Maintenance Assistant

CURRICULUM VITAE

Full name: Miss. Shyamli M. Kulkarni

Father’s Name: Madhav G. Kulkarni

Cast: Open (Brahmin)

Permanent Address: “Hari Om” Wanare lay-out, Nandura road, Khamgaon.

Dist: Buldhana-444303

Maharashtra (India).

Telephone No. : 072**-******

Mobile: +91-880*******

Email: ******************@*****.***

Education qualification: M.Sc. biotechnology

Objective

To be a part of leading challenging event in ever growing and dyna mic institution a carrier which

gives me an opportunity for doing research and also help to hands on the techniques involve in it,

where I can continuously learn and contribute to the growth of Science.

Degree Year Institute attended Percentage

SEMESTER UNIVERSITY/BOARD YEAR %Marks

M.Sc. (Biotech) Amravati University (SGBAU) Summer 2013 72%

B.Sc (Biotech) Amravati University (SGBAU) Summer 2011 70%

H.S.C. Maharashtra State Board Summer 2008 65.17%

63.73%

S.S.C. Maharashtra State Board March2006

Wet lab skills

Animal cell culture:

Media preparation, membrane filtration, cell counting and viability assay, Trypsinisation and

Cryopreservation, Preparation of Primary and Secondary cell lines, subculturing and

maintenance of cell line.

Plant tissue culture:

Media preparation, Surface sterilization, Callus culture, Suspension culture, Micropropagation,

Embryogenesis, Protoplast isolation and fusion, Anther and pollen culture, Artificial seed

preparation.

Microbiology and Microbial Genetics:

Isolation and maintenance of microorganism, pure culture techniques, Growth curve kinetics,

Biochemical characterization of microorganism, Staining techniques, Antibiotics sensitivity

assays, Transformation, Transduction.

Recombinant DNA Technology and Molecular biology:

Isolation of DNA/RNA/plasmid, PCR, Blotting techniques, Qualitative and Quantitative

estimation of DNA and RNA, Competent cell preparation and transformation, Agarose gel

electrophoresis, Gel-documentation analysis, Restriction digestion and Ligation.

Biochemical and Bioprocess techniques:

Protein extraction, purification and estimation, UV Spectrophotometry, Chromatography

techniques-(Thin layer chromatography, paper chromatography), SDS-PAGE.

Immunology:

ELISA, Immunoblotting, Immunodiffusion, Immunoelectrophoresis, Blood grouping, Ag-Ab

reactions, immunodiagnostics.

Elective paper

1. Drug discovery.

2. Phytosssecondary metabolites of plants and its active compounds.

Dissertation/Project of M.Sc.

“Effect of crude extract of Aloe vera and Tinospora cordifolia on breast cancer cell line.”

Research Supervisor: Mrs. V. P. Wadegaonkar

Assistant professor

Department of Biotechnology,

S.G.B. Amravati University, Amravati,

Maharashtra, India.

Basic computer knowledge :- (MSCIT 2012)

Operating System: Windows 98, Windows XP, Windows 7, Windows Vista.

Application Software: Microsoft Office 2007.

Internet Skill: Familiarity with various Internet tools such as Browsing, E-mails, and Sequence

Retrieval from NCBI, literature search using Entrez Search engine, Sequence Analysis: BLAST,

FASTA, CLUSTAL W, MEGA.

Departmental seminars on

New delhi metallo-beta-lactamase (ndm-1): towards a new pandemia

Study of stem cell niche in bone marrow: contribute to clinical treatment

3D Bioprinting : A next generation approach to tissue engineering

I hereby declare that all information given by me above are true and correct to the best of

my knowledge.

Yours faithfully

Date: 01-09-13 Ku. Shyamli M. Kulkarni

Place : khamgaon

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