PhD

Shengzhong Su

*** **** ******* ********

Pennsylvania State University

University Park, PA 16802

814-***-****(Cell), 814-***-****(Lab)

Email: ************@*****.***

Research Experience:

My doctoral research has focused on transcriptional regulation of human microsomal epoxide hydrolase

(mEH).

• Demonstrated that transcription factors Sp1 and Sp3 are key regulators of the basal transcription

of mEH.

• Identified and characterized two distal enhancer elements in the mEH genomic region, which led

to the discovery that the master oxidative stress regulator, Nrf2 functions as a mediator of mEH

upregulation in lung cancer cells.

• Investigated the link between epigenetic modification on mEH promoter regions and tissue-

specific expression of human mEH.

Techniques and Skills:

Molecular biology: gene and recombinant DNA cloning, site-directed mutagenesis, quantitative

•

real-time PCR, chromatin immunoprecipitation assay (ChIP), electrophoretic mobility shift

assays (EMSA), luciferase reporter assays, western blotting, ELISA.

Cell biology: primary hepatocyte culture and mammalian cell culture, DNA and RNA

•

transfection, cell lysis techniques, RNAi (with siRNA, shRNA and lentivirus), multi-well plate-

based cytotoxicity and proliferation assays, basic fluorescence microscopy techniques.

Expertise in animal work: basic training on mice.

•

Computer software and analysis: R/Bioconductor for High-Throughput Sequence Analysis,

•

Identification of functional elements with ENCODE and UCSC genome browser, Microsoft

Word, Excel, and Power Point.

Research Interests:

My research interests are broadly in understanding the genetic and molecular mechanisms of human

diseases and developing new therapeutic agents by identifying potential drug targets. Specifically, I am

interested in the signal transduction pathways important for cancer and other diseases. I am also

interested in the study of how epigenetic modifications contribute to gene regulation, cancer

development and cell differentiation.

Education:

Ph.D. Molecular Toxicology, 08/2013

Huck Institute of Life Sciences, Pennsylvania State University, University Park, PA.

Thesis title: Transcriptional regulation of the human microsomal Epoxide Hydrolase gene driven by a far

upstream alternative promoter

M. S. Entomology, 12/2002

Department of Entomology, Pennsylvania State University, University Park, PA.

Thesis title: Systemic developmental resistance of gypsy moth to its baculovirus

M. S. Insect Toxicology, 06/1996

Institute of Elemento-Organic Chemistry, Nankai University, Tianjin, China

Thesis title: Studies on the Mode of Action and the Application of Insect Growth Regulators (IGRs)

B. S. Environmental Biology, 06/1993

Department of Environmental Science, Nankai University, Tianjin, China

Awards and honors:

Society of Toxicology Molecular Biology Specialty Section (MBSS) Student Award, 3rd place,

•

2011

Society of Toxicology Graduate Student Travel Award, 2010

•

Professional Affiliations:

Society of Toxicology

•

The American Society for Pharmacology and Experimental Therapeutics (ASPET)

•

Entomological Society of America

•

Entomological Society of China

•

Conferences and Presentations:

The 28th Annual Graduate Exhibition, University Park, Pennsylvania, March 24, 2013

Poster: Transcription factors Sp1 and Sp3 contribute to the basal expression of human microsomal

epoxide hydrolase driven by a far upstream promoter. Shengzhong Su and Curtis J. Omiecinski

The 52th Annual Meeting of the Society of Toxicology, San Antonio, Texas, March 10-14, 2013

Poster: Methylation profiles of CpG islands localized in the upstream regions of the human microsomal

epoxide hydrolase gene promoters. Curtis J. Omiecinski, Xiaokun Cai, Shengzhong Su and William D.

Hedrich

The 51th Annual Meeting of the Society of Toxicology, San Francisco, California, March 11-15, 2012

Poster: The Roles of Intronic DNA Elements in the Regulation of the Human Microsomal Epoxide

Hydrolase (EPHX1) Driven by an Alternative Promoter in Lung Cells. Shengzhong Su, Xi Yang, and

Curtis Omiecinski

30th Summer Symposium in Molecular Biology: Chromatin and Epigenetic Regulation of Transcription,

University Park, Pennsylvania, June 21-24, 2011.

Poster: Epigenetic modification of the alternative promoter of the human microsomal epoxide hydrolase

gene (EPHX1). Shengzhong Su, Malavika Kannuswamy, and Curtis Omiecinski.

