Suraj Saraswat, Ph.D.

*** * ********, ********** ** Chemistry

Indiana University, Bloomington, IN-47403

email: ab1qhn@r.postjobfree.com; : 419-***-**** -

WORK EXPERIENCE:

POST DOCTORAL FELLOW, Chemical Biology

(June’ 12 ~ Present)

INDIANA UNIVERSITY, BLOOMINGTON, IN, USA

Performed in-vivo and in-vitro labeling or cross-linking of ribosomal proteins using bidendate ligand (DEST,

DSP, and BS3) and/or monodentate ligand (SSETA and SMTA) for structural elucidation of ribosome.

Characterized small molecule cross-linking for protein-protein interaction with liquid chromatography-mass

spectrometry (HPLC-ESI-Q-TOF-MS, HPLC-ESI-LTQ, HPLC-ESI-Orbitrap and HPLC-MS/MS) and studied

fundamental aspects of prokaryotic ribosomes.

Separated and characterized intact proteins (ribosomal) and peptides using (2D HPLC) multidimensional

separation (SEC, SCX, C4 and C18 RPLC) along with mass spectrometry (MS and MS/MS (De Novo

Sequencing)).

Cultured bacterial cells for the extraction and purification of Ribosomal proteins.

Synthesized and characterized amidinating reagents (DEST and SSETA) using NMR.

(Aug’ 08 ~ May’ 12)

GRADUATE RESEARCH ASSISTANT

THE UNIVERSITY OF TOLEDO, TOLEDO, OH, USA

Imaged peptides and proteins present in organs via tissue imaging using imaging mass spectrometry (Bruker

MALDI-TOF-IMS). Tissue sectioning was performed on organs obtained from sacrificed mice after perfusion.

Designed, developed, and implemented strategy for absolute quantification of peptides and proteins using HPLC-

MS. This strategy relies on the use of native fluorescence of phenylala nine, tyrosine and tryptophan and MS

intensity.

Developed methodologies using ESI-Q-TOF-MS/MS (Water’s) and MALDI-TOF-TOF MS/MS (Bruker

Daltronics ultrafleXtreme) for cancer diagnosis based on glycan detection in KB and Hela cells glycoproteins.

Developed and established strategy to study energy transfer (>75%) from fluorescent proteins (B-PE and R-PE)

to Nanoparticles (Ag and Au).

Worked as part of a global project team, collaborating closely with researchers. Investigated potential drug

candidate synthesized using HPLC-ESI-Q-TOF-MS (Water’s).

Led team of undergraduates and high school students to image sub cellular chemical entities after labeling with

dyes (FITC, RITC) using light microscopy (upright Nikon microscope).

(Aug’ 06 ~ May’ 08)

GRADUATE RESEARCH ASSISTANT

THE UNIVERSITY OF SCRANTON, PA, USA

Determined in-vivo concentration of vitamins (PK) from subjects who has been provided with diets rich in

vitamins using high performance liquid chromatography (HPLC) assay with internal standard approach.

Determined effect of chemically synthesized Quercetin and its derivatives on the oxidation of low -density

lipoproteins (LDL) and very low-density lipoproteins (VLDL) that were purified from plasma using SEC.

Operated, maintained, and troubleshoot analytical instruments (HPLC-MS) and chemistry processes.

(Jan’ 04 ~ July’ 06)

CHEMIST

SHRIRAM INSTITUTE FOR INDUSTRIAL RESEARCH, DELHI, INDIA

Determined residual pesticides, POPs, PCBs, PAHs and refrigerant gases (CFCs) GC-MS/HPLC-MS/MS.

Analyzed metal samples for physical (tensile strength, yield stress, etc.) and chemical composition (Fe, Pt, Pd,

Co, Ni etc.) based on ASTM code and other industry specifications.

Determined Flash point, Density, Viscosity, etc. for petroleum and related products.

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Suraj Saraswat, Ph.D.

800 E Kirkwood, Department of Chemistry

Indiana University, Bloomington, IN-47403

email: ab1qhn@r.postjobfree.com; : 419-***-**** -

Worked in regulatory compliance environment (GLP/GMP).

EDUCATION:

Doctor of Philosophy (Chemistry), (Aug’ 08 ~ Aug’ 12)

The University of Toledo, Toledo, OH, USA

Dissertation: Investigation of energy transfer, quantification, and localization of peptides and proteins by

fluorescence spectroscopy and liquid chromatography-mass spectrometry.

Master of Arts (Biochemistry), (Aug’ 06 ~ Aug’ 08)

The University of Scranton, Scranton, PA, USA

Thesis: Determination of antioxidant effect of polyphenols and their binding studies with LDL/VLDL.

Master of Science (Physical Chemistry), (Jul’ 01 ~ May’ 04)

University of Delhi, Delhi, India

Bachelors of Science (Analytical Chemistry), (Jul’ 98 ~ May’ 01)

University of Delhi, Delhi, India

PUBLICATIONS:

1. Suraj Saraswat, Bruce Snyder, Dragan Isailovic, “Quantification of HPLC -separated peptides by

spectrofluorimetric detection of native fluorescence and mass spectrometry”, J. Chromatogr. B., 2012, 902, 70.

2. Nidhi Jaiswal, Suraj Saraswat, Manohar Ratnam, Dragan Isailovic, “Analysis of Folate Binding Protein and

Associated N-Glycans by Mass Spectrometry”, J. Proteome Res., 2012, 11 (3), 1551-1560.

3. Saraswat, Suraj, Desireddy, Anil, Zheng, Desheng, Guo, Lijun, Lu, H. Peter, Bigioni, Terry, Isailovic, Dragan,

"Energy Transfer from Fluorescent Proteins to Metal Nanoparticles" J. Phys. Chem. C, 2011, 115 (35), 17587.

4. Dragan Isailovic, Yang Xu, Tyler Copus, Suraj Saraswat, and Surya M. Nauli, "Multimodal Spectral Imaging of

Cells Using a Transmission Diffraction Grating on a Light Microscope," Appl. Spectrosc., 65, 575-583 (2011).

5. Significant number of talks and presentations at different conferences such as ASMS, HUPO, and Pittcon.

INSTRUMENTATION:

Quadrupole-Time of Flight (Q-TOF)-MS, TOF/TOF-MS (Bruker), Ion Trap-MS, LTQ Velos, LTQ-Orbitrap,

Electrospray Ionization (ESI)-MS, Matrix Assisted Laser Desorption Ionization (MALDI) -MS, Reversed Phase

High-Performance Liquid Chromatography (RPHPLC), HPLC-MS, UPLC, Gas chromatography (GC), GC-MS,

Light Microscopy, Electron Microscopy, Time-Resolved Fluorescence Spectroscopy, FTIR, UV -Vis, NMR.

TECHNIQUES:

MALDI Tissue Imaging, Solid Phase (SPE) and Liquid-Liquid Extraction, Protein Precipitation, Size-Exclusion

Chromatography (SEC), Gel-Permeation Chromatography (GPC), SDS-PAGE, Western Blotting, Cell Culture.

SOFTWARES:

Xcaliber [MS] (Thermo), FlexControl [MS], FlexAnalysis [MS], FlexImaging [MS] (Bruker), Masslynx [MS]

(Waters), eksigent [nanoLC 2D and nanoLC ultra], LCSolution and Lab View [HPLC], Agilent Chemstation

[UPLC], Mascot, Protein Prospector, Expasy, Sequest, MS-Digest, Bio-Tools, Functional Glycomics, Sym-

glycans, Scion Image [SEM], NIS-Elements BR 3.0 [Light Microscopy], Chem Draw (2D, 3D), Origin.

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