Assistant Medical

CURRICULUM VITAE

NAME: Marianna AGASSANDIAN, Ph.D.

BUSINESS ADDRESS: 3459 Fifth Avenue, NW 628 MUH

Pittsburgh, PA 15213

Office Location:

The University of Pittsburgh

Department of Medicine,

Division of Pulmonary, Allergy,

and Critical Medicine

200 Lothrop Street, 1200A BST,

Pittsburgh, PA, 15213

PERSONAL ADDRESS: 10214 Country Manor Lane

Wexford, PA 15090

PHONE OFFICE: 412-***-****

PHONE MOBILE: 319-***-****

ELECTRONIC MAIL: ************@****.***

EDUCATION AND TRAINING:

• 1982 - St.-Petersburg State University, Russia (Master degree in Biochemistry).

• 1993, June - Ph.D. Thesis in Biochemistry: "Comparative study of membranes

Mg2+- and Ca2+- ATP-ases in the different types of muscles of animals at different

levels of evolutionary development"

• 1996 – 1998 – Training in the Unit of Neurobiology and Physiopathology of

Development (INSERM- U29, 123, Bl. Port-Royal, 75014 Paris, France).

• 1998-2001 – Postdoctoral Associate in the laboratory of Alice Fulton at the

Department of Biochemistry, the University of Iowa, Iowa City, IA 52242.

Positions:

• 2010 – present – Assistant Research Professor in the laboratory of Rama Mallampalli

at the Department of Medicine, Division of Pulmonary, Allergy and Critical Care

02-29-2013

Medicine, UPMC (University of Pittsburgh Medical Center), Pittsburgh, PA

15213

• 2002 – 2010 – Assistant Research Scientist in the laboratory of Rama Mallampalli at

the Department of Internal Medicine, the University of Iowa, Iowa City, IA 52242

• 2001- 2002 - Research Investigator in the laboratory of Jackie Bickenbach at the

Department of Anatomy and Cell biology, the University of Iowa, Iowa City, IA

52242.

• 1998-2001 – Postdoctoral Associate in the laboratory of Alice Fulton at the

Department of Biochemistry, the University of Iowa, Iowa City, IA 52242.

• 1996 – 1998 – Training in the Unit of Neurobiology and Physiopathology of

Development (INSERM- U29, 123, Bl. Port-Royal, 75014 Paris, France).

• 1993 - 1997 - Research Scientist at Sechenov' Institute of Evolutionary Physiology &

Biochemistry, Russian Academy of Sciences, St.-Petersburg, Russia.

• 1993, June - Ph.D. Thesis in Biochemistry: "Comparative study of membranes

Mg2+- and Ca2+- ATP-ases in the different types of muscles of animals at different

levels of evolutionary development"

• 1986 – Junior Research Scientist at Sechenov' Institute of Evolutionary Physiology &

Biochemistry, Russian Academy of Sciences, St.-Petersburg, Russia.

• 1982-1985 - Senior Research Assistant. Sechenov' Institute of Evolutionary

Physiology& Biochemistry, Russian Academy of Sciences, St.-Petersburg,

Russia.

• 1982 - St.-Petersburg State University (Master’s degree in Biochemistry).

RESEARCH INTERESTS:

• Protein-protein interactions

• Protein translocation

• Protein ubiquitination

• Cell cycle

• Lung injury

• Regulation of surfactant metabolism in pulmonary alveolar epithelial cells

• Membrane transporters

• Regulation of ion channels and pumps

• Excitation-contraction coupling in different types of muscles

• Muscle pathologies

• Stem cells research.

EXPERIMENTAL EXPERTISE:

• Protein and lipid biochemistry, molecular biology

• Isolation and purification of RNA and DNA; PCR, RT-PCR, Real-time PCR

• Isolation and chromatographic purification of proteins; HPLC/FPLC;

02-29-2013

• Immunochemical methods: ELISA, Western blot, immunostaining;

• Isolation and purification of membrane fractions;

• Lipid analysis, TLC;

• Cell culture growing and care;

• Light, confocal and fluorescent microscopy.

PUBLICATIONS:

1. Agassandian M., Rama K. Mallampalli. Surfactant phospholipid metabolism.

Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids,

2013 March; 1831(3):612-625.

2. Agassandian M., Chen B.B, Pulijala R., Kaercher L., Glasser J. R., and

Mallampalli R.K. Calcium-calmodulin kinase 1 cooperatively regulates nucleo-

cytoplasmic shuttling of CCTα by accessing a nuclear export signal. Mol Biol

Cell, 2012; 23(14):2755-69.

3. Ray NB, Durairaj L, Chen BB, McVerry BJ, Ryan AJ, Donahoe M., Waltenbaugh

AK, O’Donnell CP, Henderson FC, Etscheidt CA, McCoy DM, Agassandian M,

Hayes-Rowan EC, Coon TA, Butler PL, Gakhar L, Mathur SN, Sieren JC,

Tyurina YY, Kagan VE, McLennan G, Mallampalli RK. Dynamic regulation of

cardiolipin by the lipid pump Atp8b1 determines the severity of lung injury in

experimental pneumonia. Nat Med. 2010 Oct;16(10):1120-7. [Epub 2010 Sep 19.]

