Brian J. Philips, PhD

**** ******* ******

Pittsburgh, PA 15227

(Phone):412-***-****,Home

(Phone):412-***-****, Cell

(E-mail):ab18l1@r.postjobfree.com

Current Research: Clinical/Translational: Biological properties of adipose-

derived stem cells (ASCs) for soft tissue reconstruction/Mechanisms of

adipose tissue resorption and retention

CURRENT EMPLOYMENT

University of Pittsburgh

Faculty Research Instructor

Department of Plastic Surgery

Advisors: J. Peter Rubin, M.D./

Kacey G. Marra, Ph.D.

PREVIOUS EMPLOYMENT

University of Pittsburgh

Research Associate

Department of Pathology

Advisor: Yuri E. Nikiforov, M.D., Ph.D.

BIOGRAPHICAL

Born: Pittsburgh, PA; August 9, 1970

Married: Kara Lynn Rawe; October 9, 1999

Children: 2 (Jaden, Nathaniel)

EDUCATION

University of Missouri-Columbia

Columbia, MO

Ph.D.

December 2001

Biochemistry

Duquesne University

Pittsburgh, PA

M.S.

May 1995

Natural & Environmental Sciences

Allegheny College

Meadville, PA

B.S.

June 1992

Biology

RESEARCH INTERESTS

*Adipose stem cells

*Mechanisms of drug/hormone action

*Gene regulation

*Cancer therapeutics

LABORATORY TECHNIQUES

General (Molecular)

Agarose gel electrophoresis/SDS-PAGE

HPLC analysis Quantitative Real-Time PCR (QPCR)

DEAE/Gel filtration chromatography

Nucleic acid isolation/purification

UV/Visible spectroscopy

Gene cloning

Western blot analysis

Specific (Molecular)

Microscopy (H&E/IF/IHC)

Sister chromatid exchange (SCE) analysis

siRNA technology

Receptor binding/Competition analysis

Radioligand synthesis

Enzyme activity analysis

In vivo (Animal)

Small animal handling/dosing (ip/sc)

Small animal ovariectomy/prostate surgery

Small animal tumor cell injections (sc)

Small animal adipose tissue injections (sc)

In vitro (Cell culture)

Cell culture/transfection/storage

Primary cell isolation/culture

Drug/UV treatment

Comet analysis/FACs analysis

RESEARCH EXPERIENCE

Graduate Research:

University of Missouri

Advisor: Dennis B. Lubahn, Ph.D.

*Doctoral Dissertation: "Binding characterization of the catechol

estrogens 4-hydroxyestrone and 4-hydroxyestradiol: In vivo and In vitro

studies"

Emphasis: The use of ER?KO mice to identify novel estrogen receptor

proteins and their potential physiological functions

Duquesne University

Advisor: Kyle W. Selcer, Ph.D.

*Master's Thesis: "Breast Cancer Studies: Regulation and biochemical

characterization of antiestrogen binding sites in rat hepatic and uterine

tissues/In vivo testing of estrone sulfatase inhibitors"

TEACHING EXPERIENCE

Teaching Assistant

Biochemistry

University of Missouri-Columbia

1996, 2000

General and Comparative Endocrinology,

Anatomy and Physiology

Duquesne University

1993, 1994

*Explained fundamental concepts and

demonstrated proper laboratory techniques

*Prepared and graded weekly lab reports

Post-Doctoral Fellow, Instructor

University of Pittsburgh

2006, 2007, 2008, 2010-2013

*Trained medical residents/grads/undergrads regarding proper laboratory

techniques

SELECT PUBLICATIONS

[1] Philips BJ, Grahovac TL, Valentin JE, Chung CW, Bliley J, Pfeifer ME,

Roy SB, Dreifuss S, Kelmedi-Doko A, Kling RE, Ravuri SK, Marra KG,

Donnenberg VS, Donnenberg AD, Rubin JP. Prevalence of endogenous CD34+

adipose stem cells predicts human fat graft retention in a xenograft model.

Plast Reconstr Surg, In Press.

[2] Philips BJ, Kling RE, Valentin JE, Chung CW, Kelmendi-Doko A, Grahovac

TL, Marra KG, Fernstrom JD, Rubin JP. In vitro effects of Neuropeptide Y on

primary cultured human adipose-derived stem cells and development of an

animal model for assessing NPY in adipose tissue engineering. Submitted.

[3] Lin YC, Ramadan M, Van Dyke M, Kokai LE, Philips BJ, Marra KG. Keratin

gel filler for peripheral nerve repair in a rodent sciatic nerve injury

model. Plast Reconstr Surg 129: 67-78, 2012.

[4] Wada T, Ihunnah CA, Gao J, Ou Z, Philips BJ, Rubin JP, Marra KG, Xie W.

Estrogen sulfotransferase inhibits adipocyte differentiation. Mol

Endocrinol 25: 1612-1623, 2011.

[5] Philips BJ, Coyle CH, Morrisroe SN, Chancellor MB, Yoshimura N.

