Research Associate, stem cell,oncology, molecular biology flow cytome
Resume:
SARAH J. NEERING
** ****** ***** ******, ******, NY 14612 585-***-****
*********@*****.***
Senior Research Scientist
Dedicated scientist with exceptional organizational, troubleshooting and
problem solving skills. Extensive expertise in stem cell biology, cancer
research, tissue/cell culture and processing, molecular/cellular biology,
flow cytometry, viral production/infection, animal modeling, drug delivery
and husbandry. Computer proficient in Microsoft office applications, Flow
Jo, DIVA, Cell Quest, Vector NTI and File Maker Pro.
PROFESSIONAL EXPERIENCE
Flow Cytometry Lab Manager, James P. Wilmot Cancer Center, Rochester, NY,
June 2008-June 2013
o Manage overall operation of the Cancer Center's Flow Lab.
o Operate and maintain FACS Aria II for acquisition and sorting of human
and mouse cells as well as cell lines.
o Train personnel on basic flow cytometry and usage of the LSR II.
o Utilize FACS Diva, CellQuest Pro and Flow Jo software for operating
and analyzing experiments.
o Assist researchers on experimental design of multicolor flow
cytometry, cell cycle, intracellular staining and reactive oxygen
species analysis.
o Familiar with Amnis ImageStream technology.
Senior Research Associate, University of Rochester, James P. Wilmot Cancer
Center, Rochester, NY, September 2003-June 2013
o Developed an animal model of blast crisis chronic myelogenous leukemia
(bcCML) in order to search for drugs that target leukemic stem cells
while sparing normal stem cells.
o Contributed significantly in obtaining a Department of Defense grant
to support the animal model of bcCML.
o Responsible for training graduate students, post docs and new
associates.
o Provide additional support to other researchers in the Cancer Center.
Research Associate, University of Kentucky, Markey Cancer Center,
Lexington, KY, November 1999-August 2003
o Assisted in developing a zenograft animal model of acute myelogenous
leukemia in NOD/SCID mice.
o Process bone marrow, cord blood and apheresis product for FACS
analysis and drug studies.
o Manage the lab supplies and databases.
Research Associate V, Amgen Inc., Thousand Oaks, CA, June 1998-October 1999
o Conducted pharmokinetic/dynamic studies on animals injected with
leptin-coated microparticles following GMP/GLP guidelines.
o Performed high throughput screening of colonies for unique sequences
of capsase receptors.
Research Associate, Systemix Corporation, Palo Alto, CA, March 1997-April
1998
o Worked in HIV gene therapy.
o Processed bone marrow and cord blood for HIV studies.
o Utilized flow cytometry to examine T-cell populations from HIV
patients.
Research Associate, Somatix Therapy Corporation, Alameda, CA, October 1995-
March 1997
o Employed various viral gene therapy models (Adeno-, Lenti-, Retro- and
synthetic vectors) on human stem cells.
o Applied flow cytometry methods to track gene expression in human stem
cells.
Research Associate, Aastrom Biosciences Inc., Ann Arbor, MI, February 1993-
August 1995
o Assisted in developing/testing novel bioreactors for maintaining
primitive stem cells to use for gene therapy.
o Manage laboratory and order supplies.
o Organize databases.
EDUCATION
Masters of Sciences, Biomedical Science, Western Michigan University,
Kalamazoo, MI, December 1993
Bachelor of Science, Biological Sciences, Western Michigan University,
Kalamazoo, MI, June 1988
PUBLICATIONS
Lagadinou ED, Sach A, Callahan K, Rossi RM, Neering SJ, Minhajuddin M,
Ashton JM, Pei S, Grose V, O'Dwyer KM, Leisveld JL, Brookes PS, Becker MW,
Jordan CT. "BCL-2 inhibition targets oxidative phosphorylation and
selectively eradicates quiescent human leukemia stem cells", Cell Stem
Cell, 7 March 2013, Vol. 12, No. 3, pp. 329-341.
John Ashton, Marlene Balys, Sarah Neering, Duane Hassane, G Cowley, D Root,
P Miller, B Ebert, H McMurray, Huckey Land and Craig Jordan. "Gene sets
identified with oncogene cooperativity analysis regulate in vivo growth and
survival of leukemia stem cells, Cell Stem Cell, 7 September 2012, Vol. 11,
No. 3, pp. 396-372.
Pin-Yi Wang, Fay Young, Chun-Yu Chen, Brett M. Stevens, Sarah J. Neering,
Randall M. Rossi, Timothy Bushnell, Igor Kuzin, David Heinrich, Andrea
Bottaro
and Craig T. Jordan. "The biological properties of leukemias arising from
BCR/ABL mediated transformation vary as a function of developmental origin
and activity of the p19ARF gene", Blood, 15 November 2008, Vol.112, No. 7,
pp. 4184-4192.
Sarah J Neering, Timothy Bushnell, Selcuk Sozer, John Ashton, Randall M
Rossi, Pin-Yi Wang, Deborah R Bell, David Heinrich, Andrea Bottaro, and
Craig T Jordan. "Leukemia stem cells in a genetically defined murine model
of blast crisis CML", Blood, 1 October 2007, Vol. 110, No. 7, pp. 2578-
2585.
Steven H. Nazarian, John W. Barrett, A. Michael Frace, Melissa Olsen-
Rasmussen, Nmarina Khristova, Mae Shaban, Sarah Neering, Yu Li, Inger K.
Damon, Joseph J. Esposito, Karim Essani and Grant McFadden. "Comparative
genetic analysis of genomic DNA sequences of two human isolates of Tanapox
virus", Virus Research, Vol.129, Issue 1, October 2007, pp. 11-25.
Ivan Topisirovic, Monica L. Guzman, Melanie J. McConnell, Jonathan D.
Licht, Biljana Culjkovic, Sarah J. Neering, Craig T. Jordan, and Katherine
L. B. Borden. "Aberrant Eukaryotic Translation Initiation Factor 4E-
Dependent mRNA Transport Impedes Hematopoietic Differentiation and
Contributes to Leukemogenesis", Molecular and Cellular Biology, December
2003, p. 8992-9002, Vol. 23, No. 24.
Monica L. Guzman, Sarah J. Neering, Donna Upchurch, Barry Grimes, Dianna S.
Howard, David A. Rizzieri, Selina M. Luger, and Craig T. Jordan. "Nuclear
factor-B is constitutively activated in primitive human acute myelogenous
leukemia cells", Blood, 15 October 2001, Vol. 98, No. 8, pp. 2301-2307.
PLENARY PAPER
Neering SJ, Hardy SF, Minamoto D, Spratt SK, Jordan CT. "Transduction of
primitive human hematopoietic cells with recombinant adenovirus vectors",
Blood, 1996 August 15, Vol. 88, No. 4, pp.1147-1155.
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