PMID: 20852622.

Agassandian M, Chen BB, Schuster CC, Houtman JC, Mallampalli RK. 14-3-3ζ

4.

escorts CCTα for calcium-activated nuclear import in lung epithelia. FASEB J.

2010 Apr; 24(4):1271-83. [Epub 2009 Dec 9.] PMID: 20007511.

5. Agassandian M, Miakotina OL, Andrews M, Mathur SN, Mallampalli R.

Pseudomonas aeruginosa and sPLA2 IB stimulate ABCA1-mediated phospholipid

efflux via ERK-activation of PPARalpha/RXR. Biochem J. 2007 May 1;403(3):

409-20. PMID: 17223797

6. Agassandian M, Zhou J, Tephly LA, Ryan AJ, Carter AB, Mallampalli RK.

Oxysterols inhibit phosphatidylcholine synthesis via ERK docking and

phosphorylation of CTP: phosphocholine cytidylyltransferse. J Biol Chem. 2005

Jun 3;280(22): 21577-87. [Epub 2005 Mar 23.] PMID: 15788406

7. Miakotina OL, Agassandian M, Shi L, Look DC, Mallampalli RK. Adenovirus

stimulates choline efflux by increasing expression of organic cation transporter-2.

Am J Physiol Lung Cell Mol Physiol. 2005 Jan;288(1):L93-102. [Epub 2004 Sep

17.] PMID: 15377492

02-29-2013

8. Agassandian M, Mathur SN, Zhou J, Field FJ, Mallampalli RK. Oxysterols

trigger ABCA1-mediated basolateral surfactant efflux. Am J Respir Cell Mol

Biol. 2004 Aug;31(2):227-33. [Epub 2004-Mar 23.] PMID: 15039140

9. Mikhailova (Agassandian) MV, Gontareva NB, Nesterov VP. The distribution

and properties of Mg2+-ATPase in the membranes of functionally different

muscles in the hen. Zh Evol Biokhim Fiziol. 1987 Mar-Apr;23(2):180-5.

Russian. PMID: 3035829

10. Mikhailova (Agassandian) MV, Safarian EKh, Nesterov VP. Localization of

Mg2+ATPase in the membranes of the skeletal muscles and myocardium of the

frog Rana temporarial. Zh Evol Biokhim Fiziol. 1987 Mar-Apr;23(2):180-5.

CHAPTERS:

Demina I.N., Nesterov V.P., Mikhailova (Agassandian) M.V., Kostkin V.B. Influence

of hyperbaric condition on components of intracellular cation-dependent system of

muscle regulation. "Hyperbaric Physiology" Special volume, Leningrad, 1988.

Nesterov V.P., Demina I.N., Sapharian E.Ch., Mikhailova (Agassandian) M.V.,

Shmeliov V.K. Serebrenikova T.P. Study of influence of space flight factors on the

biochemical characteristics of vertebral animal muscles. Special volume of

“International Space Program,” from the Institute of Medical-Biological Problems,

the Ministry of Health, Leningrad, 2/660, 27.06.86.

ABSTRACTS:

M. Agassandian, C. Snavely, L. Kaercher, R. Pulijala, B.B. Chen, T. Coon, R.K.

Mallampalli. FBXL12 regulates G1 arrest via degradation of CaMKI. April 2013,

Boston, FASEB

M. Agassandian, C. C. Schuster, B. Chen, and R. K. Mallampalli. Ca2+ regulates CTP:

phosphocholine cytidylyltransferase nuclear translocation via binding to 14-3-3ζ

in murine lung epithelia. 2007, Washington DC, ASCB meeting.

M. Agassandian, R. K., Mallampalli. A novel pathway of whereby sPLA2 stimulates

ABCA1-mediated phospholipids efflux via ERK and PPAR /RXR. 2006, San

Francisco, The FASEB Journal, A.94, 90.46.

M. Agassandian, J. Zhou, L. A. Tephly, A. B. Carter, A. J. Ryan, R. K. Mallampalli.

02-29-2013

Oxysterols inhibit phosphocholine synthesis via ERK docking and

phosphorylation of CTP:phosphocholine cytidylyltransferase. 2005, San Diego,

The FASEB Journal, A640, 367.5

M. Agassandian, S.N. Mathur, J. Zhou, B.A. Carter, L.A. Tephly, F. Field, R.K.

Mallampalli. Surfactant phosphatidylcholine synthesis and efflux in lung epithelia

are both regulated by LXR/RXR agonists. 2004, Washington DC, The FASEB

Journal, A324, 219.1

M. Agassandian, S.N. Mathur, J. Zhou, B.A. Carter, L.A. Tephly, F. Field, R.K.

Malampalli. LXR/RXR agonists coordinately regulate surfactant

phosphatidylcholine synthesis and efflux in lung epithelia. 43rd The American

Society for Cell Biology Annual Meeting, 2003. San Francisco, P. 72. Molecular

Biology of the Cells. P.118a.656

A.B. Fulton, A. Rodriguez, M. Agassandian, C. Kim. Molecular mechanisms of muscle

induction by the Tropomyosin 3’ Untranslated region. 39th American Society for\

Cell Biology Annual Meeting, 1999. P.105a.

