Delivery and Gene Targeting in

Human Embryonic Stem Cells." 2011 Berkeley Stem Cell Center Retreat. Pacific Grove, CA. May 2, 2011.

Oral Presentation.

Leadership/ Mentor Experience

University of California, Berkeley

Graduate Student Rotation Mentor

2010, 2012

Mentored three first-year graduate students during 10-week lab rotations and a visiting scholar during a

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4-month session to give them academic lab experience

Trained students in various cell culture and molecular biology techniques

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Carnegie Mellon University

Student Section Officer

CMU American Institute of Chemical Engineers May, 2005 to May, 2008

- Held the positions of President, Vice President, and Secretary

- Organized fundraising, networking, and social events designed to encourage personal and professional

interactions between the undergraduate students, graduate students, and faculty in the Chemical

Engineering department

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