Dr. Alaka SRIVASTAVA

**** ********** *****, *****, ** 44139

E-mail: ab1qck@r.postjobfree.com

Phone No: 440-***-****

Cell No.: 585-***-****

Citizen ship: US citizen

EMPLOYMENTS

1. Technical Support Specialist, MP Biomedicals, Solon, OH, December 2010-

Present: Working directly with the customers as well as QC department as a

technical support for Biochemical, Molecular, Immunological products.

Writing/updating MSDS using WERC-software, creating product

specification sheets, editing/creating printed copy of catalogue, edit weekly,

monthly advertisement flyers. DOT, IATA (HazMat), ISO 9001 internal auditor

certified.

2. Senior Scientist, Socratech LLC, Rochester, NY, August 2007-October 2010:

Produce recombinant proteins for the treatment of Alzheimer disease. Constructed

more than 25 plasmid to express proteins in soluble form in the media. Protein was

purified using Ion-exchange, Affinity Chromatography, Gel-filtration, Dialysis, Cut off

filters etc. Tested the purified protein molecules for ligand binding. Biotin conjugated the

Antibody and developed ELISA. Routinely prepared progress reports, and presented to

the Board Members. I was involved in industry based grant writing and helped to prepare

the SOPs for technology transfer to other facilities or for in house use.

3. Research Assistant Professor, Centre of Oral Biology, University of Rochester

Medical Centre, Rochester, NY, July 2003-July 2007: Gene expression/regulation for

saliva secretion. Fabricated microarray chip, used Affymetrix gene chips. RT-PCR, Q-

PCR, Northern for data validation. Cloned several genes of interest to study in depth.

4. Research Assistant Professor, Department of Biochemistry and Biophysics,

University of Rochester Medical Centre, Rochester, September 2002-June 2003.

Expressed several mutants of Heme-Oxygenase I and II, biliverdin reductase in E. coli,

purified His, GST tagged protein, developed in vitro enzyme assay, protein-protein

interaction etc.

5. Research Associate, Molecular Biology and Cell Biology, Brown University,

Providence, July 2000-September 2002. Cloned major genes involved in

Chlorophyll/Heme Biosynthesis Pathway, expressed them with His, GST-tag, purified,

and developed enzyme assays. Used HPLC, FPLC and self made columns. Other

techniques e.g., Southern, Northern, Western, ELISA were used to accomplish the

project.

6. Master Assistant (Equivalent to Non-tenured Assistant Professor), Department of

Bioenergetics, University of Geneva, Geneva, Switzerland, October 1992-June 2000.

Light reaction/electron transport in photosynthesis. Spectroscopical techniques were used

and the results were interpreted with the help of biophysical formulae. Collaborated with

Hensatech, UK to make Plant Efficiency Analyser (PEA). PEA is now commercially

available, taught several practical and theoretical courses at University of Geneva.

Involved in several grants.

7. Post Doctoral Fellow, Department of Biology, University of California, Los Angeles,

USA, December 1989-September 1992. Zeaxanthin, a carotenoid was detected as blue

light photoreceptor in stomatal guard cells

8. Post Doctoral Fellow, Department of Botany, Hebrew University of Jerusalem,

Israel, December 1987 - November 1989. Involvement of IP3 in volume regulation.

Educational Qualification:

Ph.D. (Biology), Banaras Hindu Univ., India (1986)

Heavy Metal Pollution

SKILLS:

1. Molecular Biology: DNA, RNA isolation, Gene cloning and expression, Southern,

Northern, PCR, RT-PCR, Q-PCR, Site Directed Mutagenesis, ready made and custom

made Microarray. Fabricated microarray chip containing genes known to be related for

saliva secretion, starting from clone selection, printing, hybridization, scanning and data

analysis. Validated the microarray results using Q-PCR. During microarray fabrication,

learn some robotic pipetting, printing.

