Training Assistant

TEQUILA V. JONES

PO Box **** North Carolina, NC *7804 252-***-**** *******.*****@*****.***

WEB LINK TO PRACTICE TRAINING SESSION:

http://www.youtube.com/watch?v=4SZkC2cRQgc&list=HL1369427565&feature=mh_lolz

OBJECTIVE:

Highly talented Microbiologist with extensive experience in studying structures and characteristics of micro-organisms, seeking Microbiologist/Research/Trainer position.

Professional Profile:

Research Microbiologist with expertise in Molecular Genetics, Quality Control, and Training.

Research Assistant (Post-doc Assist.) : Student Trainer / Teacher

Assistant Scientist

* PCR and real-time PCR

* Primer Design

* Cloning and mutation cloning

* Restricted Enzyme Assay

* Bacteria and Virus cell Culturing (adhere and suspend cell line)

* Euroley cell line CaP04 transfection

* Transformation ( chemical and electro)

* Experienced in Handling Viral Cells (HIV-1)

* Cell counting

* Cell Plating

* Ion Chromatography

* Gram staining

* Citrate Test

* KOH Mount

* Preparation of Culture media

isolation of plasma for human cells

* protein(GST, HA, Flag binding Methods), & plasmid extraction and purification

* Interpret Data

* Aseptic Techniques

* Gel and Reagents Preparation: SDS, TAE, TBS, PBS, Agar, Agarose, Lim Broth( with/without antibiotics)

* Gene annotations (Unknown gene functions)

* Automated nucleotide sequencing

* Immunoassay:

ELISA and other

assays

* Lab Safety Training

* Isolation of pure bacterial colonies

* Catalase Test

* Coagulase Test

* Hanging Drop Technique

* Pour Plate and spread plate media

* TMC Testing

* LAL Testing

* Purity Testing

* Filtration

* Plate Reading

* Recording Data

* Gowning in ISO5-ISO9 areas

* GMP Guidelines

* SOP Environment

* Laboratory Maintenance

* Ordering and stocking supplies

* Lab Cleaning

* Autoclaving Glassware, used biohazard waste, liquids and gels.

* Micro ID

* Plate Reading

* Following GMP and SOP Guidelines

* Environmental Monitoring

* Purity Testing

* Record Management

* Collecting and Recording Data

Laboratory Management

* Laboratory Maintenance

* Ordering and stocking supplies

* Lab Cleaning

* Autoclaving Glassware, used biohazard waste, liquids and gels.

* Dishwashing

* Reload and Autoclaving of tips.

* Gel and Reagents Preparation: SDS, TAE, TBS, PBS, Agar, Agarose, Lim Broth( with/without antibiotics)

* Water Filtration system cleaning once a month/ Refill of two 10gallon jugs as needed.

Aseptic Training Specialist

* Creating Curriculum

* Plate reading

* Micro-sampling

* Building training storage programs

* Micro-ID

* Gram Staining

* Sort and Build Data bases in Excel

* Run and maintain Status Reports

* Plate Reading

* Micro-sampling

* Following cGMP and SOP Guidelines

* Training and Administrating Test

* Organizing and Overseeing Aseptic Training Program and Access

* Record Management

* Collecting and Recording Data

* Develop, update, conduct training and monitor adherence to all SOP's

* Create, deliver and administer refresher cGMP training programs in accordance with CFR 210, 211.

* Prepare training metrics.

Professional Experience:

Hospira, Rocky Mount, NC

December 2011 - Present

Trainer – Aseptic and Isolator Training Specialist

Responsibilities:

* Responsible for the identification, coordination, and oversight of training programs for Aseptic/Isolator area.

* Align with functional managers on the management of training records to ensure that individual training plans and training records are accurate and up to date.

* Design, develop and deliver programs/curriculum to meet training needs and enhance productivity for Aseptic/ Isolator product production; develop train-the-trainer tools and modules for the Aseptic/Isolator Training Program.

* Collaborate with functional leaders to identify core competencies of assigned functional areas to develop short and long-term planning strategies and initiatives; collaborate with functional managers to assess ongoing individual training needs and to develop individual training plans.

* Develops training objectives, strategies, policies, and programs, determining the best instructional methods for completing the Aseptic Training process.

* Develop appropriate measures to ensure training initiatives are effective and in alignment with business strategy, including development and management of metrics and key performance indicators.

* Ensure that new Aseptic/Isolator associates training needs are assessed and training program completed.

* Collaborate with functional representatives to assess external service providers and/or site staff training needs and to support the development of eventual training programs for Terminal Sterilization Filling Line.

* Contribute to the development and delivery of high quality effective training programs.

* Evaluate the quality and effectiveness of trainings and the impact on performance.

* Work closely with other Training Partners, around the world with our other site locations, to exchange best practice and ensure consistency of training across cGMP.

