Assistant Project
Resume:
RAJAT GUPTA
***** ********** ****** ****, ***** Land, TX 77498 Email: ab22yw@r.postjobfree.com Mobile: +1-607-***-****
EDUCATION University of Central Florida (UCF), Orlando, Florida August 2011 - July 2013
Masters of Science (M.S), Biotechnology Cumulative G.P.A: 3.44/4.0
Thapar University, Punjab, India July 2006 - May 2010
Bachelor of Technology (B.TECH), Biotechnology Cumulative G.P.A:7.56/10.0
WORK University of Central Florida-Health and Public Affairs, Orlando, Florida
EXPERIENCE Graduate Teaching Assistant August 2011-May 2013
Supervised a class of 50 health sciences freshmen students for immunology laboratory experiments and lectures.
University of Central Florida- College of Medicine, Orlando, Florida
Graduate Research Assistant August 2012-July 2013
Project Investigator- Dr. Sampath Parthasarathy (Associate Director for Research)
Pursued a hypothesis driven research in the field of atherosclerosis and dietary lipid peroxides on human apolipoprotein
A1 as a means to reduce the atherosclerotic effects using human colon cancer cell line as an in-vitro model system.
Performed enzyme activity assays using spectrophotometry and carried out separations of radioactive aldehydes and
carboxylic acids from crude mixtures using thin layer chromatography (TLC).
Carried out molecular biology techniques such as RNA and protein isolation, real time PCR and western blotting.
Handled apoE/PON-1 knockout mice for isolating aortas and quantifying lesions following atherogenic rich diets.
Gained hands-on-expertise in electrospray ionization mass spectrometry for separating triglycerides and phospholipids.
Graduate Research Assistant August 2011 – July 2012
Project Investigator- Dr. Yoon-Seong Kim (Assistant Professor)
Developed an independent hypothesis for a research project on Glioblastoma Multiforme by stereotactic injection
technique in adult immunocompromised mice and targeted siRNA delivery by novel therapeutic nanoparticles.
Carried out cell culture based in-vitro experiments with human astrocytoma and human embryonic kidney cell lines.
Delhi University- Department of Plant Molecular Biology, Delhi, India
Research Trainee August 2010 – April 2011
Worked on techniques such as plant tissue culture, cloning genes into plasmid, transformation of plasmid in
E.coli and genetic transformation for heat tolerance in Arabidopsis thaliana and Oryza sativa .
Performed rice callus sub-culturing, yeast-2-hybrid assays, and genetic transformation of Arabidopsis
using floral dip method and phenotype analysis for stress response.
Thapar University- Department of Biotechnology & Environmental Sciences, Punjab, India
Undergraduate Research Volunteer November 2008-March 2010
Worked o n a n i m m u n o i n f o r m a t i c s a p p r o a c h for designing epitope based vaccines against influenza A virus
using online bioinformatics tools.
• I n v e s t i g a t e d t h e presence of compounds like lactic acid from discarded foods by fermentation technology.
INDUSTRIAL BIOCON Ltd., Bangalore, India January 2009 – June 2009
INTERNSHIPS Worked under cGMP guidelines on chromatographic techniques such as affinity, ion-exchange and size exclusion
chromatography along with separation of proteins using high performance liquid chromatography (HPLC).
Performed membrane chromatography as a purification tool for therapeutic proteins and emphasizing its importance in
terms of cost efficiency when compared to traditional column chromatography.
BIOCON Ltd., Bangalore, India June 2008 – July 2008
Worked on the purification aspects of therapeutic proteins using various approaches of liquid chromatography.
Performed optimization of dynamic binding capacity for protein purification on ion-exchange resins.
AWARDS/HONORS • 2011-12: Dean’s fellowship award for pursuing research at the University of Central Florida, Orlando.
2010: Awarded 1 s t p r i z e b y I I T (Indian Institute of Technology) for project presentation at the u n i v e r s i t y ’ s
•
t e c h n i c a l e v e n t with p r o j e c t abstract published in the u n i v e r s i t y r e s e a r c h magazine.
Relevant Coursework:
SKILLS
Cell & Molecular Biology Genetic & Metabolic Engineering Agricultural Biotechnology
Plant Physiology & Plant Protection Bio-analytical Techniques and Bio-imaging Virology & Immunology
Bio-informatics web based softwares: AVANA and MUSCLE 3.1
Computer applications: Proficient in Microsoft Office and Adobe.
EXTRA Active member of International Society of Technical Engineers (ISTE) at Thapar University during 2008-09.
CURRICULAR Coordinated with Youth Welfare Committee (YWC) to set up a stall at the college cultural festival in 2007.
RAJAT GUPTA
11531 Swiftwater Bridge Lane ● Sugar Land, TX 77498
607-***-**** ● ab22yw@r.postjobfree.com
REFERENCES Dr. Sampath Parthasarathy, M.B.A.
Florida Hospital Chair in Cardiovascular Science & Associate
Director for Research
University of Central Florida- College of Medicine
6900 Lake Nona Blvd
Orlando, FL- 32827-7401
ab22yw@r.postjobfree.com
Dr. Yoon-Seong Kim, MD
Assistant Professor
University of Central Florida- College of Medicine
6900 Lake Nona Blvd
Orlando, FL- 32827-7401
ab22yw@r.postjobfree.com
Mr. Greg Weigel, M.S.
Instructor
University of Central Florida- Health and Public Affairs
4000 Central Florida Blvd
Orlando, FL- 32816-2364
ab22yw@r.postjobfree.com
Contact this candidate