2. Biochemistry/Biophysics: Protein expression, Purification and Characterization,

Ligand-binding, Protein-protein interaction, SDS-PAGE, Western, ELISA, Immuno-

precipitation, FPLC, HPLC, Protein Phosphorylation, Ion uptake across the membrane

using tracer technique, Immuno-histochemistry, Enzyme assay development, UV/Visible

absorption and fluorescence spectrophotometric measurements in static, time resolved

and low temperature (77k) conditions. Developed Chlorophyll a Fluorometer, which

is commercially available for evaluating the vitality of plants

3. Protein expression/Purification/Characterization: Cloned and expressed proteins in E.

coli and several mammalian cells. Optimized the conditions for cell transfection and

producing stable cell line growing either in suspension or in adherent conditions.

Optimized large scale protein expression conditions for mammalian cells in suspension

and then expended the culture either in Fernbach flask (up to 1L) or in wave bag (5-7L).

Developed protein purification either using affinity column or ion-exchange media.

Fabricated antigen columns and purified antibodies using antigen columns. Characterized

the purified protein using SDS-PAGE, 2-D-SDS-PAGE, western blotting, N-terminal

sequencing, MS/MS, size exclusion column. Developed several ELISA based assays.

4. Cell Biology and Tissue Culture: Maintained several mammalian cell lines used for

transfection, cell migration and for other physiological characterization. Isolated the plant

cells into mesophyll cells, guard cells, root cells and further sub-fractionated in

mitochondria, plasma membrane, tonoplast (vacuole), endoplasmic reticulum etc.

Isolated acinar and ductal cells of salivary glands.

5. Instrumentation: Hand made or commercially available UV Visible Absorption

Spectrophotometer, UV/Visible Fluorescence Spectrophotometer, HPLC, FPLC, and

many small instruments, which are commonly used in basic biochemistry/molecular

biology labs. I can assemble different combinations of spectroscope with the help of

fiber-optic, photomultiplier etc.

6. Writing/Editing: Wrote research findings for publications, prepared documents/grants

for submission to the funding agencies, prepared quarterly and annual project progress

reports and budget, wrote protocols for approval from the regulatory authorities, e.g.,

university, FDA, submitted progress reports within specified time line and style, SOPs

for the lab/technology transfer for cGPM run, prepared MSDS documents using WERCS

software, prepared chemical QC documents. Prepared flyers, product datasheet, catalogue

etc for marketing, involve in internal auditing for ISO 9001 certification.

7. Computation: Proficiency in the use of Microsoft office products, in-depth knowledge

of bioinformatics software and statistical analysis, extracted the chemical information using

LOLI database and created MSDS using WERCS software. AS400 (ERP System), Lotus

Note (internal data management), CRM (Customer Relation Management) etc for day to

day operation.

8. Certification: Department of Transportation (DOT), International Air Transport

Association (IATA), ISO 9001 Internal auditor.

9. Supervision/Organizer: Supervised post doctorate, graduate, undergraduate students as

well as technical staffs.

10. Foreign Languages: Hindi (fluent), French (intermediate

Scientific Projects/Grants:

1. Co-PI in the following grants funded from Swiss National Science Foundation,

• Numerical Simulation of Primary Photochemistry of Photosynthesis An Approach For

Biomonitoring, October 1999 - September 2002

• The Role of Water on the Photochemical Activity and Stability of Protein Complexes

Obtained from Biomembranes, December 1998 - November 2000,

• Numerical Simulation of Primary Photochemistry of Photosynthesis, October 1996 -

September 1999,

2. Funded from Indo Swiss Center of Biotechnology, Enhancement in Abiotic Stress Tolerance

Plants of Brassica juncea through genetic manipulation. July 2001-August 2004, I was not

able to work on this project due to my relocation to USA

3. Traveling grant from Science Society of Geneva, Switzerland to participate the American

Society of Plant Physiology meeting at Baltimore, USA in 1999.

