Lab Technician--Cell Culture, ELISA, BSL-2+ proficient

DONALD R. REMPINSKI

** ******** *******, ***********, ** 14225 . 716-***-**** .

************@*****.***

Research Technician with 10+ years experience in culturing mammalian,

insect, and human cancer cell lines. 10+ years experience in bacterial

cloning, requiring PCR and recombinant cell transfection. 6 years

experience in the production of pure and recombinant virus. Willing to

relocate; assistance welcome but not necessary.

Education

B.S. in Biotechnology & B.S in Applied Biology, Ferris State University,

Big Rapids, MI.

Work Experience

Research Technician (Temp: 2/2012-5/2012)

Hauptman-Woodward Research Institute, Buffalo, NY

Worked in a nonprofit biomedical research facility on research projects.

. Maintained mammalian and insect cell lines.

. Experience in protein crystallization analysis, cloning, and western

blot analysis.

Research Technician, Dept. of Cancer Biology (2009-2011)

Roswell Park Cancer Institute, Buffalo, NY

Performed assigned phases of scientific research projects in lab; organized

and analyzed laboratory research efforts; utilized quantitative and

qualitative techniques to analyze human cancer cell lines.

. Experience in cloning and the creation of recombinant cancer cell

lines, biopotency and pharmacokinetic colorimetric cellular assays,

western blot analysis, and flow cytometry.

Research Technologist (2005-2009)

Radioprotectans Group, Cleveland Biolabs, Inc., Cleveland, OH

Created recombinant Lentiviral constructs, and recombinant cell lines with

them.

. Extensive experience with ELISA assays, and with biopotency and

pharmacokinetic cellular assays.

Laboratory Technician (2000-2005)

Virus Core Facility, Cleveland Clinic Foundation, Inc., Cleveland, OH

Extensive experience in creating and producing recombinant Lentiviral and

Retroviral constructs, cloning, and creation of recombinant cell lines.

. Created, produced, and purified recombinant adenovirus and baculovirus

under BSL 2+ conditions.

. Produced and purified HPIV-3, RSV, and VSV.

PUBLICATIONS

Nithya Krishnan, Andrew Fritz, Donald Rempinski, Kieran O' Loughlin, Hans

Minderman, Ronald Berezney, William Marzluff, and Roopa Thapar. The Prolyl

Isomerase Pin1 Targets SLBP To Dissociate The SLBP-Histone mRNA Complex

Linking Histone mRNA Decay With SLBP Ubiquitination. Mol. Cell. Biol. Nov

2012 32: 4306-4322.

M S Saifo, D Rempinski, Y M Rustum, R G Azrak, 2010. Targeting the

Oncogenic Protein Beta-Catenin to Enhance Chemotherapy Outcome against

Solid Human Tumors. Molecular Cancer 9:310.

Frederic J. Reu, Douglas W. Leaman, Ratan R. Maitra, Soo In Bae, Leonid

Cherkassky, Mark W. Fox, Donald R. Rempinski, Normand Beaulieu, A. Robert

MacLeod, and Ernest C. Borden. Expression of RASSF1A, an epigenetically

silenced tumor suppressor, overcomes resistance to apoptosis induction by

interferons. Cancer Research 66, 2785-2793, March 1, 2006.

TECHNICAL COMPETENCIES

Bacterial Cloning Producing recombinant DNA for later expression-

requires PCR, ligations, preparing DNA stocks, and

enzymatic analysis.

Biopotency assays Determination of the stability and potency of target

drugs using standard and nonstandard colorimetric

methods.

DNA stock preparation Transformation and large-scale preparation of

plasmid DNA.

ELISA assays Determination of drug antibody titer in mouse, primate,

and human serum.

Flow Cytometry Used to determine the effects of said drugs on

cell populations.

Maintenance of Cell Lines Maintenance of live and frozen stocks of each

line used.

Pharmacokinetic assays Used to determine the effects or the degradation

rate of target drugs in media over time.

Preparation of Recombinant Cloning of different genes into Lentiviral

vectors for expression in

Lentivirus mammalian cells.

Protein Crystallization Used to locate crystallized proteins under

particular growth conditions.

Prokaryotic Expression Transforming a recombinant gene into PET-49(b+)

vector for its expression and production.

Recombinant Cell Line Creating recombinant (knockout) cell lines

using cloned DNA.

Creation

Western Blot Analysis Used to determine cell protein levels after

drug treatment.

Preparation of Recombinant Adenovirus/Baculovirus

Making recombinant adeno/baculovirus by cotransfecting the gene of interest

(within a shuttle vector) and an adenoviral or baculoviral vector into

mammalian cells.

Production & Purification of Recombinant and Purified Virus

Preparation of large quantities (>10mg) of virus allowed end users to

proceed with experiments without taking the time to do so themselves, at a

lower cost and in less time than outsourcing.

Production & Purification of Hazardous Pathogens

HPIV3, VSV, RSV-Preparations accomplished under BSL-2+ conditions.

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