JOPHIN JOHN

ab0iv8@r.postjobfree.com

Home Address:

**-** ******* **** **,

Sayreville, NJ 08872

Cell: 347-***-****

CAREER OBJECTIVE: Obtaining placement in a suitable setting.

EDUCATION: Bachelor of Science Biomedical Engineering

(Tissue and Molecular engineering track), 05/2007

School of Engineering, Rutgers University, New Brunswick, NJ

Master’s in Bioinformatics, Candidate 2014, Online, UMUC

WORK EXPERIENCE:

Upstream Manufacturing Specialist, Bristol Myers Squibb (Hopewell, NJ), Temp

Dec 11-Present

• Preparation of stock solutions and media prep.

• Used tubing sealer, welder and harvest trains.

• Cleaning, set-up of stainless steel bioreactors, SIP, CIP and pressure test.

• Set-up and operation of bioprocess equipment including depth filtration

• Passage and expansion of mammalian cell lines in seed lab.

• Writing SOP’s, working with NOVA, Vi-Cell, pH meter, conductivity meter, autoclave etc.

QC Associate I, Dendreon( Morris Plains, NJ):

Apr11- Nov 11

• Performs, as assigned, in-process and final product testing of Dendreon products (e.g. flow

cytometry, ELISA, endotoxin, Gram stain, complete blood count, viability)

• Receives and maintains test samples, reagents and reference standards used in the laboratory per

approved procedures.

• Adheres to GLP, GDP and general SOP’s in accordance with FDA guidelines.

• Excessive use of LIMS used in reporting data and data analysis.

• Performs routine equipment maintenance such as calibration and cleaning. Makes relevant

reagent used in equipment use.

• Communicates with corporate QC and ensures implementation of harmonized laboratory control

systems in the NJ Bioassay Laboratory.

Assistant Research Scientist, Huntingdon Life Sciences (Somerset, NJ):

July 10 – Apr11

• Performing 4-color or greater analysis on a Coulter FC500 or similar flow cytometer

• Staining cells for extracellular and intracellular markers.

• Using dead cell exclusion dyes for viability screens

• Handling and coordinating large numbers of samples

• Performing bioassays and tissue culture.

Quality Control Associate, International Technidyne Corporation (Edison, NJ):

December 09- July 10

• Quality control of blood testing equipment.

• Quality control of blood testing products. (Raw Material testing).

• Responsible for EM of manufacturing facilities.

Biopharmaceutical Assistant Scientist, Medarex( Bristol Myers Squibb), ( Annandale, NJ):

Aug 09- Nov 09

• Clinical manufacturing in GMP facility. Working with 350 L bioreactors.

• Following batch records and SOPs. Working with CHO cells.

• Interacting with different groups such as Microbiology, Calibration etc.

• Using HPLC, CEDEX, Bubble point tester, pH meter SCD welder etc.

Research and Development Associate, Dupont( Newark,DE):

May09-Aug 09

• Research work on fermentors upto the 10 L scale.

• Media prep and fermentor start up and shut down

• Interaction with Principal Investigators

• Working with YSI and spectrometer.

• Thawing, Seeding and innoculation of vessels, with E coli strains.

• Recording experiments and calculations on appropriate paperwork.

Upstream Cell Culture Associate, Wuxi Apptec (Philadelphia, PA):

Feb 08- Jan 09

• Work includes cell monitoring in sub cultures. Passaging, Analyzing with Nova BioFlex etc.

• Manning and operating Bioreactors upto 3500 L in a cGMP environment.

• Following SOPS and requesting DCRs.

• Interacting with other departments with work related projects such as helping Validation and QA.

• Have also Cross Trained with Product Development Dept mainly seeding and passaging CHO

cells in shakers and manning small scale bioreactors upto 10 L.

• Participated in scaling up projects. Charging, innoculation and Harvesting of bioreactors. SIP and

CIP.

• End User of Delta V.

RESEARCH EXPERINCE: Lab Assistant @ Rutgers University

Oct 06- May 07

• Develop a differentiation system for the generation of myelinating oligodendrocytes from ES.

• Design different experiments (hang drop and mono layer culture), to optimize stem cells.

• Using immuno-fluorescent microscopy to differentiate different neuronal markers and quantify.

• Analyze metabolic supernatants of the above experiments with NOVA and FACS.

• Work includes cell passaging, sorting, culturing, Elisa and media preparation

INTERNSHIP: Interned as a Research Associate @ Johnson and Johnson

(Skillman, NJ). Ran RT- PCR, FACS, Mycoplasma PCR

ELISA, MTS assay and maintained Human Stem Cell (HSC).

May-June 2007.

SKILLS: Technical: C++, C, MATLAB, Microsoft Office Suite, SAS,

Windows XP, Perl, Computer Maintenance Course

Lab: GLPs, maintaining and manipulating cell lines,

Analyzing with FACS, PCR, Nova Bio Profile Flex and

immuno fluorescence imaging and data optimization.

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