4. Traveling grant from Gordon Conference on Tetrapyrroles, Chemistry & Biology, Newport,

RI, USA 2002

5. Traveling grant to participate the International Conference on Post- Harvest Technology

(ICPHT) Kanpur, India 2006

Work-Shops, Professional Meetings and Invited Talks:

Organized Workshop:

1 Member of the International Organizing Committee to organize the International Conference

on Post- Harvest Technology (ICPHT) – 2006 November 27-30, 2006, Kanpur, India.

2 ''IIIrd Cycle in Biological Sciences, Integrated Plant System'' in Feb 2000 at Laboratory of

Bioenergetics, University of Geneva, Switzerland.

3 The Fluorescence Transient as a Tool to Characterize and Screen Photosynthetic Samples'' in

Nov 1998 at Agricultural University of Hisar, India.

Major Invited Talk:

1 Invited Talk “Chlorophyll a Fluorescence Analysis” Graduate Student Seminar Series,

January, 2005, Cornell University, Ithaca, NY, USA.

2 During 1992-2000, 6 times gave invited talk at the Science Society of Geneva, Geneva,

Switzerland

3 Invited talk ''Stomatal Movement in Changing Environments'' at ''Round Table: Integrated

Plant Systems meeting'', Feb 2000, University of Geneva, Geneva, Switzerland.

4 Invited talk “Blue Light Photoreceptors and Stomatal Movement’ August 1993, Jawaharlal

Nehru University, New Delhi, India

5 Invited talk “Stomatal Movement’ August 1993, Banaras Hindu University, Varanasi, India

6 Invited Talk “Role of Inositol 3 Phosphate in Volume Regulation of Root Cells” February

1989, Jawaharlal Nehru University, New Delhi, India

Member of Scientific Society:

1 Indian Science Congress Association 1983-1986

2 American Society of Plant Physiology 1989-1999

3 American Society of Plant Biology 1999-2002

4 International Society for Photosynthesis Research 1995- 2001

5 Society for Plant Biochemistry and Biotechnology, Life Member Since 1998

6 Indian Society of Agricultural Biochemist, Life Member Since 2006

PUBLICATIONS Highlight

(Only Full-Length Publications are listed)

1. Sagare, AP, Bell RD, SRIVASTAVA, A, Sengillo, JD, Singh I, Nishida Y, Chow N,

Zlokovic, BV (2013) A lipoprotein receptor cluster IV mutant preferentially binds amyloid b

and regulates its clearance from the mouse brain. J. Biol. Chem 24;288(21):15154-66 .

2. A. SRIVASTAVA, D Wong, JE Melvin (2008), Age and Sex Related Changes in Human

Parotid Gland Gene Expression, Archives of Oral Biology, 53(11):1058-70.

3. A SRIVASTAVA, VG. Romanenko, M Gonzalez, and JE. Melvin, A (2008) Variant of the

Ca(2+)-Activated Cl Channel Best3 is Expressed in Mouse Exocrine Glands. Journal of

Membrane Biology, 222(1):43-54.

4. JP Reyes, P Pérez-Cornejo, CY Hernández-Carballo, Alaka SRIVASTAVA, VG

Romanenko, JE Melvin and J Arreola (2007) Atypical Permeability of P2X7 Receptors

Underlie the ATP-Activated Anion Conductance in Mouse Parotid Acinar Cells. Journal of

membrane Biology, 223(2):73-85. 2008.

5. V G Romanenko,T. Nakamoto, Alaka SRIVASTAVA, T Begenisich and JE Melvin (2007)

Regulation of Membrane Potential and Fluid Secretion by Ca2+- activated K Channels in

Mouse Submandibular Glands. J Physiol. 2007, 581:801-17.

6. T Nakamoto, Alaka SRIVASTAVA, V G. Romanenko, CE. Ovitt, P Perez-Cornejo, J

Arreola, T Begenisich, and JE. Melvin (2007) Functional and Molecular Characterization of

the Human Salivary Gland Fluid Secretion Mechanism. Am J Physiol Regul Integr Comp

Physiol. 292(6):R2380-90.

7. V G Romanenko, T Nakamoto, Alaka SRIVASTAVA, JE Melvin and T Begenisich (2006)

Molecular identification and physiological roles of parotid acinar cell maxi-K channels.

