Curriculum Vitae

Shristi S. Prakash

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***** ** *** *****, **: 206-***-****

Seattle, WA 98188 Email: ab4u25@r.postjobfree.com

EDUCATION

Undergraduate B.S. Environmental Science, 2009, University of Washington

Research supervisor: Dr. Joyce Dinglasan-Panlilio

RESEARCH EXPERIENCE

Analytical Chemist, Sterling Reference Laboratories (SAMSHA,OSHA & CAP)-Tacoma, WA.

November 2012-Presaent

• Analysis of drug metabolites in urine using Agilent 6410/6460 and AB Sciex 4500 QQQ and Q-Trap

• Quantification of data using Masshunter, Multiquant and Workstation software

• Troubleshoot and maintenance of LCMSMS (Agilent, AB Sciex and Varian) instrumentation

• Prepare reagents, calibrators and QC samples for analysis

• Perform solid phase extraction (SPE)/liquid-liquid extraction of many drug metabolites for urine and oral

fluid analysis

• Utilize Laboratory Information System (LIS) software for data entry

• Method and data validation for forensic and toxicology assays

• Method optimization of source parameters on the LCMSMS

Research Technician, Center for Urban Waters, University of Washington, WA.

August 2010-March 2012

• Help with method optimization using Agilent 5975C GCMS and Agilent 6460 LCMSMS for semi-

volatile compounds

• Sampling and extraction of air, water and sediment samples via soxlet extraction and ASE

• Analysis of sediment and air samples for volatile organic compounds (VOCs) using ChemStation

software

• Helped with lap set up and analyzed samples for TESC 433 at the University of Washington Tacoma

using analytical instrumentations such as HPLC and GC-ECD/FID

• Trained students on interpreting LCMSMS and GCMS chromatograms

• Analysis of data and incorporation into research papers for publication

• Update of standard laboratory protocols/SOPs

• Maintenance of various analytical instrumentation, including GCMS and LCMSMS

• Managed laboratory inventory and supplies

• Conducted laboratory safety training for students/interns

Undergraduate Research Assistant, University of Washington, WA.

August 2008-July 2010

• Investigated and measured different chain lengths of perfluorinated carboxylic acids or PFCAs in

• water samples collected from various locations around the Puget Sound

• Performed liquid-liquid extraction for pefluroalkyl compounds

• Water samples were analyzed via SPE and quantified using Agilent GCMS

• Performed instrument calibration (tube/auto tune), maintenance and troubleshooting

• Revised and maintained standard operating procedures (SOPs) for the University of Washington

• Tacoma Environmental Organic Chemistry (EOC) laboratory

• Helped develop and maintain the EOC Website

• Updated and maintained the laboratory chemical inventory

• Mentored and supervised students during extraction and analysis of PFCAs

PUBL ISHED ABSRACTS AND PRESENTAT IONS

Dinglasan-Panlilio, M.J., Prakash, S.S., and Baker, J.E. Perfluorinated Compounds in the Surface Waters of

Puget Sound, Washington and Clayoquot and Barkeley Sounds, British Columbia. University of Washington.

Submitted June, 2013-Marine Pollution Bulletin. Publication.

Prakash, S.S, Baker, J.E., and Dinglasan-Panlilio, M.J. Concentration of perfluorinated compounds in surface

water samples from Puget Sound, Washington. UW Water Symposium, April 18, 2011. Seattle, Washington. Poster

Presentation.

Prakash, S.S, Baker, J.E., and Dinglasan-Panlilio, M.J. Perfluorinated acids in surface waters from Puget Sound,

Washington. Pacific Northwest-Society of Environmental Toxicology and Chemistry (PNW-SETAC) 20th Annual

Meeting, April 14--16, 2011. Vancouver, Washington. Platform Presentation.

Prakash, S.S and Dinglasan-Panlilio, M.J. Baseline Measurements of perfluorinated chemicals in surface water

samples from Puget Sound, Washington. Society of Environmental Toxicology and Chemistry (SETAC) North

America 31st Annual Meeting, November 7-11, 2010. Portland, Oregon. Poster Presentation.

Prakash, S.S and Dinglasan-Panlilio, M.J. Baseline Measurements of perfluorinated chemicals in surface water

samples from Quartermaster Harbor, Puget Sound. South Sound Science Symposium, October 27, 2010. Shelton,

WA. Poster Presentation.

Prakash, S.S and Dinglasan-Panlilio, M.J. Perfluorinated carboxylic acids in water samples from Quartermaster

Harbor, Puget Sound. 8th Annual University of Washington Tacoma Environmental Research Symposium, June 3,

2010. Tacoma, Washington. Poster Presentation.

Prakash, S.S and Dinglasan-Panlilio, M.J. Determination of perfluorinated carboxylic acids in surface water

samples from Quartermaster Harbor, Puget Sound. 7th Annual University of Washington Tacoma Environmental

Research Symposium, June 9, 2009. Tacoma, Washington. Platform Presentation.

Prakash, S.S and Dinglasan-Panlilio, M.J. Perfluorinated carboxylic acids in water samples from Quartermaster

Harbor, Puget Sound. 11th Annual University of Washington Undergraduate Research Symposium, May 15, 2009.

Seattle, Washington. Poster Presentation.

AWARDS RECE I VE D

Society of Environmental Toxicology and Chemistry ($285) – Travel Award for presentation on “Baseline

Measurements of perfluorinated chemicals in surface water samples from Puget Sound, Washington”, 2010.

Society of Environmental Toxicology and Chemistry ($300) – First Place Winner for Best Undergraduate Poster at

SETAC, 2010.

University of Washington Tacoma, ($3100) – Founders Endowment Fund for Research of Perfluorochemicals in

Surface Water Samples in the Puget Sound Region, 2009.

GOVERN MENT REPORTS

Becker, B.J., Gruber, J., L i t tle, C., Buck, S., Crawford, M., Griswold, Z., I rving, T.,

Johnson, S., Patterson, K., P rakash, S.S, and Mason, B.N. 2009. Commencement

B ay (Tacoma, WA) Nearshore Salmon H abitat Assessment . Submit ted to

P ierce County Habitat Protection and Restoration Special Project.

PROFESSIONAL AFFILIATIONS

Society of Environmental Toxicology and Chemistry (2010-current)

University of Washington Alumni Association (UWAA) (2010-current)

LAB SKILLS

Liquid chromatography-tandem mass spectrometry (LCMSMS), gas chromatography mass spectrometry (GCMS),

GC-ECD/FID, HPLC, solid phase extraction, liquid-liquid extraction, chlorophyll analysis, dissolved oxygen, pH

testing, alkalinity, acid-base titration, air sampling and extraction, DNA extraction, surface and groundwater sample

collection, DNA cloning (PCR), cell cultivation and counting.

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