The 50th Annual Meeting of the Society of Toxicology, Washington, D.C., March 6-10, 2011

Poster: Transcriptional regulation of the human microsomal epoxide hydrolase gene (EPHX1) driven by

a far upstream alternative promoter. Shengzhong Su, and Curtis Omiecinski

The 49th Annual Meeting of the Society of Toxicology, Salt Lake City, Utah, March 7-11, 2010

Poster: Transcriptional and tissue specific regulation of human microsomal epoxide hydrolase (EPHX1)

expression is modulated by dietary isothiocyanate derivatives. Shengzhong Su, Xi Yang, and Curtis

Omiecinski

Experimental Biology, April 28-May 2, 2007, Washington, DC

Poster: Differential splicing of the human peroxisome proliferator-activated receptor, PPARβ/δ.

Shengzhong Su and Curtis Omeicinski.

The Annual Meeting of the Entomological Society of America, December 9-12, 2001, San Diego,

California

Poster: Developmental resistance of Lymantria dispar to its baculovirus may involve an immune

response. Shengzhong Su, Mike Grove, and Kelli Hoover.

Publication:

1. SHENGZHONG SU, Xi Yang, and Curtis J. Omiecinski. The roles of intronic DNA elements in the regulation

of the human microsomal epoxide hydrolase (EPHX1) driven by a far upstream alternative promoter. (In

preparation)

2. SHENGZHONG SU, and Curtis J. Omiecinski. Transcription factors Sp1 and Sp3 contribute to the basal

expression of human microsomal epoxide hydrolase alternative E1b mRNA variant by interacting with

the proximal E1b promoter. (Submitted)

3. Scott S. Auerbach, Matthew A. Stoner, SHENGZHONG SU, and Curtis J. Omiecinski. Retinoid X Receptor-

{alpha}-Dependent Transactivation by a Naturally Occurring Structural Variant of Human Constitutive

Androstane Receptor (NR1I3). MOLECULAR PHARMACOLOGY, 2005,68:1239-1253

4. Kelli Hoover, Michael J. Grove, and SHENGZHONG SU. Systemic component to intrastadial developmental

resistance in Lymantria dispar to its baculovirus. BIOLOGICAL CONTROL, 2002, 25: 92-98

5. SHENGZHONG SU, Zhisheng Jiang, and Zhizhen Shang. Comparative studies of insecticidal effects of IGRs

on Calospilos suspecta. In THE 9TH JAPAN-CHINA SYMPOSIUM ON PESTICIDE SCIENCE, 1998,

286-92.

6. Zhisheng Jiang, Hanhong Xu, Shuqing Wan, Shanhuan Zhao, SHENGZHONG SU, et al. Effect of light-

activated compounds on superoxide dismutase activity in fourth instar Spodoptera litura Fabricius

Larvae. In THE 9TH JAPAN-CHINA SYMPOSIUM ON PESTICIDE SCIENCE, 1998, 63-70.

7. SHENGZHONG SU, Zhizhen Shang. Insecticidal effects of RH5849 on Semioihisa cinerearia Bremer et

Grey and Calospilos suspecta Warren. ENTOMOLOGICAL KNOWLEDGE, 1997, 34(4): 225-

8(Chinese)

8. Zhisheng Jiang, SHENGZHONG SU, Zhizhen Shang. Development of insect growth regulator and its

application. WORLD AGRICULTURE, 1997, (1): 46(Chinese)

9. SHENGZHONG SU, Zhishen Jiang, Zhizhen Shang. Comparative studies of insecticidal effects of RH5849

and Chlorfluazuron on Ostrinia furnacalis (Guenee), In: RESEARCH ADVANCES OF PLANT

PROTECTION IN CHINA, Beijing: China scientific and technological Press, 1996, 645-7(Chinese)

10. Zhisheng Jiang, SHENGZHONG SU, Zhizhen Shang. Comparative study on the toxicity of two kinds of

Juvenile Hormone analogs to Geometrid larvae. In: RESEARCH ADVANCES OF PLANT

PROTECTION IN CHINA, Beijing: China scientific and technological Press, 1996, 501-4(Chinese)

11. Zhizhen Shang, Zhisheng Jiang, SHENGZHONG SU. Bring the role of IGR into full play in IPM. In:

PROCEEDINGS OF THE NATIONAL SYMPOSIUM ON IPM IN CHINA, Beijing: China agricultural

scientific Press, 1996, 154-9(Chinese)

12. Jianhua Xu, Shuhua Yang, SHENGZHONG SU, Zhizhen Shang. Studies of antifeeding mechanism and

electrophysiological detection of larval Ostrinia furnacalis Guenee. In: PROCEEDINGS OF THE

NATIONAL SYMPOSIUM ON IPM IN CHINA, Beijing: China agricultural scientific Press, 1996, 154-

9(Chinese)

13. Zhizhen Shang, Jianhua Xu, Qianji Ning, SHENGZHONG SU, et al. Preliminary study on the toxicological

effect on the nymph of Periplaneta americana L. CHINESE JOURNAL OF PEST CONTROL, 1996,

12(3): 5-8(Chinese)

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