Journal of Biological Chemistry 281(38):27964-72

8. Alaka SRIVASTAVA, Vanessa Lake, Sandra M. Mayer, Robert D. Willows, and Samuel I.

Beale, (2005) The Chlamydomonas reinhardtii gtr gene encoding the tetrapyrrole

biosynthetic enzyme glutamyl-tRNA reductase: structure of the gene and characterization of

the expressed enzyme. Plant Molecular Biology, 58 (5), 643-658

9. Alaka SRIVASTAVA and Samuel I. Beale (2005), Glutamyl-t-RNA reductase of

Chlorobium vibrioforme is dissociable Homodimer that contains one tightly bound heme per

subunit. Journal of Bacteriology, 187 (13), 4444-4450

10. Luiza A Nogaj, Alaka SRIVASTAVA, Robert van Lis, Samuel I Beale (2005), Expression

of glutamyl-tRNA reductase and glutamate-1-semialdehyde aminotransferase in

Chlamydomonas reinhardtii, Plant Physiology 139 (1), 389-96

11. J.J.S. Prakash, Alaka SRIVASTAVA, R.J. Strasser and P. Mohanty (2003) Senescence-

induced alterations in the photosystem II functions of Cucumis sativus cotyledons: Probing

senescence driven alterations of photosystem II by chlorophyll a fluorescence induction O-J-

I-P transients. Indian Journal of Biochemistry and Biophysics 40, 1- 10

12. G. Schansker, Alaka SRIVASTAVA, Govindjee and R.J. Strasser (2003) Characterization of

the 820 nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea

leaves. Functional Plant Biology, 30, 785-796.

13. S. Susplugas, Alaka SRIVASTAVA, R.J. Strasser (2001) Changes in the photosynthetic

activities during several stages of vegetative growth of Spirodela polyrhiza: Effect of

chromate. J. Plant Physiol., 157, 503-512.

14. Alaka SRIVASTAVA, A. Darszon, R.J. Strasser (2001) Influence of water on the primary

photosynthetic activity of Rhodospirillum rubrum in reverse micelles. Photosynthetica, 38,

333-341

15. K.-J. Appenroth, J. Stöckel, Alaka SRIVASTAVA and R.J. Strasser (2001) Multiple effects

of chromate on the photosynthetic apparatus of Spirodela polyrhiza as probed by direct, time-

resolved chlorophyll a fluorescence measurements. Environmental Pollution 115, 49-64.

16. A. Misra, Alaka SRIVASTAVA, R.J. Strasser (2001) Utilisation of Fast Chlorophyll a

Fluorescence Technique in Assessing the Salt/Ion Sensitivity of Mung Bean and Brassica

Seedlings. J. Plant Physiology 158, 1173-1181.

17. Alaka SRIVASTAVA, Govindjee and R.J. Strasser (2000) Greening of peas: parallel

measurements on 77k emission spectra, OJIP chlorophyll a fluorescence transient, period four

oscillation of the initial fluorescence level, delayed light emission, and p700. Photosynthetica,

37, 365-392

18. Alaka SRIVASTAVA, E. Rivara-Minten, C. Obregon, A. Darszon, R.J. Strasser (1999) The

role of water on photochemical activities of membrane protein complexes of bacteria. Archs.

Sci. Genève, 52, 17-27.

19. Alaka SRIVASTAVA, A. Darszon and R.J. Strasser (1999) The Influence of Water on the

Stability and Activity of Photosynthetic Complexes, Membranes and Cells in Apolar

Systems. Archs Sci. Genève, 52, 73-99.

20. Alaka SRIVASTAVA, F. Jüttner, and Reto. J. Strasser (1998) Action of the allelochemical

fischerellin A on photosystem II. Biochim. Biophys. Acta, 1364, 326-336.

21. Alaka SRIVASTAVA, B. Guissé, H. Greppin and Reto. J. Strasser (1997) Regulation of

antenna structure and electron transport in PSII under elevated temperature probed by the fast

polyphasic Chl a fluorescence transient: OKJIP, Biochim. Biophys. Acta, 1320, 95-106.