Induction of apoptosis in human bladder cancer cells by green tea

catechins. Biomed Res, 30: 207-215, 2009.

[6] Tyagi V, Philips BJ, Su R, Smaldone MC, Erickson VL, Chancellor MB,

Yoshimura N, Tyagi P. Differential expression of functional cannabinoid

receptors in human bladder detrusor and urothelium. J Urol, 181: 1932-1938,

2009.

[7] Philips BJ, Dhir R, Hutzley J, Sen M, Kelavkar UP. Polyunsaturated

fatty acid metabolizing 15-Lipoxygenase-1 (15-LO-1) expression in normal

and tumorigenic human bladder tissues. Appl Immunohistochem Mol Morph 16:

159-164, 2008.

[8] Philips BJ, Chang AY, Dervan PB, Beerman TA. DNA damage effects of a

polyamide-CBI conjugate in SV40 virions. Mol Pharmacol 67: 877-882, 2005.

PROFESSIONAL HONORS

*T32 NIH Research Training Award, 2005-2007

*Travel Award, 2001, Endocrine Society Meeting

*Donald K. Anderson Teaching Award, 1997, University of Missouri-Columbia

*Alden Academic Scholar, 1988-1992, Allegheny College

REFERENCES

J. Peter Rubin, M.D.

Chief, Department of Plastic Surgery

University of Pittsburgh School of Medicine

100 Technology Drive/Suite 200

Pittsburgh, PA 15219

(Phone): 412-***-****

(E-mail): ab18l1@r.postjobfree.com

Kacey G. Marra, Ph.D.

Associate Professor

Departments of Plastic Surgery & Bioengineering

University of Pittsburgh School of Medicine

200 Lothrop Street

Pittsburgh, PA 15261

(Phone): 412-***-****

(E-mail): ab18l1@r.postjobfree.com

John D. Fernstrom, Ph.D.

Professor

Departments of Psychiatry & Pharmacology

University of Pittsburgh School of Medicine

Research Director, UPMC Weigh Management Center

3811 O'Hara Street

Pittsburgh, PA 15213

(Phone): 412-***-****

(E-mail): ab18l1@r.postjobfree.com

STATEMENT OF RESEARCH INTERESTS

Currently, my position as Faculty Research Instructor in the

Department of Plastic Surgery at the University of Pittsburgh involves

elucidating the molecular mechanisms of fat graft development and retention

with primary human adipose-derived stem cells (ASCs) as our model system.

Such studies, with both publications and pending submissions, have

particular relevance to clinical applications related to soft tissue

reconstruction. Specifically, my research entails 3 main projects:

I. Biomedical Translational Initiative (BTI) Project: Our lab is

currently funded by a Department of Defense grant (BTI) to examine the

efficiency of non-invasive autologous fat grafting to treat

disfiguring craniofacial injuries of US civilians. One of the

specific aims of this BTI study is to assess the biological properties

of ASCs within the fat graft and correlate these properties with

clinical outcomes (i.e., fat graft retention over time). Our

manuscript has been submitted and accepted for publication (Philips et

al., Plast Reconstr Surg, October 2013). In a related study, we are

characterizing changes in ASC function with BMI. This manuscript,

which I am a co-author, is based upon proliferation, differentiation

and cytokine secretion profiles and will be submitted later this year

(2013).

II. A. Library of Pharmacologically Active Compounds (LOPAC) Project: In

a similar manner that the identification of bone morphogenetic

proteins (BMPs) has advanced clinical applications in orthopedic and

dental surgeries, we believe such pharmacologically-active compounds

have yet to be discovered for adipose tissue. A manuscript (which I

am co-author) describing our novel methodology for compound screening

is currently in-progress and will be submitted later this year (2013).

B. Neuropeptide Y (NPY) Project: A current unmet need for optimizing

the long-term viability of soft tissue reconstructive implants is the

identification of pharmacologically-active agents that promote ASC

growth and differentiation at the transplant site, as well as adequate

vascularization of the transplant. Neuropeptide Y (NPY) is a promising

candidate both in vitro and in vivo as a promoter of pre-adipocyte

proliferation and differentiation. However, to our knowledge, its

effects have not been studied in primary cultured human ASCs. In our

studies we found evidence of both responsive and non-responsive

patients to NPY treatment, suggesting potential clinical application

for improvement of soft tissue retention. The corresponding

manuscript (which I am first author) describing the details of our

findings has been submitted for publication and is currently under

review.

III. Adipogenesis/Adipose Tissue Sub-Populations: Using an in vivo athymic

nude mouse model, the purpose of this project is to identify specific

genes (and associated signaling pathways) involved in adipogenesis and

to fluorescently-label specific, sorted sub-populations of the stromal

vascular fraction (isolated from adipose tissue) and track their

differentiation cell fate (e.g., mature adipocyte, endothelial cells,

etc) as a function of time. To our knowledge, no studies of this

nature have been conducted/published to date. The corresponding

manuscripts (which I am co-author) describing our findings will be

submitted later this year (2013).

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