* Evaluation of central electronic training record systems and tools to facilitate documentation and compliance

Summary:

As an the Lead Aseptic Training Specialist, I manage, supervise and train aseptic techniques to approximately 350 employees’ daily, which is vital to product quality and our company’s success. Employees are trained and monitored daily, thus enabling improvements and advancements to be implemented when needed. Annual micro-sampling/gowning is conducted to ensure technique is aseptic in ISO5- ISO9 area, according to the GMP/SOP guidelines. Working in the training department, monitoring personnel, teaching aseptic technique and evaluating understanding of aseptic technique ensures quality and product safety, the first time around.

Emergent, Lansing, MI

October 2010 - October 2011

Quality Control – Assistant Scientist

Responsibilities:

* Execute experimental tasks, obtain and interpret experimental data following guidance and assignment

* Carry out experiments using techniques such as, TMC, LAL, Micro-ID, TOC, and Purification

* Participate in preparing presentations, protocols and reports and standard operating procedures (SOPs)

* Present status update on current projects.

* Maintain regular laboratory and system functions for the group

* Ensure compliance with cGMP, FDA, and SOPs guidelines and regulations.

* Use sound judgment to resolve problems and make routine decisions

those in this job.

* Perform tests on water, and the environment to detect harmful microorganisms and to obtain information about sources of pollution and contamination.

* Clean and maintain laboratory equipment.

* Stock and rotate all prepared growth media, along with daily supplies, in refrigerators and cabinets.

* Routine monitoring of cleanroom manufacturing environments

* Aseptic gown technique skills

* Plate reads of microbial organisms.

* Adherence to all FDA, GMP regulations

* Peer Training and coaching

Summary:

As an Assistant Scientist working in the QC Microbiology Department, I perform Environmental Monitoring, in order to maintain an optimal environment needed for production of product. Everyday samples are carefully collected for the production/nonproduction environment, using sterile techniques and tested after the allotted incubation time. Plates are read and results and recorded. Proper gowning is needed for ISO5- ISO9 area, according to the GMP/GLP/SOP guidelines. Finally, as an Assistant Scientist, I performed numerous tasks but not limited to, plate reading, micro ID, TMC, LAL, and purity testing.

Michigan State University, East Lansing, MI

August 2007 - June 2009

Laboratory Manager – Laboratory Technological Assistant/ Research Assistant/Trainer

Achievements:

* Promoted to Student Trainer

* Developed and designed Primer System

Responsibilities:

* Lab Management

* Maintaining Stock

* Student Training

* Primer System Development (a system developed to organize primers)

* Research Assistant

* Teacher Assistant

* Multi-tasking

Summary:

As a laboratory assistant, worked with the program director and post-docs to develop a unique protocol used for teaching future students. Under the direction of my superiors, independently administrated the instructions to the students. Finally, as a research assistant, performed numerous tasks but not limited to, cell cloning, protein purification, gene sequencing and analysis, and PCR amplification. The research focused on understanding the actions of Vif and APOBEC3 proteins in HIV-1 replication, while also understanding their ability to interact.

Sparrow Hospital, Lansing, MI

June 2007 – September 2008

Microbiology- Laboratory Technician

Achievements:

* Recognized for Excellent Service Toward Patients

Responsibilities:

* Prepare and Process Specimens

* Prepare Large samples of ELISA cultures

* Order Test

* Gram Staining

* Running and Maintaining Status Reports

* Stocking and Maintaining Inventory

* Anaerobic Plating

* Troubleshooting Technical Equipment

* Peer Training

* Handling Confidential Documents

Summary:

As a laboratory technician, processing and preparation of specimens were critical during final analysis of organisms. Performing microbial conformation test daily, by the Elisa method, was standard practice. The ability to differentiate between preparation methods was vital to quick, accurate results. Also skilled in troubleshooting and maintenance of technological equipment, enable a study flow of quality high-level performances and the efficient training of new and ongoing staff members.

Computer Skills:

* RDP

* NCBI

* TIGR

* KEGG

* OMIM

* Entrez

* Minitab

* PSI-Blast

* Tree View

* Gen Bank

* Pub Med

* Gene Pix Pro 5.0

* analysis of COGS and CDD

* Windows 7

* Windows Vista

* Windows Xp

* Light exposure to Mac OS

* MS Office

* MS Outlook

* Citrix Sever

* FTP clients

* LIMS

* SAP

* Isotrain

* IKAT

Education:

Michigan State University, East Lansing, MI

B.S. in Microbiology, May 2009

Certifications:

* ASTD – American Society for Training and Development member

* Master Trainer in Consulting Certified

* PDA- Parenteral Drug Association (certified and member)

* PDA Certified in Aseptic Processing

* Microsoft Office Projects 2007

* Bio-Net Work Certification

* Certified in Aseptic Processing

References:

References are available upon request.

Contact this candidate