Agassandian Kh.V., Agassandian M.V., Der Terrossian E., Represa

A.Immunocytochemical reveal of acidic isoforms of calponin and caldesmon in

the central nervous system of rat. - 4th Annual meeting of electron microscopy

Society of Armenia. 1995. P. 13.

Mikhailova (Agassandian) M.V., Nesterov V.P. Influence of calcium channel

Blockators on activity of membrane ATP-ase systems of functionally different

muscles. These are reports of V State conference "Physiology and Biochemistry

of Mediator Processes". Moscow, 1990.

Mikhailova (Agassandian) M.V., Gontareva N.B., Nesterov V.P. Localization and

properties of Mg2+ -dependent ATP-ase in the chicken different muscle

membranes. These are reports of VI State conference of Muscles Biochemistry.

Tbilisi, 1989.

Demina I.N., Nesterov V.P., Mikhailova (Agassandian) M.V., Kostkin V.B. Influence f

hyperbaric condition on components of intracellular cation-dependent system of

muscle regulation. These are reports of State conference "Hyperbaric Physiology"

Special volume, Leningrad, 1988.

Mikhailova (Agassandian) M.V., Sapharian E.Ch., Arutunian R.S. Influence of

denervation on activities of T-system and sarcoplasmic reticulum membrane

ATP- ases of functionally different muscles. These are reports of VIII State conference

"Biophysics and Biochemistry of Biological Motility". Piston, 1987.

Nesterov V.P., Demina I.N., Sapharian E.Ch., Mikhailova (Agassandian). M.V.,

Shmeliov V.K. Serebrenikova T.P. Study of influence of space flight factors on

02-29-2013

The biochemical characteristics of vertebral animal muscles. Special report of

Institute of Medical-Biological Problems of Ministry of Health, Leningrad, 2/660,

27.06.86.

Nesterov V.P., Shmeliov V.K., Demina I.N., Mikhailova (Agassandian) M.V. Influence

of space flight factors at the condition of excitation-contraction coupling of rat

skeletal muscles. These are reports of International conference of program

"Intercosmos", Moscow, 1986.

Nesterov V.P., Sapharian E.Ch., Demina I.N., Mikhailova (Agassandian) M.V.

Formation of intracellular cation-dependent system of transmission of starting

information in process of muscles evolution. These are reports of V State

Biochemical congress. Kiev, 1986.

Nesterov V.P., Sapharian E.Ch., Demina I.N., Mikhailova (Agassandian) M.V.

Formation of cation-regulated system of intracellular meditation of surface

membrane excitation in muscles evolutionary. These are reports of V State

Biochemical Congress. Kiev, 1986.

Mikhailova (Agassandian) M.V., Nesterov V.P., Sapharian E.Ch. ATP-ase activity of

membrane preparations of lamprey's muscles. These are reports of VI State

conference of ecological physiology and biochemistry. Vilnius. 1985.

Mikhailova (Agassandian) M.V., Sapharian E.Ch. Distribution of transport ATP-ases in

skeletal muscle membranes of vertebral animals. These are reports of IV State

inter university conference of "Cell's biology". Tbilisi, 1985.

Mikhailova (Agassandian) M.V., Sapharian E.Ch. Particulars of distribution Mg2+-

ATP-ase in frog skeletal muscles membranes and myocardium. - These are

reports of V State conference of muscle biochemistry. Tbilisi, 1985.

PROFESSIONAL REFERENCES:

Rama K. Mallampalli, M.D.

Professor

Department of Medicine, Cell Biology and Molecular Physiology

Director, acute lung injury centre of Excellence

University of Pittsburgh

Chief, Pulmonary Division of the

VA Pittsburgh Healthcare System

*************@****.***

UPMC Montefiore, NW 628

3459 Fifth Avenue

02-29-2013

Pittsburgh, PA 15213

Phone: 412-***-****, FAX: 412-***-****

Bill Chen, Ph.D.

Visiting Associate Professor,

Division of Pulmonary, Allergy and Critical Care Medicine

BST-1 Room W1200 Pittsburgh, PA 15213

Phone: 412-***-****

E-mail: *****@****.***

Yutong Zhao, M.D., Ph.D.

Associate Professor of Medicine

Department of Medicine

University of Pittsburgh

3459 Fifth Ave

NW628 MUH

Pittsburgh, PA, 15213

Office phone: 412-***-****

Lab phone: 412-***-****

E-mail: ******@****.***

02-29-2013

Contact this candidate