22. Alaka SRIVASTAVA and Reto J. Strasser (1996) Stress and stress management of land

plants during a regular day. J. Plant Physiol. 148, 445-455.

23. L.D. Talbott, Alaka SRIVASTAVA and E. Zeiger (1996) Stomata from growth chamber grown

Vicia faba have enhanced sensitivity to CO2. Plant Cell and Environment, 19, 1188-1194.

24. R. J. Strasser, Alaka SRIVASTAVA and Govindjee (1995) Polyphasic chlorophyll a

fluorescence transient in plants and cyanobacteria. Photochemistry and Photobiology, 61, 32-42.

25. Alaka SRIVASTAVA, Reto J. Strasser and Govindjee (1995) Polyphasic rise of chlorophyll

a fluorescence in herbicide-resistant D1 mutants of Chlamydomonas reinhardtii.

Photosynthesis Research 43, 131-141.

26. Alaka SRIVASTAVA, H. Greppin and Reto J. Strasser (1995) The steady state chlorophyll a

fluorescence exhibits in vivo an optimum as a function of light intensity which reflects the

physiological state of the plants. Plant and Cell Physiology 36, 839-848.

27. Alaka SRIVASTAVA and E. Zeiger (1995) The inhibitor of zeaxanthin formation, dithiothretol,

inhibits blue light-stimulated stomatal opening in Vicia fab, Planta 196, 445-449.

28. Alaka SRIVASTAVA and E. Zeiger (1995) Guard cell zeaxanthin tracks photosynthetic

active radiation and stomatal apertures in Vicia faba leaves. Plant Cell and Environment 18,

813-817.

29. Alaka SRIVASTAVA, Z. Lu and E. Zeiger (1995) Modification of guard cell properties in

advance lines of Pima cotton bred for higher yields and heat resistance. Plant Science 108,

125-131.

30. Alaka SRIVASTAVA and K-J. Appenroth (1995) Interaction of EDTA and iron on the

accumulation of Cd2+ in duckweeds (Lemnaceae). J. Plant Physiology 146, 173-176.

31. Alaka SRIVASTAVA, Reto J. Strasser and Govindjee (1995) Differential effects of

dimethyl- and dichlorobenzoquinone on chlorophyll a fluorescence transient in spinach

thylakoids. J. Photochem. Photobiol. 31, 163-169.

32. Alaka SRIVASTAVA and Reto. J. Strasser (1995) How do land plants respond to stress

temperature and stress light? Arch. Sci. Genève. 48, 135-146.

33. B. Guissé, Alaka SRIVASTAVA and Reto J. Strasser (1995) The polyphasic rise of the

chlorophyll a fluorescence (O-K-J-I-P) in heat stressed leaves. Arch. Sci. Genève. 48, 147-160

34. Alaka SRIVASTAVA and E. Zeiger (1992) fast fluorescence quenching from isolated guard

cell chloroplasts of Vicia faba is induced by blue not by red light. Plant Physiology 100,

1562-1566.

35. A. Amodeo, Alaka SRIVASTAVA and E. Zeiger (1992) Vanadate inhibits blue light-

stimulated swelling of Vicia guard cell protoplasts. Plant Physiology, 100, 1567-1570.

36. Alaka SRIVASTAVA and E. Tel-Or (1992) Antioxidative enzymes response of Lemna to

environmental pollutants. The Journal of Environmental Science and Health, A27, 261-272.

37. Alaka SRIVASTAVA and E. Tel-Or (1991) Effect of some environmental pollutants on the

superoxide dismutase activity in Lema. Free Rad. Res. Comms. 12-13, 601-607.

38. Alaka SRIVASTAVA, Mark Pines and Benjamin Jacoby (1989). Enhanced K+ uptake and

phosphoinositol phosphate turn over by hypertonic mannitol shock. Physiologia Plantarum.

