Biotechnology Associate Scientist

Education

M.Sc. Biochemistry (May ****, Pending Thesis), San Francisco State

University

B.Sc. Chemistry (May, 2011), California State University, Fresno

Research Skills

Research and Experimental Design

Ability to design, implement, and document complex experiments which

incorporate computational analysis utilizing spreadsheets (data

reduction) and graphical displays (data presentation) (Mac/PC, Excel,

Word, PowerPoint). Performed SOP preparation and optimization.

Experience with high throughput assay design and analysis.

Mammalian Cell-based Methods

Stock cell culture maintenance, chemical exposure testing, viability

determinations, DNA/Protein extraction, isolation, and quantitation,

transient transfection, assay endpoint determinations, theoretical and

working knowledge of hybridoma methodologies, Mini/Maxi DNA

preparations and excellent aseptic technique (In Vivo- experience with

animal injection and small animal dissection).

Analytical Methods

Immunological assays (western blot, co-immunoprecipitation, ELISA),

spectroscopic methods (FRET, FACS, NMR, UV/Vis, IR, and luminometry),

microscopy, protein and chemical kinetics studies, quantitative

titrations, HPLC, IEC and GC.

Experience

Research Associate 01/2012-04/2013

Bionidus, San Francisco

Analysis and manipulation of transient transfection

techniques to increase efficiency and viability in

vitro.

Cultured and maintained up to five stock cell lines.

Prepared buffers and novel growth media formulations.

Aided in non-viral vector development, including novel

transfection applications of PEI and Lipopolyplex.

Performed transfection efficiency testing via

luminometry and Flow Cytometry (FACS) analysis.

Executed DNA cloning and purification, validating with

UV/Vis spectroscopy.

Recorded, analyzed and presented data pertinent to the

research goals.

Graduate Teaching Assistant 01/2012-05/2013

San Francisco State University, General Chemistry I/II

Taught general principles of laboratory safety,

quantum mechanics, IR/UV/Vis spectroscopy, qualitative

and quantitative titration, Redox potential and

quantitative analysis.

Senior Undergraduate Research Assistant 10/2007-08/2011

California State University, Fresno

Studied Amyloid Precursor Protein (APP) as a major

contributor to neuronal plaques causing Alzheimer's

disease.

Maintained and transfected neuronal cell lines.

Performed lysing, cell scraping and protein

quantification.

Carried out SDS-PAGE and western blot analysis from

collected samples.

Investigated indirect interactions of APP with SOD-1,

and trafficking proteins, utilizing

co-immunoprecipitation, ELISA and FRET techniques.

Began redox studies of SOD-1 after linking an

association with APP.

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