Santosh Kumar Mahto, Ph.D. **** Farleigh Rd., Columbus, OH 43221 Tel: 614-***-****; Email: ab076t@r.postjobfree.com

OBJECTIVE: Challenging research position in the pharmaceutical or biotech industries

CITIZENSHIP/VISA: Permanent resident of the United States

EDUCATION:

Doctor of Philosophy in Chemistry (Biological), Wayne State University, Detroit, MI, USA, 2009

• Dissertation title: “Exploring the roles of modified nucleotides in the bacterial decoding region and antisense applications.”

Master of Science in Chemistry (Organic), Tribhuvan University, Kathmandu, NEPAL, 1995

• Dissertation title: “Deoximation of ketoximes.”

Bachelor of Science in Chemistry, Tribhuvan University, Kathmandu, NEPAL, 1993

RESEARCH EXPERIENCE:

Post-Doctoral Fellow, Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH August 2009 –present (Mentor: Prof. Jennifer J. Ottesen)

Exploring synthetic tools for histone modification on nucleosomes and chromatin, and investigating how these modifications regulate important biological functions

• Developing total protein synthesis by solid phase ligation for site-specific incorporation of modified residues

• Advanced stepwise solid-phase peptide synthesis and thiol-mediated native chemical ligation chemistry

• Developing reversible protection strategy to improve Fmoc-SPPS thioester by the N-Acylurea approach

• Automated and manual syntheses of various peptides and peptide thioesters of histone proteins using Fmoc chemistry

Doctoral research, Department of Chemistry, Wayne State University, Detroit, MI September 2003- August 2009 (Advisor: Prof. Christine S. Chow) Investigated the role of modified nucleotides in the bacterial decoding region of 16S ribosomal RNA and antisense applications

• Biophysical and ligand binding studies with various bacterial decoding region analogues

• New synthetic methodology allowed for efficient synthesis of modified nucleotide m4Cm, m4Cm phosphoramidite and analogues

• Chemical synthesis of decoding region RNA model systems containing m4Cm and analogues

• DNAse I to compare the stability effects of cationic liposomes on bcl-2 antisense oligonucleotides

• In vitro transcription of 16S rRNA, site-specific cleavage of 16S rRNA by DNAzymes and studied by primer extension

Master’s research, Central Department of Chemistry, Tribhuvan University, Kathmandu, Nepal 1993-1995 (Advisor: Prof. Chhabi L. Gajurel) Deprotection chemistry of Ketoximes

• Advanced new synthetic methods for deprotection of ketoximes

Skills:

• Analytical and preparative RP-HPLC

• Ion exchange HPLC

• MALDI-MS

• ESI-MS

• LC-MS

• UV-melting

• Circular dichroism

• TLC

• NMR

• Flash chromatography

• UV/Vis spectroscopy

• Gel electrophoresis

• 5' and 3'-radiolabeling of DNA and RNA

• SDS- PAGE and other biochemistry techniques

SELECTED PUBLICATIONS:

• Mahto, Santosh K ; Chow, Christine. S. Probing the stabilizing effects of modified nucleotides in the bacterial decoding region of 16S ribosomal RNA. Bioorg. Med. Chem. 2013, 21, 2720-2726

• Mahto, Santosh K.; Howard, Cecil J.; Shimko, John C.; Ottesen, Jennifer J. A Reversible Protection Strategy to Improve Fmoc-SPPS of Peptide Thioesters by the N-Acylurea Approach. ChemBiochem. 2011, 12, 2488-2494.

• Mahto, Santosh K.; Chow, Christine. S. Synthesis and Solution Conformation Studies of the Modified Nucleoside N4, 2'-O-dimethylcytidine (m4Cm) and its Analogues. Bioorg. Med. Chem. 2008, 16, 8795-8800.

SELECTED PRESENTATIONS:

• Mahto, Santosh. K.; Howard, Cecil J.; Shimko, John C.; Ottesen, Jennifer J. " Protection of the Dbz Linker for Preparation of Thioesters by Fmoc SPPS " 22nd American Peptide Symposium, San Diego, CA, June 25-30, 2011, abstract # 08 (poster)

• Mahto, Santosh. K.; Chow, Christine S. "Investigation of the structural roles of nucleic acid modification in the bacterial decoding region of 16S rRNA" Ninth Annual Chemistry Graduate Student Research Symposium, Detroit, MI, October 13, 2007 (talk)

• Mahto, Santosh. K.; Chow, Christine. S. ''A study of the roles of modified nucleosides in the E. coli 16S ribosomal RNA decoding region '' Eleventh International Annual RNA meeting, June 20-25, 2006, Seattle, WA, Abstract # 266 (poster)

PROFESSIONAL ORGANIZATIONS:

• American Peptide Society member, 2011 - Present

• American Chemical Society member, 2006 - Present

• Phi Lambda Upsilon Honorary Chemical Society member, 2005 - Present

SELECTED AWARDS:

• College of Liberal Arts and Science GRA award, Wayne State University, Detroit, MI 2006-07

• Phi Lambda Upsilon Travel Award 2006

• Master Research Scholarship, National Academy of Science and Technology, Nepal,1994-95

SERVICES:

• Judge, 2012 Denman Undergraduate Research Forum, The Ohio State University, Columbus, OH

• Judge, American Chemical Society Awards in Chemical Sciences at the 2012 Ohio State Science Day, Columbus, OH

• Judge, 2010 Ohio State University Undergraduate Biochemistry Poster Session, Columbus, OH

• Judge, American Chemical Society Awards in Chemical Sciences at the 2010 Ohio State Science Day, Columbus, OH

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