77, 320-325.

39. Alaka SRIVASTAVA and V.S. Jaiswal (1989) Biochemical changes in duckweed after

cadmium treatment. Enhancement in Senescence. Water Air and Soil Pollution, 50, 163-170.

40. Alaka SRIVASTAVA and V.S. Jaiswal (1989) Effect of cadmium on turion formation and

germination of Spirodela polyrrhiza L. Journal of Plant Physiology. 134, 385-387.

41. Alaka SRIVASTAVA and V.S. Jaiswal (1989), Cadmium uptake by Spirodela polyrrhiza L.

SP20. Geobios, 6, 237-240.

42. V.S. Jaiswal and Alaka SRIVASTAVA (1985), Effect of cadmium on Spirodela polyrrhiza

L. Indian Journal of Plant Nutrition. 4, 71-75.

REVIEWS IN REFERRED INTERNATIONAL JOURNALS

1 Alaka SRIVASTAVA and Reto J. Strasser (1997) Constructive and destructive action of

light on photosynthetic apparatus. Journal of Scientific and Industrial Research, 56, 133-148.

Eduardo Zeiger, Lawrence D. Talbott, Silvia Frechilla, Alaka SRIVASTAVA and Jianxin

2

Zhu (2002) The guard cell chloroplast: a perspective for the 21st century. New Phytologist,

153, 415-425

BOOK CHAPTERS

1 A SRIVASTAVA (2006) Plant Microarray: The Basics. Post-Harvest Technology and Value

Addition in Cereals, Pulses and Oilseeds. (P.S. Kendurkar, G.P. Srivastava, M. Mohan, M.

Vajpeyi, eds.) Army Printing Press, Lucknow, 27-32.

Reto J. Strasser, M. Tsimili-Michael and Alaka SRIVASTAVA (2005) Analysis of the

2

Fluorescence Transient. In Advances in Photosynthesis and Respiration Series (Govindjee,

Series ed.) Volume Title, Chlorophyll a Fluorescence: A signature of Photosynthesis (G.C.

Papageorgiou and Govindjee, Vol. eds.) Kluwer, 321-362.

Alaka SRIVASTAVA (2001) Stomatal movement in response to blue light and CO2:

3

possible involvement of zeaxanthin in signal transduction in guard cells. In, Integrated Plant

System, (H. Greppin, C. Panel, W. Broughton, R.J. Strasser, eds.) University of Geneva

Press, Chapter 2, 95-123.

Alaka SRIVASTAVA and Reto J. Strasser (2001) Biomolecules in reverse micelles. In,

4

Biophysical Processes in Living Systems. (P. Paradha Saradhi, ed) Enfield, NH: Science

Publishers, USA, 1-23.

Reto J. Strasser, Alaka SRIVASTAVA and M. Tsimilli-Michael (2000), The fluorescence

5

transient as a tool to characterize and screen photosynthetic samples. In, Probing

Photosynthesis: Mechanism, Regulation and Adaptation (M. Yunus, U. Pathre and P.

Mohanty, eds.), Taylor and Francis, UK, Chapter 25, pp 445-483.

Alaka SRIVASTAVA and Reto J. Strasser (1999). Survival Strategies of Plants to cope the

6

stress of daily atmospheric changes. In: Crop Improvement for Food Security (R.K. Behl,

M.S. Punia, B.P.S. Lather, eds). SSARM, Hisar (India) pp 60-71.

Reto J. Strasser, Alaka SRIVASTAVA and M. Tsimilli-Michael (1999) Screening the

7

Vitality and photosynthetic activity of plants by fluorescence transient. In: Crop Improvement

for Food Security (R.K. Behl, M.S. Punia, B.P.S. Lather, eds). SSARM, Hisar (India) pp 72-

115.

Reto J. Strasser, Alaka SRIVASTAVA and P. Haldimann, (1996) Licht. In: Stress bei

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Pflanzen. (C. Brunold, A. Rüegsegger and R. Brändle, eds.), Verlag Paul Haupt, Bern.

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